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Boc leu arg arg amc

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Boc-Leu-Arg-Arg-AMC is a fluorogenic substrate used for the detection and quantification of trypsin-like serine proteases. It consists of the peptide sequence Boc-Leu-Arg-Arg coupled to the fluorescent reporter molecule 7-amino-4-methylcoumarin (AMC). Upon cleavage by a protease, the AMC moiety is released, resulting in an increase in fluorescence that can be measured.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Streptomycin, by Merck Group (2 mentions) Penicillin, by Merck Group (2 mentions) Cisplatin, by Merck Group (2 mentions) B ap15, by Merck Group (2 mentions) Anti gapdh, by Bioworld Technology (2 mentions) Bortezomib, by BD (2 mentions) Suc leu leu 5 al tyr aminomethylcoumarin suc llvy amc, by R&D Systems (2 mentions) Anti ha tag, by Bioworld Technology (2 mentions) Z leu leu glu amc, by R&D Systems (2 mentions)

2 protocols using boc leu arg arg amc

1

Ubiquitin-Proteasome System Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
PtPT was synthesized in our lab. Other agents are bortezomib (BD Biosciences, San Jose, CA); NEM, Penicillin, Streptomycin, Cisplatin (Sigma–Aldrich Inc., St. Louis, MO); b-AP15, Suc-Leu-Leu-V al-Tyr-aminomethylcoumarin (Suc-LLVY-AMC), Z-Leu-Leu-Glu-AMC (Z-LLE-AMC), Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC), 19S, 20S and 26S human proteasomes, HA-Ubiquitin-Vinyl Sulfone (HA-Ub-VS), K48-linked tetra-ubiquitin, ubiquitin-AMC (U550) (BostonBiochem, Cambridge, MA). Antibodies used in this study were purchased from following sources: anti-ubiquitin (P4D1), anti-p27 (F-8), anti-GFP (B-2) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-p21 Waf1/Cip1 (DCS60), anti-caspase3 (8G10), anti-caspase8 (1C12), anti-caspase9 (C9), anti-PARP, anti-K48-linkage specific polyubiquitin (D9D5), anti-phospho-histone H2AX (Ser139) (20E3), anti-phospho-ATM (Ser1981) (D6H9), anti-phospho-Chk1 (Ser345) (133D3), anti-phospho-Chk2 (Thr68) (C13C1) (Cell Signaling Technology, Beverly, MA, USA); anti-GAPDH, anti-HA-tag (Bioworld Technology, Inc., St. Louis Park, MN, USA). Propidium iodide (PI) and Annexin V-FITC/PI apoptosis detection Kit were purchased from Keygen Company (Nanjing, China). 4,6-Diamidino-2-phenylindole (DAPI) was from Invitrogen (Guangzhou, China). Enhanced chemiluminescence (ECL) reagents were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).
Zhao C., Chen X., Zang D., Lan X., Liao S., Yang C., Zhang P., Wu J., Li X., Liu N., Liao Y., Huang H., Shi X., Jiang L., Liu X., He Z., Wang X, & Liu J. (2016). Platinum-containing compound platinum pyrithione is stronger and safer than cisplatin in cancer therapy. Biochemical pharmacology, 116, 22-38.
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2

Ubiquitin-Proteasome System Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
PtPT was synthesized in our lab. Other agents are bortezomib (BD Biosciences, San Jose, CA); NEM, Penicillin, Streptomycin, Cisplatin (Sigma–Aldrich Inc., St. Louis, MO); b-AP15, Suc-Leu-Leu-V al-Tyr-aminomethylcoumarin (Suc-LLVY-AMC), Z-Leu-Leu-Glu-AMC (Z-LLE-AMC), Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC), 19S, 20S and 26S human proteasomes, HA-Ubiquitin-Vinyl Sulfone (HA-Ub-VS), K48-linked tetra-ubiquitin, ubiquitin-AMC (U550) (BostonBiochem, Cambridge, MA). Antibodies used in this study were purchased from following sources: anti-ubiquitin (P4D1), anti-p27 (F-8), anti-GFP (B-2) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-p21 Waf1/Cip1 (DCS60), anti-caspase3 (8G10), anti-caspase8 (1C12), anti-caspase9 (C9), anti-PARP, anti-K48-linkage specific polyubiquitin (D9D5), anti-phospho-histone H2AX (Ser139) (20E3), anti-phospho-ATM (Ser1981) (D6H9), anti-phospho-Chk1 (Ser345) (133D3), anti-phospho-Chk2 (Thr68) (C13C1) (Cell Signaling Technology, Beverly, MA, USA); anti-GAPDH, anti-HA-tag (Bioworld Technology, Inc., St. Louis Park, MN, USA). Propidium iodide (PI) and Annexin V-FITC/PI apoptosis detection Kit were purchased from Keygen Company (Nanjing, China). 4,6-Diamidino-2-phenylindole (DAPI) was from Invitrogen (Guangzhou, China). Enhanced chemiluminescence (ECL) reagents were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).
Zhao C., Chen X., Zang D., Lan X., Liao S., Yang C., Zhang P., Wu J., Li X., Liu N., Liao Y., Huang H., Shi X., Jiang L., Liu X., He Z., Wang X, & Liu J. (2016). Platinum-containing compound platinum pyrithione is stronger and safer than cisplatin in cancer therapy. Biochemical pharmacology, 116, 22-38.
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