Carbo sorb e

Manufactured by PerkinElmer

Sourced in United States

Carbo-Sorb® E is a laboratory equipment product designed for carbon dioxide absorption. It is a high-capacity, granular carbon dioxide absorbent material.

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Lab products found in correlation

Permafluor e, by PerkinElmer (6 mentions) Hionic fluor, by PerkinElmer (2 mentions) Ethanolamine, by Merck Group (1 mentions) Ultima flo m, by PerkinElmer (1 mentions) Beckman ls 6500, by Beckman Coulter (1 mentions) Methanol, by Avantor (1 mentions) β glucuronidase from e coli, by Merck Group (1 mentions) Pico fluor 40, by PerkinElmer (1 mentions) Ls6000ta, by Beckman Coulter (1 mentions) Tri carb 2800tr, by PerkinElmer (1 mentions) Sample oxidizer, by PerkinElmer (1 mentions) 1200 series hplc, by Agilent Technologies (1 mentions) Ramona star, by Raytest (1 mentions)

8 protocols using carbo sorb e

Synthesis and Characterization of [14C]PPI-1011

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PPI-1011 (1-O-hexadecyl-2-O-cis-4, 7, 10, 13, 16, 19-docosahexaenoyl-3-O-lipoyl glycerol) was synthesized at Laxai Life Sciences Pvt Ltd. using a previously described synthetic route (Wood et al., 2011a ). Structure was confirmed by mass spectrometry and NMR analysis. [¹⁴C]PPI-1011 was synthesized by RTI International using [¹⁴C]sodium cyanide to introduce a single [¹⁴C] label onto the first carbon of palmitoyl alcohol, which was linked to the sn1 position of the glycerol backbone. [¹⁴C]PPI-1011 had a specific activity of 70.2 μCi/mg and a radiochemical purity of 95.9%. Handling of this material was in accordance with US Nuclear Regulatory Commission (NRC), Pennsylvania Bureau of Radiation Protection regulations, Frontage QWBA final study protocol, and all applicable Frontage Standard Operating Procedures (SOP).
Ethanolamine and 2-methoxyEthanolamine were purchased from Sigma. Carbo-Sorb^® E, PermaFluor^® E+, Ultima Gold™, Hionic-Fluor, and Ultima-Flo™ M liquid scintillation cocktails were obtained from Perkin Elmer Life Sciences. Stopflow AQ liquid scintillation cocktails were purchased from AIM Research Company. Solvents used for chromatographic analysis were HPLC or ACS reagent grade and purchased from Fisher. All other reagents were of analytical or ACS reagent grade.

Smith T., Knudsen K.J, & Ritchie S.A. (2022). Pharmacokinetics, Mass Balance, Excretion, and Tissue Distribution of Plasmalogen Precursor PPI-1011. Frontiers in Cell and Developmental Biology, 10, 867138.

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Tracing Glyphosate Uptake and Translocation in Amaranthus Hybrids

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Amaranthus hybridus plants of the GRH and GSH populations were treated with ¹⁴C-glyphosate [glycine-2-¹⁴C] (specific activity 273.8 MBq mmol⁻¹, American Radiolabeled Chemicals, Inc., Saint Louis, MO, USA) + commercial glyphosate. The final glyphosate concentration corresponded to 300 g ae ha⁻¹ in 200 L ha⁻¹ with a specific activity of 0.834 kBq µL⁻¹. GRH and GSH plants at the 4-leaf growth stage were treated with a 1-µL drop (0.834 KBq plant⁻¹) placed with a micropipette (LabMate) on the adaxial surface of the second leaf. The plants were handled according to Alcántara-de la Cruz et al. [13 (link)] at 12, 24, 48, 72, and 96 h after treatment (HAT) (five plants per population at a time were evaluated in a completely random design). Samples (section of divided plants), once stored into cellulose cones and dried at 60 °C, were combusted in a biological oxidizer (TriCarb 307, Packard Instrument Co., Downers Grove, IL, USA). The ¹⁴CO₂ released by combustion was trapped and mixed with 18 mL of Carbo-Sorb® E and Permaf1uor® (Perkin-Elmer, Groningen, Netherlands) (1:1 v/v). Radioactivity of ¹⁴C was quantified by liquid scintillation spectrometry in a scintillation counter (Beckman LS-6500, Beckman Coulter Inc., Fullerton, CA, USA). Radioactive data was used to calculate the percentages of ¹⁴C recovered, absorbed and translocated.

García M.J., Palma-Bautista C., Rojano-Delgado A.M., Bracamonte E., Portugal J., Alcántara-de la Cruz R, & De Prado R. (2019). The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus. International Journal of Molecular Sciences, 20(10), 2396.

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Radiolabeled HMB Synthesis and Characterization

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HMB (CAS # 131-57-7, purity 98%, lot # 05928TH) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO) and the identity was confirmed by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). [¹⁴C]HMB (lot# 433106–0748-A-20090223-JK, specific activity 74.8 mCi/mmol, 1mCi/mL, label on the unsubstituted phenyl ring) was from Moravek Biochemicals, Inc. (Brea, CA). The radiochemical purity of [¹⁴C]HMB was determined to be 99.1% by high performance liquid chromatography (HPLC) and liquid scintillation spectrometry (LSS).
Ultima Gold scintillation cocktail was purchased from Packard Instrument Company, Inc. (Meriden, CT). Alkamuls EL-620 was purchased from Rhodia (Cranbury, NJ). Methanol was purchased from VWR International, LLC (Radnor, PA). DHB, 2,2′-DHMB, THB, β-Glucuronidase from E. coli and H. pomatia were purchased from Sigma-Aldrich Company, Inc (St. Louis, MO). 2,4- DHMB was custom synthesized by Richman Chemical Custom Solutions (Gwynedd, PA). Carbo-Sorb E and Permafluor E+ were purchased from Perkin Elmer (Shelton, CT). Heparin was from Abraxis Pharmaceutical Products (Schaumburg, IL) and Euthasol was from Delmarva Laboratories, Inc. (Midlothian, VA). Chlorhexidine 2% was purchased from VetOne (Meridian, ID). All other reagents were purchased from commercial sources.

Mutlu E., Garner C.E., Wegerski C.J., McDonald J.D., McIntyre B.S., Doyle-Eisele M, & Waidyanatha S. (2019). Metabolism and Disposition of 2-Hydroxy-4-Methoxybenzophenone, a Sunscreen Ingredient, in Harlan Sprague Dawley Rats and B6C3F1/N Mice; a Species and Route Comparison. Xenobiotica; the fate of foreign compounds in biological systems, 50(6), 689-704.

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Radiolabeled Carbohydrate Synthesis Protocol

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D-[1-¹⁴C] allose (37.2 mM 3% ethanol solution, 1.96 GBq/mmol) and D-[1-¹⁴C] glucose (0.654 mM 0.3% EtOH solution, 11.3 GBq/mmol) were obtained from Amersham Biosciences UK Ltd (Little Chalfont, Buckinghamshire, UK). Cold D-psicose, D-allose, and L-rhamnose isomerase immobilized beads were supplied by the Rare Sugar Research Center of Kagawa University (Miki, Kagawa, Japan). Carbo-Sorb^® E and Permafluor^® E⁺ were from PerkinElmer, Inc., (Waltham, MA, USA).

Tsukamoto I., Hossain A., Yamaguchi F., Hirata Y., Dong Y., Kamitori K., Sui L., Nonaka M., Ueno M., Nishimoto K., Suda H., Morimoto K., Shimonishi T., Saito M., Song T., Konishi R, & Tokuda M. (2014). Intestinal absorption, organ distribution, and urinary excretion of the rare sugar D-psicose. Drug Design, Development and Therapy, 8, 1955-1964.

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Synthesis and Characterization of 14C-Amenamevir

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¹⁴C-amenamevir (Fig. 1, specific activity 2.23 MBq/mg and radiochemical purity > 99%) was synthesized at EaglePicher Pharmaceutical Services (Lenexa, KS, USA). Unlabeled amenamevir, its solid dispersion (amenamevir:hydroxypropylmethylcellulose 2910 [TC-5 E] = 1:3), internal standard (AS1709867-00, D₆-amenamevir), and metabolites R1 and R5 were supplied by Astellas Pharma (Tokyo, Japan). All organic solvents for high-performance liquid chromatography (HPLC) were of HPLC grade. Carbon dioxide absorbent (Carbo-Sorb E) and scintillation cocktails (Hionic-Fluor, PermaFluor E⁺, and Pico-Fluor 40) were purchased from PerkinElmer (Waltham, MA, USA). All other reagents were of analytical grade.

