TUNEL positive cells in liver sections were stained with the commercial kit DeadEnd Colorimetric System (Promega, Madison, WI). Under light microscopy, the numbers of TUNEL positive cells in 10 randomly selected high power fields (200× magnification) were counted per liver section.
Deadend colorimetric system
Manufactured by Promega
Sourced in United States
The DeadEnd Colorimetric System is a laboratory equipment product designed to detect and quantify DNA fragments, particularly those associated with apoptosis or programmed cell death. The system provides a colorimetric assay for the identification and measurement of DNA fragmentation, which is a hallmark of apoptosis.
Automatically generated - may contain errors
10 protocols using deadend colorimetric system
1
Quantifying Liver Cell Death
2
Quantifying Apoptotic Cells in Liver
3
Placental Apoptosis Assessment by TUNEL
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Apoptosis in placentas was assessed by a TUNEL assay as described previously [24 (link)]. Placental sections were deparaffinized and placed on Fisherbrand Superfrost Plus® slides. Detection of cells undergoing apoptosis was performed using the kit DeadEnd Colorimetric System® Promega TUNEL, according to the manufacturer's protocol. The apoptotic index was calculated based on the proportion of TUNEL stained cells observed in 10 fields (40x).
4
Quantitative Histological Analysis of Breast Tumors
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5-μm sections of FFPE tissue were used for H&E staining, IHC with Ki67 antibody (Biocare Medical), TUNEL (DeadEnd Colorimetric System; Promega), or CD31 (BD Biosciences). Proportions of positively stained cells were counted in three random microscopic fields (400× magnification) in each specimen using ImageJ. Quantification of IHC for Ki67 and TUNEL was performed using the NIH ImageJ plugin IHC Image Analysis Toolbox. Quantification of IHC for CD31 was performed using NIH ImageJ as previously described (Owens et al., 2010 (link)). IHC images for human breast tumors were obtained from THPA (Uhlén et al., 2015 (link)), and images from four representative breast tumors (two ductal and two lobular adenocarcinomas) were examined and analyzed by a collaborating board-certified pathologist with expertise in breast cancer (J. Marotti).
5
Placental Apoptosis Assessment by TUNEL
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Apoptosis in placentas was assessed by a TUNEL assay as described earlier [28 (link)]. Placental sections were deparaffinized and placed on Fisherbrand Superfrost Plus® slides. Detection of cells undergoing apoptosis was performed using the kit DeadEnd Colorimetric System® Promega TUNEL, according to the manufacturer’s protocol. The apoptotic index was calculated based on the proportion of TUNEL stained cells observed in 10 fields (40x). The test was performed in a blinded fashion, for this samples were assigned a code which was revealed once all results were available.
6
Quantifying Apoptotic Cells in Liver
7
Quantification of Cell Proliferation
8
TUNEL Assay for Apoptosis in Diabetes
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The TUNEL assay was performed to investigate the fragmentation of DNA during apoptosis. DeadEndTM colorimetric system (Promega, Madison, WI, USA) was employed to identify apoptotic cells in the liver tissue of diabetic rats.
9
TUNEL Assay for Apoptosis Detection
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TUNEL assays were performed to detect DNA fragmentation during apoptosis in paraffin-embedded tissue sections. TUNEL assays involve fixation and permeabilization of the tissue, followed by in addition of TUNEL reagents. Apoptotic cells were analyzed using the DeadEndTM colorimetric system (Promega, Madison, WI, USA).
10
Detecting Apoptosis in Diabetic Rats
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TUNEL assay was used to determine DNA fragmentation during apoptosis. The DeadEndTM colorimetric system (Promega, Madison, WI, USA) was used to detect apoptotic cells in the kidney tissue of diabetic rats.
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