Accu check aviva test strips

Weekly blood glucose measurements were taken using Accu-Check standard glucometer (range 0–40 mmol/L) with Accu-Check AVIVA test strips (Roche Diagnostics Limited, West Sussex,England).

The enzymatic Infinity Glucose Hexokinase Liquid Stable Reagent (Thermo Scientific, Middletown, VA, USA) or Accu-Check Aviva test strips (Roche, Indianapolis, IN, USA) were used to assay blood and tail blood glucose, respectively. Hemoglobin A1c (HbA1c) was measured by an enzymatic assay, and insulin by ELISA, both from CrystalChem (Elk Grove Village, IL, USA). Triglycerides (TG), and plasma cholesterol were assayed by commercial kits (Pointe Scientific, Canton, MI, USA). Liver cholesterol was assayed with a fluorometric assay (Cell Biolabs, San Diego, CA, USA). Free fatty acids (FFA) were evaluated by a fluorescent method (BioAssays Systems, Hayward, CA, USA).
Mice were deprived of food overnight to perform the glucose tolerance test (GTT) on the next day. A glucose solution was given by gavage (0.5 g/kg), as previously described [71 (link)]. Blood glucose was sampled at 0, 30, 60, 90 and 120 min by tail vein puncture and evaluated by Accu-Check Aviva test strips (Roche, Indianapolis, IN, USA).
At the end of the experiment, mice were anesthetized, and their blood collected by cardiac puncture. Red blood cells and plasma were separated by using Microvette CB 300 K2E blood separation tubes (Sarstead, Nübrecht, Germany). After perfusion with phosphate buffered saline, adipose tissue and liver were removed and stored at −80°C until further use.