Radioimmunoassay kit

Manufactured by DiaSorin

Sourced in United States, Italy

The Radioimmunoassay (RIA) kit is a laboratory equipment used for the quantitative determination of analytes in biological samples. It employs the principles of radioimmunoassay technology to measure the concentration of target molecules. The kit provides the necessary reagents and materials to perform the RIA analysis.

Automatically generated - may contain errors

Lab products found in correlation

1470 wizard, by PerkinElmer (6 mentions) 1470 wizard gamma counter, by PerkinElmer (3 mentions) Beckman synchron lx20, by Beckman Coulter (2 mentions) Automatic analyzer 7600, by Hitachi (1 mentions) Zeus 9, by Takeda Pharmaceuticals (1 mentions) Integra 800, by Roche (1 mentions) Discovery w fan beam densitometer, by Hologic (1 mentions) 7600 clinical analyzer, by Hitachi (1 mentions) Qdr 4500a fan beam densitometer, by Hologic (1 mentions) Ins irma, by Thermo Fisher Scientific (1 mentions) Quantaphase 2 folate vitamin b12 radioassay kit, by Bio-Rad (1 mentions) 25 oh d 125i ria kit, by DiaSorin (1 mentions) N tact pth assay kit, by DiaSorin (1 mentions) Enzyme immunoassay, by Cayman Chemical (1 mentions) Eia kit, by Cayman Chemical (1 mentions) Ria kit, by Cayman Chemical (1 mentions) Fetuin a, by BioVendor (1 mentions)

Spelling variants of this product

Radioimmunoassay (24 citations) 25-hydroxyvitamin D 125I radioimmunoassay kit (3 citations) I125 Radioimmunoassay IA Kit (2 citations) GammaCoat Plasma Renin Activity 125 I Radioimmunoassay Kit (2 citations)

45 protocols using radioimmunoassay kit

Periodontal Status and Vitamin D Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Periodontal examination applied the FMPE protocol, which collects probe measurements from six sites per tooth for all teeth in NHANES. Accordingly, the CDC/AAP definitions were based on measures of AL and PD at the four interproximal sites per tooth [17 (link)] Severe periodontitis was characterized as: ≥ 2 interproximal sites with attachment loss (AL) ≥ 6 mm (not on the same tooth) and ≥ 1 interproximal site with probing depth (PD) ≥ 5 mm. Mild periodontitis, moderate periodontitis, and no periodontitis definitions were described in Additional file 1: Table S1.
Using the standard protocol, serum 25(OH)D levels were tested in blood samples collected through venipuncture at mobile examination centers (MEC). Serum 25(OH)D was measured via DiaSorin radioimmunoassay kit (Diasorin, Stillwater, MN, USA) at the National Center for Environmental Health, Center for Disease Control and Prevention. As in previous studies, the serum 25(OH)D levels were classified as normal (≥ 75 nmol/ml), insufficient (50–74.9 nmol/ml), deficient (25–49.9 nmol/ml), and severely deficient (< 25 nmol/ml) [16 (link)].

Zhou F., Ma N., Su R., He X., Wang X., Zhou Y, & Shi J. (2021). Serum 25-hydroxyvitamin D is negatively associated with severe periodontitis: a cross-sectional study. BMC Oral Health, 21, 479.

+ Open protocol

+ Expand

NHANES Serum 25(OH)D Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum specimens were collected from participants at a specifically designed and equipped MEC. The NHANES Laboratory Procedures Manual (https://www.cdc.gov/nchs/data/nhanes/2017-2018/manuals/2017-MEC-Laboratory-Procedures-Manual.pdf; accessed on May 3, 2023) provides detailed instructions on specimen collection and processing. In this study, the serum 25(OH)D concentration was measured using the Diasorin radioimmunoassay kit (Stillwater, MN, USA). To ensure comparability across different survey cycles of NHANES, a regression method was used to convert the data into equivalent 25(OH)D measurements based on the established liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. This technique has been used for the analysis of circulating 25(OH)D data since 2007. The NCHS of the Centers for Disease Control and Prevention recommends using the LC-MS/MS-equivalent data for all analyses across different survey cycles of NHANES. More information is accessible online via the following link: https://www.cdc.gov/nchs/nhanes/vitamin_D/analyticalnote.aspx (accessed on May 3, 2023).

