The following antibodies were used: rabbit anti-Vps34 (Immobilon); guinea pig anti-megalin (81 (link)); rabbit anti-clathrin (Abcam, Cambridge UK, ab 21679); rabbit anti-EEA1 and rabbit anti-Rab11 both purchased from Invitrogen, rat anti-Lamp2 (sc-19991, Santa Cruz Biotechnology), mouse-anti NKA (MerckMillipore, Germany); rabbit anti-NBC1 (Alomone labs), rabbit and guinea pig anti-SGLT2 (H. Köpsell, University Würzburg), rabbit anti-S6P (5G10), rabbit anti-phospho-S6P (D57.2.2E), both purchased from Cell Signaling Technologies; sheep anti-NDRG1, sheep anti-phospho-NDRG1 (gift of D. Alessi, University of Dundee, UK); rabbit anti-B0AT1 (82 (link)); rabbit anti-y+LAT1 (83 (link)); 4F2hc (CD98, M-20, Santa Cruz Biotechnology, Heidelberg, Germany) goat anti-Alix (N-20, sc-49267, Santa Cruz Biotechnology, Heidelberg, Germany); goat anti-Vps35 and goat anti-galectin-3 (Bio-Techne, Abingdon, UK); mouse anti-SARS-CoV-2 Nucleoprotein Antibody (ProSci, 35–579); sheep anti-N-Cadherin (R&D Systems, AF6426); rabbit anti-Megalin (Sigma, SAB1305050); mouse anti-SARS-CoV-2 spike (GeneTex, GTX632604); rabbit anti-cleaved caspase-3 (Cell Signaling, 9661). Alexa647-coupled phalloidin (ThermoFischer Scientific, Zug, Switzerland) and DAPI (Thermo Scientific, 62248) were used to stain actin and nuclei, respectively.
Sheep anti n cadherin
Manufactured by R&D Systems
Sourced in United Kingdom
Sheep anti-N-Cadherin is a primary antibody that recognizes the N-cadherin protein. N-cadherin is a cell-cell adhesion molecule that plays a role in the formation and maintenance of tissue architecture. This antibody can be used for the detection and quantification of N-cadherin in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Rabbit anti clathrin, by Abcam (1 mentions)
Mouse anti sars cov 2 spike, by GeneTex (1 mentions)
Alexa647 coupled phalloidin, by Thermo Fisher Scientific (1 mentions)
Odyssey infrared imager, by LI COR (1 mentions)
Goat anti e cadherin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using sheep anti n cadherin
1
Comprehensive Antibody Characterization for Cellular Analysis
2
Adherens Junction Internalization and Reformation
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A549 cells were seeded in 96-well plates and allowed to form monolayers. The cells were induced to internalise their adherens junctions by incubating with 2.5 mM EDTA in 100 μl serum-free medium for 1 hour. The cells were then washed twice with PBS and 100 μl fresh complete medium was added and cell-cell junctions were allowed to re-form for 0, 30, 60, and 90 minutes at 37°C. The cells were fixed at each time-point by addition of 100 μl 8% paraformaldehyde, directly into the culture medium. After quenching with 50 mM NH4Cl, the cells were blocked with 1% BSA/PBS for 1 hour at room-temperature (RT). E- and N- cadherin at the cell surface was labelled with goat anti-E cadherin (Santa Cruz) and sheep anti-N cadherin (R&D Systems) antibodies in 1% BSA/PBS, respectively. The wells were extensively washed with PBS and incubated with donkey anti-goat or anti-sheep conjugated to IRDye 800 CW plus DRAQ5 (Abcam) in 1% BSA/PBS for 1 hour at RT. The cells were extensively washed with PBS and scanned on an Odyssey Infrared Imager (Licor). The cadherin signal in each well was normalised against the DRAQ5 far-red fluorescent DNA dye signal.
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