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22 protocols using stmisola

1

Selective Stimulation of Zygomatic Muscle

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With the patient in upright sitting position, two 60 × 40 mm oval surface electrodes (Krauth+Timmermann, Hamburg, Germany) were placed in correspondence with the paralyzed ZYG as close as possible to the mouth corner in order to prevent unspecific stimulation of other facial muscles (Figure 1). STMIsola (BIOPAC Systems, Inc., Essen, Germany) connected to the PowerLab (ADInstruments, Sydney, Australia) system was used to deliver the stimulation. The upper electrode was always the cathode and the lower the anode. The muscle was stimulated bipolarly, without the use of a reference electrode to selectively limit the electrical field to the area of the ZYG.
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2

Electrodermal Responses to Visual Stimuli

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A Dell OptiPlex equipped with MATLAB software and Psychophysics Toolbox extensions (Brainard, 1997 (link)) was used to present the stimuli on a Dell P2717H monitor. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Eye position was monitored using an EyeLink 1000 Plus desktop-mounted eye tracker (SR Research). Head position was maintained using an adjustable chin rest (SR Research). Paired electrodes (EL500, BioPac Systems, Inc.) were attached to the left forearm of each participant, and electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems), which was controlled by custom Matlab scripts.
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3

Multimodal Pain Perception Experiment

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A Dell OptiPlex 7040 (Dell, Round Rock, TX, USA) equipped with Matlab software (Mathworks, Natick, MA, USA) and Psychophysics Toolbox extensions (Brainard, 1997 (link)) was used to present the stimuli on a Dell P2717H monitor. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Paired electrodes (EL500, BioPac Systems, Inc., Goleta, CA, USA) were attached to the left forearm of each participant, and electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems), which was controlled by custom Matlab scripts. Eye-tracking was conducted using the EyeLink 1000 Plus system (SR Research Ltd., Ottawa, Ontario, Canada), and head position was maintained using a manufacturer-provided chin rest (SR Research Ltd.).
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4

Multimodal Sensory Stimulation Protocol

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A Dell OptiPlex equipped with Matlab software and Psychophysics Toolbox extensions (Brainard, 1997) was used to present the stimuli on a Dell P2717H monitor. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Eye position was monitored using an EyeLink 1000-plus desktop mount eye tracker (SR Research). Head position was maintained using an adjustable chin and forehead rest (SR Research). Electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems) using paired electrodes (EL500, BioPac Systems).
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5

Visual Stimulus Presentation and Eye Tracking

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A Dell OptiPlex equipped with Matlab software and Psychophysics Toolbox extensions (Brainard, 1997 (link)) was used to present the stimuli on a Dell P2717H monitor. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Eye position was monitored using an EyeLink 1000-plus desktop mount eye tracker sampling at 1000 Hz. Head position was maintained using an adjustable chin and forehead rest (SR Research). Electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems).
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6

Multimodal Neurophysiological Assessment of Aversive Stimuli Processing

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During the initial in-lab visit, all tasks were completed on a Dell OptiPlex 7040 computer (Dell, Round Rock, TX, USA) equipped with Matlab software (Mathworks, Natick, MA, USA), and Psychophysics Toolbox extensions (Brainard, 1997 ). Stimuli were presented on a Dell P2717H monitor. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Paired electrodes (BioPac Systems, Inc., Goleta, CA, USA) were attached to the left forearm of each participant, and electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems), which were controlled by custom Matlab scripts.
For the fMRI portion of the experiment, stimulus presentation was controlled by an Invivo SensaVue display system. The eye-to-screen distance was approximately 125 cm. Key responses were entered using two Cedrus Lumina two-button response pads. MRI-compatible electrodes (BioPac Systems) were attached to the left ankle of each participant, and electric shocks were delivered through an STM100C controlled by an MP160 system (BioPac Systems) triggered by custom Matlab scripts via parallel port interface.
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7

Neuromuscular Electrical Stimulation Protocol

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Two constant-current electric stimulators (STMISOLA, Biopac, USA) controlled by an USB-6343 interface (NI, USA) and custom-written program in Labview (NI, USA) were used to deliver the stimuli via low impedance carbon adhesive surface electrodes (area = 2cm2 for each electrode). One stimulator was used to stimulate the fibular nerve at the fibular head and the other was used to stimulate the tibial nerve at the popliteal fossa [13 (link), 35 (link)]. Stimulation locations were determined both at rest and during contractions to take into account changes in muscle geometry during muscle contraction. Electrical stimulation parameters were determined during the familiarization session and confirmed during each experimental session before performing the fatiguing contraction.
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8

Painful Electrical Stimuli Decision-Making

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While participants were performing a decision-making task (see below) they received transcutaneous electrical stimuli using a constant voltage isolated linear stimulator (STMISOLA, BIOPAC Systems, Inc.). Stimuli were applied to the participants’ non-dominant volar forearm using disposable surface electrodes. Participants’ sensitivity to the electrocutaneous stimuli was assessed and stimulus intensities in the decision-making task individually adjusted to induce a strongly painful and/or unpleasant sensation (see below). Each stimulus in the task lasted two seconds, consisting of 100 two-millisecond pulses with 18 milliseconds inter-pulse intervals.
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9

Multimodal Neurophysiological Assessment of Aversive Stimuli Processing

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During the initial in-lab visit, all tasks were completed on a Dell OptiPlex 7040 computer (Dell, Round Rock, TX, USA) equipped with Matlab software (Mathworks, Natick, MA, USA), and Psychophysics Toolbox extensions (Brainard, 1997 ). Stimuli were presented on a Dell P2717H monitor. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Paired electrodes (BioPac Systems, Inc., Goleta, CA, USA) were attached to the left forearm of each participant, and electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems), which were controlled by custom Matlab scripts.
For the fMRI portion of the experiment, stimulus presentation was controlled by an Invivo SensaVue display system. The eye-to-screen distance was approximately 125 cm. Key responses were entered using two Cedrus Lumina two-button response pads. MRI-compatible electrodes (BioPac Systems) were attached to the left ankle of each participant, and electric shocks were delivered through an STM100C controlled by an MP160 system (BioPac Systems) triggered by custom Matlab scripts via parallel port interface.
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10

Electric Shock Delivery System for Experiments

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A Dell OptiPlex 7040 (Dell, Round Rock, TX, USA) equipped with Matlab software (Mathworks, Natick, MA, USA) and Psychophysics Toolbox extensions (Brainard, 1997 (link)) was used to present the stimuli on a Dell P2717H monitor. Responses were entered using a standard US-layout keyboard. The participants viewed the monitor from a distance of approximately 70 cm in a dimly lit room. Paired electrodes (EL500, BioPac Systems, Inc., Goleta, CA, USA) were attached to the left forearm of each participant, and electric shocks were delivered through an isolated linear stimulator under the constant current setting (STMISOLA, BioPac Systems), which was controlled by custom Matlab scripts.
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