Rat il 1β elisa kit

Biological samples were collected from all the subjects in all three phases of the experiment. Blood was collected through retro-orbital sinus puncture, using a capillary glass tube. Approximately 1 mL of blood was harvested in sterile vacutainers laced with 3.2% sodium citrate, to prevent coagulation. Immediately after, the vacutainers were centrifuged for 10 min, at 400 rpm, to obtain plasma samples. The processed samples were stored in sterile Eppendorf containers at −80 °C. The ELISA technique was employed to assess plasma levels of IL-1, IL-6 and TNF-α, using commercially available kits: Rat IL-1β ELISA Kit, Elabscience^® Biotechnology Inc, Wuhan, Hubei, China, Rat IL-6 ELISA Kit, Elabscience^® Biotechnology Inc, Wuhan, Hubei, China, and Rat TNF-α ELISA Kit, Elabscience^® Biotechnology Inc, Wuhan, Hubei, China.
Stimulated saliva was harvested under anaesthesia, using a citric acid solution, obtained by dissolving 8 g of citric acid in 20 mL sterile 0.9% sodium chlorite solution. After wiping the excessive citric acid solution from the oral cavity with a sterile gauze, approximately 2 mL of saliva was harvested in sterile Eppendorf containers and stored at −80 °C. The salivary levels of MMP-8 were assessed in all the three phases of the experiment using the commercially available ELISA kit: Rat MMP-8 ELISA Kit, RayBio^®, Norcross, GA, USA.

The level of Il-1β was quantified from brain homogenates and plasma samples, using Rat IL-1β ELISA Kit, E-EL-R0012 (Elabscience Biotechnology Inc.: Houston, TX, USA). The generation of a standard curve and quantification steps were executed according to the manufacturer’s handbook.

Til and Lis were given as samples by Myoford Pharma in Solan, India. MPTP hydrochloride (MPTP-HCl) was obtained from Sigma Aldrich, St. Louis, MO, USA. Both Til and Lis were solubilized in 80% ethanol. Thiobarbituric Acid Reactive Substances (TBARS) assay kit (E-BC-K298-M) was obtained from Elabscience, Houston, TX, USA, and Superoxide Dismutase (SOD) activity assay kit (CS0009) and Glutathione (GSH) assay kit (MAK364) were obtained from Sigma-Aldrich Solutions, Darmstadt, Germany. Catalase assay kit was purchased from MyBioSource Inc., San Diego, CA, USA. Rat Caspase 3 ELISA Kit (E-EL-R0160) and Rat IL-1β ELISA Kit (E-EL-R0012) were obtained from Elabscience, USA. Rat Bcl-2 ELISA Kit (E-EL-R0096) was obtained from Elabscience. TNF-α ELISA kit (MBS825075) and Nuclear Factor Kappa B ELISA Kit (MBS453975) were obtained from R&D Systems, Minneapolis, MN, USA. Anti-Akt Antibody (ab8805) was obtained from Abcam Inc., Cambridge, MA, USA. All other reagents used were of analytical grade.