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Phospho ulk1 ser555 d1h4

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Phospho-ULK1 (Ser555) (D1H4) is a primary antibody that specifically recognizes ULK1 phosphorylated at serine 555. ULK1 is a serine/threonine protein kinase that plays a key role in the initiation of autophagy.

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Lab products found in correlation

Phospho acc ser79, by Cell Signaling Technology (2 mentions) Phospho ampkα thr172 40h9, by Cell Signaling Technology (1 mentions) Alexa 647, by Jackson ImmunoResearch (1 mentions) P acc ser79, by Cell Signaling Technology (1 mentions) Cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) β actin 13e5, by Cell Signaling Technology (1 mentions) Tom20 d8t4n, by Cell Signaling Technology (1 mentions) Ampk α 23a3, by Cell Signaling Technology (1 mentions) Goat anti mouse igg hrp, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit igg hrp, by Jackson ImmunoResearch (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions) Actin ab 5, by BD (1 mentions) Total acc, by Cell Signaling Technology (1 mentions) Phospho ulk1 ser757, by Cell Signaling Technology (1 mentions) Sqstm1 p62, by BD (1 mentions)

2 protocols using phospho ulk1 ser555 d1h4

1

Comprehensive Antibody Detection Protocol

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Anti-tubulin βIII antibody (clone Tuj1; R&D Systems), at 1:20,000 for Western blot, 1:1,000 for immunoistochemistry (IHC) staining. P-AMPK thr172: phospho-AMPK-α (Thr172) (40H9) Cell Signaling, at 1:1,000. AMPK: AMPK-α (23A3) Cell Signaling, at 1:1,000. P-ACC ser 79: phospho-ACC (Ser79) Cell Signaling, at 1:1,000. ACC: anti-acetyl coenzyme A carboxylase antibody [EP687Y], AB-ab45174 Abcam, at 1:2,000. P-ULK ser555: phospho-ULK1 (Ser555) (D1H4) Cell Signaling, at 1:1,000. Caspase-3-cleaved: cleaved caspase-3 (Asp175) Cell Signaling, at 1:200. Islet antibody: Developmental Studies Hybridoma bank 39.3F7, at 1:200. Tom20: Santa Cruz Tom20 (FL-145): sc 11415, at 1:1,000 for immunofluorescence (STORM analysis) and TOM20 (D8T4N) Cell Signaling at 1:1,000 for immunoblot. β-Actin: β-Actin (13E5) Cell Signaling, at 1:20,000. Efhd1 antibody was a kind gift from the laboratory of Yasuhiro Tomooka (Tokyo University of Science), at 1:1,000. Anti-neurofilament antibody 2H3, Developmental Studies Hybridoma bank, at 1:200.
Antimouse and antirabbit antibodies conjugated with Alexa 549, Alexa 488, or Alexa 647 fluorophores were used at 1:200 (Jackson ImmunoResearch Laboratories). Secondary antibodies for Western blotting: goat antimouse IgG-HRP (JIR 155-035) and goat antirabbit IgG-HRP (JIR 111-035) from Jackson, both at 1:5,000.
Ulisse V., Dey S., Rothbard D.E., Zeevi E., Gokhman I., Dadosh T., Minis A, & Yaron A. (2020). Regulation of axonal morphogenesis by the mitochondrial protein Efhd1. Life Science Alliance, 3(7), e202000753.
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2

Immunoblot Analysis of Autophagy Proteins

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Cell lysates were prepared as described previously (11 (link)) and analyzed using 4% to 12% or 10% NuPAGE gels resolved in MES (morpholineethanesulfonic acid) running buffer (Thermo Fisher Scientific). Membranes were stained using the following antibodies: LC3B, clone 5F10 (Nanotools; 0231-100); p62/SQSTM1 (BD Bioscience; 610832); phospho-S6 Ser240/Ser244 (Cell Signaling; 2215); total S6 (54D2) mouse monoclonal antibody (MAb) (Cell Signaling; 2317); phospho-ACC Ser79 (Cell Signaling; 3661); total ACC (Cell Signaling; 3662); phospho-ULK1 Ser757 (Cell Signaling; 6888); phospho-ULK1 Ser555-D1H4 (Cell Signaling; 5869); phospho-ULK1 Ser317-D2B6Y (Cell Signaling; 12753); total ULK1-D8H5 (Cell Signaling; 8054); and actin, Ab-5 (BD Bioscience; 612656). Detection was via anti-mouse or anti-rabbit Dylight-coupled secondary antibodies and LiCor Odyssey infrared scanning.
Nwadike C., Williamson L.E., Gallagher L.E., Guan J.L, & Chan E.Y. (2018). AMPK Inhibits ULK1-Dependent Autophagosome Formation and Lysosomal Acidification via Distinct Mechanisms. Molecular and Cellular Biology, 38(10), e00023-18.
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