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Ape1 sirna sc 29470

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APE1 siRNA (sc-29470) is a short interfering RNA (siRNA) designed to target and silence the expression of the APE1 (Apurinic/Apyrimidinic Endonuclease 1) gene. APE1 is a multifunctional protein involved in the base excision repair pathway and the redox regulation of transcription factors.

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Lab products found in correlation

Pcdna3.1 mammalian expression plasmid, by Thermo Fisher Scientific (2 mentions) Control sirna sc 29470, by Santa Cruz Biotechnology (2 mentions) Calcium phosphate transfection kit, by Applied Biological Materials (2 mentions)

Close spelling variants of this product

SiRNAs (95 citations) APE1 siRNA (2 citations)

2 protocols using ape1 sirna sc 29470

1

Adenoviral and Lentiviral APE1 Expression

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The FLAG-tagged coding sequence of APE1 was cloned into pcDNA3.1 mammalian expression plasmid (Invitrogen, Carlsbad, CA USA). The APE1 coding sequence from pcDNA3.1/APE1 plasmid was sub-cloned using Xba I and BamH I restriction sites in the adenoviral shuttle vector pACCMV. The recombinant adenovirus expressing APE1 was generated by co-transfecting HEK-293 cells with the shuttle and backbone adenoviral pJM17 plasmids using a Calcium Phosphate Transfection kit (Applied Biological Materials Inc., Richmond, BC) (34 (link)). Lentivirus particles expressing APE1 shRNA or control shRNA were produced by VectorBuilder Inc. (Santa Clara, CA, USA) and then used to transduce OE33 and CPB cells. Control siRNA (sc-29470) and APE1 siRNA (sc-29470) were obtained from Santa Cruz Biotechnology.
Bhat A.A., Lu H., Soutto M., Capobianco A., Rai P., Zaika A, & El-Rifai W. (2018). Exposure of Barrett’s and esophageal adenocarcinoma cells to Bile acids activates EGFR-STAT3 signaling axis via induction of APE1. Oncogene, 37(46), 6011-6024.
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2

Adenoviral and Lentiviral APE1 Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The FLAG-tagged coding sequence of APE1 was cloned into pcDNA3.1 mammalian expression plasmid (Invitrogen, Carlsbad, CA USA). The APE1 coding sequence from pcDNA3.1/APE1 plasmid was sub-cloned using Xba I and BamH I restriction sites in the adenoviral shuttle vector pACCMV. The recombinant adenovirus expressing APE1 was generated by co-transfecting HEK-293 cells with the shuttle and backbone adenoviral pJM17 plasmids using a Calcium Phosphate Transfection kit (Applied Biological Materials Inc., Richmond, BC) (34 (link)). Lentivirus particles expressing APE1 shRNA or control shRNA were produced by VectorBuilder Inc. (Santa Clara, CA, USA) and then used to transduce OE33 and CPB cells. Control siRNA (sc-29470) and APE1 siRNA (sc-29470) were obtained from Santa Cruz Biotechnology.
Bhat A.A., Lu H., Soutto M., Capobianco A., Rai P., Zaika A, & El-Rifai W. (2018). Exposure of Barrett’s and esophageal adenocarcinoma cells to Bile acids activates EGFR-STAT3 signaling axis via induction of APE1. Oncogene, 37(46), 6011-6024.
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