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Clarus 500 mass spectrometer

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The Clarus 500 is a mass spectrometer designed for analytical applications. It utilizes electron ionization (EI) and chemical ionization (CI) techniques to ionize and analyze samples. The instrument is capable of performing full-scan and selected ion monitoring (SIM) analyses. The Clarus 500 provides accurate mass measurements and quantitative performance for a wide range of analytes.

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Lab products found in correlation

Clarus 500 gc, by PerkinElmer (4 mentions) Clarus 680 gas chromatograph, by PerkinElmer (2 mentions) Split splitless injector, by PerkinElmer (2 mentions) Dbwax, by Restek (2 mentions) Dvb car pdms, by Merck Group (2 mentions) Lambda 35 uv vis spectrophotometer, by PerkinElmer (1 mentions) Elite 5ms capillary column, by PerkinElmer (1 mentions) Stabilwax capillary column, by Restek (1 mentions)

8 protocols using clarus 500 mass spectrometer

1

Photocatalytic Degradation of BPA

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The photocatalytic activity of codoped photocatalysts were examined for the degradation BPA under solar light followed by adsorption-desorption equilibrium in dark for 20 mins. During the solar light irradiation experiments, stirring was maintained by magnetic stirrer to manage the solution homogeneous. A fixed amount of sample was withdrawn at different time intervals over 140 min and placed into different syringes and filters. The BPA concentration was determined using a UV-vis spectrophotometer (Perkin Elmer Lambda 35UV-Vis spectrophotometer) at λmax = 277 nm followed by centrifugation for the separation of catalyst. The percentage photodegradation of BPA was calculated using the equation (4): PercentagephotodegradationofBPA=Co−CCo×100 Where, Co is the initial concentration of BPA and C is the concentration of BPA at time ‘t’.
For complete mineralization of the BPA solution, the total organic carbon (TOC) content was estimated using Schimadzu model TOC-VCPH Total Organic Carbon Analyzer.
Gas chromatography- mass spectroscopy (GC-MS) of the photo-degraded BPA wastewater was carried out to identify the various intermediates produced during photocatalytic degradation of BPA in wastewater. The GC-MS analysis was carried out by Perkin Elmer Clarus 500 GC coupled with a Perkin Elmer Clarus 500 mass spectrometer.
Garg A., Singhania T., Singh A., Sharma S., Rani S., Neogy A., Yadav S.R., Sangal V.K, & Garg N. (2019). Photocatalytic Degradation of Bisphenol-A using N, Co Codoped TiO2 Catalyst under Solar Light. Scientific Reports, 9, 765.
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2

GC-FID and GC-MS Analysis of Samples

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GC/FID analysis was carried out using a Perkin Elmer model 8500 GC (Perkin Elmer Italia Spa, Milano, Italy) equipped with a 30 m × 0.32 mm i.d., Elite-5MS capillary column (0.32 μm film thickness). The sample (0.5 μL) was injected in the “split” mode (1:30), with a column temperature program of 40 °C for 5 min, then increased to 260 °C at 4 °C/min and finally held at that temperature for 10 min. Injector and detector were set at 230 °C and 280 °C, respectively; the carrier gas was He with a head pressure of 12.0 psi.
GC/MS analysis was carried out using a Perkin Elmer Clarus 500 GC equipped with a Clarus 500 mass spectrometer, using the same capillary column and chromatographic conditions as for the GC/FID analysis. Mass spectra were acquired over the 40–500 amu range at 1 scan/sec with ionizing electron energy 70 eV, ion source 230 °C. The transfer line was set at 270 °C, while the carrier gas was He at 1.0 mL/min.
Tava A., Biazzi E., Ronga D, & Avato P. (2020). Identification of the Volatile Components of Galium verum L. and Cruciata leavipes Opiz from the Western Italian Alps. Molecules, 25(10), 2333.
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3

