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Mouse monoclonal antibodies against

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Mouse monoclonal antibodies are laboratory-produced antibodies that recognize and bind to specific target antigens. They are derived from a single B-cell clone and are highly specific for their target. These antibodies can be used as research tools in various applications such as immunoassays, immunohistochemistry, and Western blotting.

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Lab products found in correlation

Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions) Gapdh antibody, by Cell Signaling Technology (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Colchicine, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Revet aid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Dynamotm color flash sybr green qpcr kit, by Thermo Fisher Scientific (1 mentions) Hrp conjugated anti mouse igg antibody, by Abcam (1 mentions) Rhodamine b, by Merck Group (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Verapamil, by Merck Group (1 mentions) Lps from e coli o111 b4, by Merck Group (1 mentions) Dylight 488 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Rnase free recombinant dnase 1, by Roche (1 mentions) Dylight 649, by Jackson ImmunoResearch (1 mentions) Power sybr green master mix, by Thermo Fisher Scientific (1 mentions) Dispase 2, by Roche (1 mentions) Collagenase a, by Roche (1 mentions) Raw264.7 cell line, by American Type Culture Collection (1 mentions) Protector rnase inhibitor, by Roche (1 mentions)

3 protocols using mouse monoclonal antibodies against

1

Quantifying Oxidative DNA Damage in Cells

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Cells (1×105/ml) were plated with or without exposure to strain and fixed with formaldehyde (Wuhan Goodbio Technology Co., Ltd.). and incubated overnight at 4°C with mouse monoclonal antibodies against 8-OHdG (1:200; Abcam; cat. no. ab62623). The plates were washed in TBST and incubated with goat anti-mouse secondary antibodies labeled with FITC (1:1,000; Abcam; ab7064). The nuclei were stained with 4′,6-diamidino 2-phenylindole. The fluorescence-stained cells were observed under fluorescence microscopy (IX51).
LI B.S., GUO W.J., HONG L., LIU Y.D., LIU C., HONG S.S., WU D.B, & MIN J. (2016). Role of mechanical strain-activated PI3K/Akt signaling pathway in pelvic organ prolapse. Molecular Medicine Reports, 14(1), 243-253.
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2

Saikosaponin Compound Characterization

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The test compounds saikosaponin A (purity > 95%), C (purity > 95%) and D (purity > 95%) were purchased from Winherb Medical Technology (Shanghai, China). Colchicine, rhodamine B, verapamil, and 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RIPA buffer (1×), Pierce®BCA protein assay kit, and the Trizol® reagent were from Invitrogen (Carlsbad, CA, USA). The Revet Aid First Strand cDNA Synthesis kit and DyNAmoTM Color Flash SYBR® Green qPCR kit were obtained from Thermo Scientific (Rockford, IL, USA). Human Pgp and GAPDH primers were synthesized by Invitrogen (Guangzhou, China). OCT2-pCMV6-AC-GFP, Mrp2-pCMV6-AC-GFP, ABCB1-pCMV6-AC-GFP, and pCMV6-AC-GFP plasmids and Mega Tran1.0 transfection reagent were purchased from OriGene Technologies Company (Maryland, USA). The mouse monoclonal antibodies against organic cation transporter 2 (Oct2), Pgp, Mrp1, and Mrp2 and anti-mouse IgG HRP-conjugated antibody were purchased from Abcam (Cambridge, UK). The GAPDH antibody was obtained from Cell Signaling Technology (Danvers, MA, USA). All other chemicals used in the present study were commercially available and of analytical reagent grade.
Feng L., Liu L., Zhao Y, & Zhao R. (2017). Saikosaponins A, C and D enhance liver-targeting effects of anticancer drugs by modulating drug transporters. Oncotarget, 8(66), 110092-110102.
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3

Inducible Nitric Oxide Synthase Quantification

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Rabbit polyclonal antibodies to iNOS and Mouse monoclonal antibodies against iNOS were purchased from Abcam (Cambridge, UK). Rabbit polyclonal antibodies against PLC-β2 (sc-206) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). An affinity-purified goat polyclonal antibody against carbonic anhydrase 4 (CA4, AF2414) was purchased from R&D Systems. An affinity-purified rabbit polyclonal antibody against P2X3 was purchased from Sigma-Aldrich (AB5895). DyLight 649 (or DyLight 488)-conjugated donkey anti-rabbit, anti-mouse, or anti-goat antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). Collagenase A and dispase II were from Roche Applied Science (Indianapolis, IN). LPS (from E. coli O111:B4) was purchased from Sigma (St. Louis, MO). Nitric Oxide Assay Kit for NO determination was purchased from R&D Systems (Minneapolis, USA). Superscript III Reverse Transcriptase Reagent Kit was purchased from Invitrogen. Protector RNase Inhibitor and recombinant RNase-free DNase I were from Roche Applied Science. PowerUP SYBR Green Master Mix or Power SYBR Green master Mix for real-time PCR was obtained from Thermo Fisher (Waltham, MA, USA). RAW 264.7 cell line was purchased from American Type Culture Collection (ATCC; Manassas, VA).
Leonetti J.P., Machy P., Degols G., Lebleu B, & Leserman L. (1990). Antibody-targeted liposomes containing oligodeoxyribonucleotides complementary to viral RNA selectively inhibit viral replication.. Proceedings of the National Academy of Sciences of the United States of America, 87(7), 2448-2451.
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