Ca125 2

Serum for the analysis of CA125 (U/mL), HE4 (/L), creatinine (μmol/L) and pro-BNP (ng/L) were collected at the time of enrollment prior to surgery, centrifuged and stored in 4 °C before being shipped, according to standardized procedures, to Sahlgrenska University hospital as previously described [2] . The coded samples were analyzed at an accredited central laboratory (ISO 15189) Sahlgrenska University hospital in three separate batches. CA125, HE4, NT-pro-BNP and creatinine were analyzed using the Elecsys HE4 and Elecsys CA125 II with the electrochemiluminescence (ECLIA) technique (Cobas 6,000 and 8,000, RocheDiagnostics Scandinavia, Stockholm, Sweden) [2] . Recommended cut-offs according to the manufacturer were used in the study; CA125 > 35 U/mL, HE4 pre-M > 70 pmol/L, HE4 post-M > 140 pmol/L [23] . Glomerular filtration rate (eGFR) was estimated using creatinine as a measure of renal function using the revised Lund-Malmö equation [24] . Continuous levels of eGFR were used as a measure for impaired kidney function and continuous levels of NT-pro-BNP were used as a measure of heart disease in the multivariable analyses.