Grape Volatile Profiling via GC-MS

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Free volatile aroma precursors have been analyzed at Scuola Superiore Sant’Anna (Pisa, Italy) employing gas chromatography coupled with mass spectrometry (GC-MS) equipment. The solid-phase micro extraction (SPME) technique has been used to analyze the head space of grape samples, applying an untargeted analytical approach to achieve grape berry volatile profiling. Grapes were homogenized with 1 M NaCl buffer solution (1:1 ratio in weight) by using an UltraTurrax (Mod. T25, IKA) and thawed at 15 °C for 15 min. 10 g of sample were transferred to a 20 mL glass crimp vial for headspace analysis and sealed. The vials were incubated under agitation for 45 min at 40 °C, and volatiles were sampled using an SPME fiber (50/30 µm, DVB/CAR/PDMS, 1 cm long; Supelco, Bellefonte, PA, USA). Sample analysis, as well as compound identification and quantification, have been performed according to Modesti et al. (2023) [111 (link)]. A Clarus 680 gas chromatograph equipped with a split/splitless injector (PerkinElmer®, Waltham, MA, USA) was used for the analysis. Volatiles were separated on a fused silica capillary column (DBWax, 60 m, 0.32 mm ID, 0.25 µm film thickness; Restek, Bellefonte, PA, USA), using helium as carrier gas with a flow rate of 1 mL/min. Compounds were identified using a mass spectrometer (Clarus 500 mass spectrometer, PerkinElmer®, Waltham, MA, USA) coupled to the GC.
Rodrigues M., Forestan C., Ravazzolo L., Hugueney P., Baltenweck R., Rasori A., Cardillo V., Carraro P., Malagoli M., Brizzolara S., Quaggiotti S., Porro D., Meggio F., Bonghi C., Battista F, & Ruperti B. (2023). Metabolic and Molecular Rearrangements of Sauvignon Blanc (Vitis vinifera L.) Berries in Response to Foliar Applications of Specific Dry Yeast. Plants, 12(19), 3423.
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4

GC-MS Analysis of C. sinensis Bioactive Compounds

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The recommended standard methods of Sofowora20
and Trease and Evans21
for quality screening of phenolics, alkaloids, flavonoids, steroids,
saponins, and cardiac glycosides were adopted.
The GC-MS was used to characterize the bioactive constituents in this study.
Briefly, the ethanol extract of the C. sinensis leaves, which
showed high antibacterial activity, was analyzed using a Clarus 500 Mass
Spectrometer (PerkinElmer, Waltham, MA, USA). It consists of a mass spectral
library (Mass Hunter-Library-NIST14.LIB GC-MS) that was used for the
identification of components in the GC/MS analysis. Its detectable mass ranges
from 35 to 500 m/z, while its ion source and interface temperature were 200°C
and 250°C, respectively. The start and end times (2.50 min and 47.14 min,
respectively), column oven temperatures (40°C), and injection temperatures
(25°C) were used. Injection mode was split and flow control mode was set at a
pressure of 100 kPa. Total flow was 13.9 ml/min while column flow was
1.78 ml/min with a linear velocity of 48.1 cm/s. Purge flow was kept at
3.0 ml/min and a split ratio of 5.1. The oven temperature was programmed first
at 40°C for 5 min with an increase of 5°C min−1 (link) to 80°C, then
5°C min−1 (link) to 300°C for 5 min.
Hope O., Bright I.E, & Alagbonsi A.I. (2022). GC-MS biocomponents characterization and antibacterial potency of ethanolic crude extracts of Camellia sinensis. SAGE Open Medicine, 10, 20503121221116859.
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5

GC/MS Analysis of n-Hexane Extract Bioactivity

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Constituents of n-hexane extract of C. macrophylla leaves showing higher bioactivity were analyzed by using GC/MS on a Clarus 500 Mass Spectrometer (PerkinElmer, Waltham, Massachusetts, USA) whose detectable mass range was set at 35–500 m/z. The ion source and interface temperatures were 200 °C and 250 °C, respectively. The start and end times were 2.50 min and 47.14 min, respectively. The column oven temp. was 40 °C whereas the injection temp. was 25 °C. Injection mode was split and flow control mode was set at a pressure of 100 kPa. Total flow was 13.9 mL/min while column flow was 1.78 mL/min with a linear velocity of 48.1 cm/sec. Purge flow was kept at 3.0 mL/min and a split ratio of 5.1. The oven temperature was programmed first at 40 °C for 5 min with an increase of 5 °C min−1 to 80 °C, then 5 °C min-1 to 300 °C for 5 min. The mass spectral library consulted for GC/MS analysis for the identification of components in our study was NIST14.lib. This part of the research was conducted at the Thermal Energy Research Lab., National University of Sciences and Technology, Islamabad, Pakistan.
Akbar M., Ali U., Khalil T., Iqbal M.S., Amin A., Naeem R., Nazir A., Waqas H.M., Aslam Z., Jafri F.I., Aslam N, & Chohan S.A. (2020). Cornus macrophylla, the Antibacterial Activity of Organic Leaf Extracts and the Characterization of the More Lipophilic Components by GC/MS. Molecules, 25(10), 2395.
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6

Grape Volatile Profiling via GC-MS

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Free volatile aroma precursors have been analyzed at Scuola Superiore Sant’Anna (Pisa, Italy) employing gas chromatography coupled with mass spectrometry (GC-MS) equipment. The solid-phase micro extraction (SPME) technique has been used to analyze the head space of grape samples, applying an untargeted analytical approach to achieve grape berry volatile profiling. Grapes were homogenized with 1 M NaCl buffer solution (1:1 ratio in weight) by using an UltraTurrax (Mod. T25, IKA) and thawed at 15 °C for 15 min. 10 g of sample were transferred to a 20 mL glass crimp vial for headspace analysis and sealed. The vials were incubated under agitation for 45 min at 40 °C, and volatiles were sampled using an SPME fiber (50/30 µm, DVB/CAR/PDMS, 1 cm long; Supelco, Bellefonte, PA, USA). Sample analysis, as well as compound identification and quantification, have been performed according to Modesti et al. (2023) [111 (link)]. A Clarus 680 gas chromatograph equipped with a split/splitless injector (PerkinElmer®, Waltham, MA, USA) was used for the analysis. Volatiles were separated on a fused silica capillary column (DBWax, 60 m, 0.32 mm ID, 0.25 µm film thickness; Restek, Bellefonte, PA, USA), using helium as carrier gas with a flow rate of 1 mL/min. Compounds were identified using a mass spectrometer (Clarus 500 mass spectrometer, PerkinElmer®, Waltham, MA, USA) coupled to the GC.
Rodrigues M., Forestan C., Ravazzolo L., Hugueney P., Baltenweck R., Rasori A., Cardillo V., Carraro P., Malagoli M., Brizzolara S., Quaggiotti S., Porro D., Meggio F., Bonghi C., Battista F, & Ruperti B. (2023). Metabolic and Molecular Rearrangements of Sauvignon Blanc (Vitis vinifera L.) Berries in Response to Foliar Applications of Specific Dry Yeast. Plants, 12(19), 3423.
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7

GC-MS Analysis of Essential Oils

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GC-MS analyses were carried out using a Perkin Elmer Clarus 500 GC equipped with a flame ionization detector and coupled with a Clarus 500 mass spectrometer. A Stabilwax capillary column (Restek, Bellefonte, PA, USA) was used with helium as carrier gas (1.0 mL/min). GC oven temperature was kept at 60°C for 5 min and programmed to 220°C at a rate of 5°C/min, and kept constant at 220°C for 30 min. Mass spectra were acquired over 40–500 amu with ionizing electron energy 70 eV. In all, 1 μL of the EO was diluted in 1 mL of methanol and 1 μL of the solution was injected into the GC injector at 280°C. The identification of compounds of EOs was performed by comparing mass spectra with those reported in Nist and Wiley libraries. Linear retention indices were calculated after injection of C8–C30 aliphatic hydrocarbons mixture under the same conditions described above and compared with available linear retention indices data in the literature.
Di Martile M., Garzoli S., Sabatino M., Valentini E., D’Aguanno S., Ragno R, & Del Bufalo D. (2021). