Single-cell suspensions from normal and malignant spleen samples were fixed in ethanol and stained with APC-labeled Anti-Mouse CD45R/B220 (BD Pharmingen) and FITC labeled Anti-Mouse CD19 (BD Pharmingen) or PE labeled anti-mouse IgM (BD Pharmingen) to determine the abundant cell type present. Data acquisition was performed at the Flow Cytometry and Cellular Imaging Core Facility at MD Anderson on a FACSCalibur flow cytometer and analysis was performed with FlowJo Ssoftware.
Fitc labeled anti mouse cd19
Manufactured by BD
FITC labeled Anti-Mouse CD19 is a laboratory reagent used to identify and quantify CD19-positive cells in mouse samples. It consists of a fluorescein isothiocyanate (FITC) labeled antibody that specifically binds to the CD19 antigen expressed on the surface of mouse B cells.
Automatically generated - may contain errors
2 protocols using fitc labeled anti mouse cd19
1
Immunophenotyping of Spleen Cells
2
Immunophenotyping of Spleen Cells
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Single-cell suspensions from normal and malignant spleen samples were fixed in ethanol and stained with APC-labeled Anti-Mouse CD45R/B220 (BD Pharmingen) and FITC labeled Anti-Mouse CD19 (BD Pharmingen) or PE labeled anti-mouse IgM (BD Pharmingen) to determine the abundant cell type present. Data acquisition was performed at the Flow Cytometry and Cellular Imaging Core Facility at MD Anderson on a FACSCalibur flow cytometer and analysis was performed with FlowJo Ssoftware.
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