Zhang S., Gao X., Ma X., Wang J, & Zhu Y. (2024). Association Between Serum 25-Hydroxyvitamin D and Trichomonas vaginalis Infection Among American Adults: NHANES 2013-2016. Sexually transmitted diseases, 51(5).

+ Open protocol

+ Expand

Serum 25(OH)D Measurement in NHANES

Check if the same lab product or an alternative is used in the 5 most similar protocols

NHANES 2001–2006, serum 25(OH)D concentration was measured by DiaSorin radioimmunoassay kit (Stillwater, MN), but the data was converted to equivalent 25(OH)D measured value from LC-MS/MS method by regression method. From 2007 to 2010, serum 25(OH)D concentration was measured by a standardized liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. The CDC LC-MS/MS method has better analytical specificity and sensitivity compared to immunoassay methods, and fixed analytical goals for imprecision ( ≤ 10%) and bias ( ≤ 5%).

Liu Y., Yang D., Shi F., Wang F., Liu X., Wen H., Mubarik S, & Yu C. (2022). Association of Serum 25(OH)D, Cadmium, CRP With All-Cause, Cause-Specific Mortality: A Prospective Cohort Study. Frontiers in Nutrition, 9, 803985.

+ Open protocol

+ Expand

Comprehensive Metabolic Panel in Dialysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Routine laboratory tests using fasting blood samples were performed before dialysis. Serum levels of hemoglobin, glucose, blood urea nitrogen, creatinine, albumin, calcium, phosphorus, alkaline phosphatase (ALP), C-reactive protein, parathyroid hormone (PTH), total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were analyzed. Serum 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D levels were assessed using a radioimmunoassay kit (DiaSorin Inc., Stillwater, MN). Fetuin-A, osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) levels were analyzed with enzyme-linked immunosorbent assay (ELISA, BioVendor Laboratory Medicine, Modrice, Czech Republic). We additionally measured fibroblast growth factor 23 (FGF-23) levels using a Millipore FGF23 ELISA Kit (Millipore Co., Billerica, MA).

Lee S.M., Lee H.W., Son Y.K., Kim S.E, & An W.S. (2017). Abdominal aortic calcification score among several vascular calcification scores of plain radiograph is the most reliable predictor of severe coronary artery calcification in dialysis patients. Renal Failure, 39(1), 729-735.

+ Open protocol

+ Expand

Serum Biomarkers for Metabolic Health

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were obtained in the morning following an overnight fast. The serum concentrations of glucose, high-density lipoprotein cholesterol (HDL), triglycerides, aspartate transaminase (AST), and alanine transaminase (ALT) were measured using a Hitachi automatic analyzer 7600 (Tokyo, Japan). Low-density lipoprotein cholesterol (LDL) was measured directly using a Hitachi automatic analyzer 7600. Serum C-reactive proteins were measured with an ELISA kit (Abcam, Cambridge, UK). Serum 25-OH-D levels, as an indicator of vitamin D status, were measured with a radioimmunoassay kit (DiaSorin, Stillwater, MN, USA) using a 1470 Wizard gamma counter (Perkin–Elmer, Turku, Finland). All clinical analyses were performed by the Neodin Medical Institute, a laboratory certified by the Korean Ministry of Health and Welfare.

Wu X., Unno T., Kang S, & Park S. (2021). A Korean-Style Balanced Diet Has a Potential Connection with Ruminococcaceae Enterotype and Reduction of Metabolic Syndrome Incidence in Korean Adults. Nutrients, 13(2), 495.

+ Open protocol

+ Expand

Vitamin D Deficiency Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma samples were assayed for 25-hydroxyvitamin D₃ using a radioimmunoassay kit (DiaSorin, Stillwater, MN, USA), and vitamin D deficiency was defined as plasma levels of 25-hydroxyvitamin D₃ lower than 20 ng/mL.19 (link)

Park Y., Kim Y.S., Kang Y.A., Shin J.H., Oh Y.M., Seo J.B., Jung J.Y, & Lee S.D. (2016). Relationship between vitamin D-binding protein polymorphisms and blood vitamin D level in Korean patients with COPD. International Journal of Chronic Obstructive Pulmonary Disease, 11, 731-738.

