A solution of 20 mg/ml A. bracteosa and 100 μg/ml Ajugarin I was prepared by dissolving substances in HPLC-grade methanol, sonicated, centrifuged, and filtered through Sartolon polyamide membrane filters. Samples were eluted at the flow rate of 1 ml/min with injection volume, 50 μL by the means of Agilent Chem Station Rev. B. 02–01-SR1 (260) and Agilent 1,200 series binary gradient pump, accompanied by diode array detector (DAD) (Agilent technologies, Germany). Reversed-phase column chromatography was conducted using a Zorbax-C8 analytical column (4.6 × 250 mm, 5 µm particle size) from Agilent Technologies, Germany. The separation process involved a gradient of concentrations, specifically 10% methanol in water (designated as mobile phase A) and 100% methanol (designated as mobile phase B). A gradient volume of pure methanol (mobile phase B) in 0–20 min was 0%–100%, 100% in 20–23 min, and 0% for the last 23–27 min. Chromatogram was obtained at 220 nm. The concentration of standard was 100 ppm in methanol. The amount of Ajugarin I quantified per mg A. bracteosa was observed in µg of DW. The standard and sample were compared using retention time and comparative chromatograms.
Chem station rev b 02 01 sr1 260
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The Chem Station Rev. B. 02–01-SR1 (260) is a software application designed for data acquisition, analysis, and reporting in analytical chemistry laboratories. It provides essential functionalities for managing and processing data obtained from various analytical instruments.
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2 protocols using chem station rev b 02 01 sr1 260
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Quantification of Ajugarin I in A. bracteosa
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HPLC-Based Compound Identification
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The chromatographic analysis was carried out using HPLC Agilent 1100 integrated system equipped with a G1313A automated injector, a G1311A pump and G1315B multiwave-length diode-array detector (Agilent, Santa Clara, CA, USA). The chromatographic separation of the compounds was achieved with a reversed phase columns, ZORBAX SB-C18 (3.0 mm × 250 mm, 5 μm) from Agilent and (ODS-hypersil 2.1 mm × 100 mm 3 μm) from Thermo Fisher (Waltham, MA, USA) as well as normal phase ZORBAX-RX Sil 4.6 mm × 250 mm 5 μm and Polaris 5 Si 4.6 mm × 100 mm columns from Agilent, operating at constant room temperature (20°C). The chromatographic data were analyzed using Agilent Chemstation Rev. B.02.01-SR1 (260). The compounds under study were identified by their retention times and their UV spectral characteristics.
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