Uplansapo 40x 0.95 objective

Manufactured by Olympus

Sourced in United States

The UPlanSApo 40x/0.95 objective is a high-numerical aperture lens designed for use in microscopy. It provides a magnification of 40X and a numerical aperture of 0.95, which allows for high-resolution imaging. The objective is suitable for use in a variety of microscopy techniques, including brightfield, phase contrast, and fluorescence microscopy.

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Lab products found in correlation

Cellsens 1, by Adobe (1 mentions) Bx61 vs120 s5 virtual, by Olympus (1 mentions) Xm10 monochrome camera, by Olympus (1 mentions) Vs120 virtual slide microscope, by Olympus (1 mentions)

Close spelling variants of this product

UPlanSApo (172 citations) UPlanSApo objective (31 citations)

2 protocols using uplansapo 40x 0.95 objective

Virtual Slide Scanning Microscopy Techniques

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Microscopy was performed using an Olympus BX61 VS120-S5 Virtual Slide Scanning System with UPlanSApo 40x/0.95 objective, Olympus XM10 monochrome camera, and Olympus VS-ASW FL 2.7 imaging software. Analysis and quantification were performed using Olympus CellSens 1.13, Adobe Photoshop CC and FIJI ImageJ software.

Novak J.S., Spathis R., Dang U.J., Fiorillo A.A., Hindupur R., Tully C.B., Mázala D.A., Canessa E., Brown K.J., Partridge T.A., Hathout Y, & Nagaraju K. (2021). Interrogation of Dystrophin and Dystroglycan Complex Protein Turnover After Exon Skipping Therapy. Journal of Neuromuscular Diseases, 8(Suppl 2), S383-S402.

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Histological Analysis of Mouse Muscles

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For histological analysis, paraffin-embedded muscles from adult mice described above were sectioned and stained with haematoxylin and eosin (H&E) as far as possible using the TREAT-NMD guidelines for the DMD mouse (http://www.treat-nmd.eu/research/preclinical/dmd-sops/). The images were acquired on the VS120 virtual slide microscope (Olympus USA) using the VS-ASWFL software and UPlanSApo 40x/0.95 objective, and each muscle section was assessed for the following criteria: number of myofibers and myonuclei, myofibers with central nuclei, regenerating myofibers, and inflammatory foci. Minimal Feret’s diameter for myofiber was measured using Image J (NIH) plugin.

Leikina E., Defour A., Melikov K., Van der Meulen J.H., Nagaraju K., Bhuvanendran S., Gebert C., Pfeifer K., Chernomordik L.V, & Jaiswal J.K. (2015). Annexin A1 Deficiency does not Affect Myofiber Repair but Delays Regeneration of Injured Muscles. Scientific Reports, 5, 18246.

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