+ Open protocol

+ Expand

Comprehensive Metabolic Profiling of Fasting Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were obtained after 12 h or more fasting. HbA1c from whole blood, plasma glucose concentrations, and serum concentrations of total and HDL-cholesterol and triglycerides were measured using an automatic analyzer (ZEUS 9.9; Takeda, Tokyo, Japan). Serum LDL cholesterol concentrations were calculated by the Freidman equation. Serum insulin concentrations were measured using a gamma counter (1470 Wizard; Perkin-Elmer, San Jose, CA, USA) with a radioimmunoassay kit (DiaSorin, Stillwater, MN, USA). Homeostasis model assessment estimates (HOMA) were used to estimate beta (B) cell capacity (HOMA-B) and insulin resistance (HOMA-IR). HOMA assessments were calculated as previously described [15 (link)]. Systolic (SBP) and diastolic blood pressure (DBP) were measured three times on the right arm at the same height as their heart in a sitting position with a 1 min interval between measurements; we used the averages of the three measurements of SBP and DBP. Serum C-reactive protein (CRP) was measured by ELISA kit.

Daily J.W., Yang H.J., Liu M., Kim M.J, & Park S. (2019). Subcutaneous fat mass is associated with genetic risk scores related to proinflammatory cytokine signaling and interact with physical activity in middle-aged obese adults. Nutrition & Metabolism, 16, 75.

+ Open protocol

+ Expand

Fasting Blood Analysis for Metabolic Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Venous blood samples were collected the morning after PSG following at least 8 h of fasting. Biochemical parameters of renal function and lipid profile, as well as glucose and C‐reactive protein (CRP) serum levels were measured using an automated analyser. Serum biochemical parameters were determined using a commercial radioimmunoassay kit and the manufacturer's specifications the day of blood sampling (DiaSorin, Stillwater, MN, USA).

Voulgaris A., Archontogeorgis K., Anevlavis S., Fanaridis M., Froudarakis M.E., Schiza S, & Steiropoulos P. (2023). Effect of compliance to continuous positive airway pressure on exacerbations, lung function and symptoms in patients with chronic obstructive pulmonary disease and obstructive sleep apnea (overlap syndrome). The Clinical Respiratory Journal, 17(3), 165-175.

+ Open protocol

+ Expand

Serum Biomarker Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum cholesterol, triglycerides, creatinine and urea were measured using an automatic analyser Integra 800 (Roche Diagnostic, Mannheim, Germany). Plasma renin activity was measured with a commercially available radioimmunoassay kit (DiaSorin, Stillwater, MN, USA).

Menendez-Castro C., Nitz D., Cordasic N., Jordan J., Bäuerle T., Fahlbusch F.B., Rascher W., Hilgers K.F, & Hartner A. (2018). Neonatal nephron loss during active nephrogenesis – detrimental impact with long-term renal consequences. Scientific Reports, 8, 4542.

+ Open protocol

+ Expand

Measuring Serum Vitamin D Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood specimens were collected after an 8-hour fast and were centrifuged to isolate the serum. The concentration of 25(OH)D, with equimolar measurements of 25(OH)D₂ and 25(OH)D₃, which is a marker of vitamin D status, was measured using a radioimmunoassay kit (DiaSorin, Stillwater, MN, USA) using the 1470 WIZARD gamma-Counter (PerkinElmer, Turku, Finland). The KCDC continuously monitored and checked 25(OH)D levels via internal and external quality control. Internal quality control, using the Westgard multi-rule control method, is performed to evaluate the precision and accuracy of an analytical system using mean, standard deviation (SD), and coefficient of variation (%) values. The coefficient of variation for the control material was <7.6% for the low level and <5.8% for the high level. External quality control was evaluated using the Vitamin D Quality Assessment Scheme, Vitamin D Metabolites Quality Assurance Program of the National Institute of Standards and Technology, Accuracy-based Vitamin D Survey of College of American Pathologists.17 The participants were divided into the following three categories according to their 25(OH)D concentration based on established definitions and practical guidelines: vitamin D deficient (<10 ng/mL), insufficient (10–19.99 ng/mL), and sufficient (≥20 ng/mL).18 (link),19 (link)

Kim S.Y., Lee M.H., Lim W.J., Kim S.I, & Lee Y.J. (2020). Associations of 25-Hydroxyvitamin D Levels and Arthritis with Sleep Duration: The Korean National Health and Nutrition Examination Survey 2008–2014. Nature and Science of Sleep, 12, 883-894.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!