Ohtsu Y., Susaki Y, & Noguchi K. (2018). Absorption, Distribution, Metabolism, and Excretion of the Novel Helicase-Primase Inhibitor, Amenamevir (ASP2151), in Rodents. European Journal of Drug Metabolism and Pharmacokinetics, 43(6), 693-706.

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Oleic Acid Metabolism in Mice

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ICR male mice at 20 weeks of age were divided into two groups on the basis of body weight in the morning after 16‐h fast and then orally administered with or without SKL‐14959 (100 mg kg⁻¹ body weight) prepared with 0.5% sodium carboxymethylcellulose solution 15 min prior to oral administration of [¹⁴C]‐oleic acid (18.5 MBq g⁻¹). The mice were immediately released into an occlusive metabolic cage Metabolica from Yamagen Co., Ltd. (Tokyo, Japan) after the challenge and then euthanized under ether anaesthesia at 60 and 120 min. Blood samples were collected from the inferior vena cava, and epididymal adipose tissue, soleus muscles and liver were removed and weighed. Sections of all samples including blood were oxidized using a combustion system (ASC‐113, Aloka), and the radioactivity in the absorbent (Carbo‐Sorb E, PerkinElmer), which absorbed yields of ¹⁴C‐carbon dioxide, was measured using a liquid scintillation counter (LS6000TA, Beckman). Those data were expressed as a concentration of each whole tissue.

Nakamura T., Tanimoto H., Mizuno Y., Okamoto M., Takeuchi M., Tsubamoto Y, & Noda H. (2018). Gastric inhibitory polypeptide receptor antagonist, SKL‐14959, suppressed body weight gain on diet‐induced obesity mice. Obesity Science & Practice, 4(2), 194-203.

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Quantifying Plant and Soil Carbon

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Dry plant and soil material was ground and ¹⁴C in samples quantified by liquid scintillation counting (LSC) of ¹⁴CO₂ produced by dry combustion (Packard 307 sample oxidizer; 6 ml Carbosorb E mixed with 12 ml Permafluor E, Perkin Elmer).
Soil microbial C was determined by chloroform fumigation‐extraction (Vance, Brookes, & Jenkinson, 1987), with some modifications (Supporting Information). For L. alpina, ¹⁴C in microbial extracts was below the detection limit, most likely because of the lower amount of ¹⁴C applied during pulse‐labelling.

Ferrari A., Hagedorn F, & Niklaus P.A. (2018). Disentangling effects of air and soil temperature on C allocation in cold environments: A 14C pulse‐labelling study with two plant species. Ecology and Evolution, 8(16), 7778-7789.

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Radioactivity Assessment of Solid and Liquid Samples

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Total radioactivity of the samples was assessed using a liquid scintillation counter (Tri-Carb 2800TR, Perkin Elmer). Sample aliquots were mixed with scintillation cocktail prior to the measurements as described above. For analysis of the radioactivity accumulated in the solids, the biomass fractions of the samples were combusted for 1.5 min in a 307 Perkin Elmer Sample Oxidizer. The resulting 14 CO 2 was absorbed by Carbo-Sorb® E (Perkin Elmer) and LSC cocktail (Permafluor® E+, Perkin Elmer) was added. The radioactivity was then assessed by liquid scintillation counting. Radioactive supernatant samples were analyzed using a HPLC 1200 Series (Agilent Technologies) including a diode array detector (DAD) coupled to a subsequent liquid scintillation counter (Ramona Star, Raytest). The DAD signal was recorded at 285 nm (4 nm bandwidth), which is close to the maximum of an absorption peak between 250 and 300 nm in the absorption spectra of both SMX and SDZ. 41, 42 An identical column and gradient program and identical eluent mixtures and injection volumes for chromatographic separation as described for the LC-HRMS/MS measurements above were used.

Achermann S., Bianco V., Mansfeldt C.B., Vogler B., Kolvenbach B.A., Corvini P.F.X, & Fenner K. (2018). Biotransformation of Sulfonamide Antibiotics in Activated Sludge: The Formation of Pterin-Conjugates Leads to Sustained Risk. Environmental science & technology, 52(11).

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