Abi quantstudio machine

Manufactured by Thermo Fisher Scientific

The ABI Quantstudio machine is a real-time PCR system designed for accurate and reliable nucleic acid quantification. It provides precise detection and quantification of target DNA or RNA sequences. The Quantstudio machine employs fluorescence-based detection technology to enable sensitive and reproducible results across a wide dynamic range.

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Lab products found in correlation

Mirvana mirna isolation kit, by Thermo Fisher Scientific (1 mentions) Taqman reverse transcription reagent, by Thermo Fisher Scientific (1 mentions) Taqman real time pcr master mix, by Thermo Fisher Scientific (1 mentions) Taqman primer, by Thermo Fisher Scientific (1 mentions) Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (1 mentions) Rneasy micro kit, by Qiagen (1 mentions)

Spelling variants of this product

ABI QuantStudio 7 Real-Time PCR machine (6 citations) ABI QuantStudio 5 PCR machine (5 citations)

2 protocols using abi quantstudio machine

Quantitative microRNA Analysis Protocol

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For microRNA assays, total RNA was extracted with a mirVana miRNA Isolation Kit (Applied Biosystems) and then reverse transcribed with TaqMan Reverse Transcription reagents (Applied Biosystems). TaqMan miRNA assays were performed in an ABI Quantstudio machine (Applied Biosystems). miRNA assay results were normalized to the abundance of snoRNA202. The expression of each miRNA is presented as the fold change relative to its expression in wild-type naive T cells. For RT-PCR of non-miRNA genes, cDNA was synthesized with Superscript III/II reverse transcription (Invitrogen). The information of all primers is listed in Supplementary Table 2.

Wang H., Flach H., Onizawa M., Wei L., McManus M.T, & Weiss A. (2014). Negative Regulation of Hif1a Expression and TH17 Differentiation by Hypoxia Regulated miR-210. Nature immunology, 15(4), 393-401.

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Quantification of E3 Ubiquitin Ligase GRAIL

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Total RNA was purified using the RNeasy Micro kit (QIAGEN). cDNA was synthesized using Superscript III First-Strand Synthesis System (Invitrogen) according to the manufacturer’s instructions. To measure the E3 ubiquitin ligase, GRAIL transcript level, TaqMan Real-Time PCR was performed using TaqMan Real-Time PCR Master Mix (Invitrogen) and TaqMan primer (primer 1: 5′-GCCAATTTCCTTGTCTCCTTG-3′, primer 2: 5′-CTGCTCGAAGATTACGAAATGC-3′), and probe: 5′-/56-FAM/ACTGCCTCT/ZAN/GCTTCCTGCTTTGA/31ABkFQ/-3′ were purchased from IDT. An ABI Quantstudio machine (Applied Biosystems) was used for all quantitative PCR assays. The relative abundance of all target genes was normalized to the abundance of an internal reference gene (β2 microglobulin; β2m).

Hsu L.Y., Cheng D.A., Chen Y., Liang H.E, & Weiss A. (2017). Destabilizing the autoinhibitory conformation of Zap70 induces up-regulation of inhibitory receptors and T cell unresponsiveness. The Journal of Experimental Medicine, 214(3), 833-849.

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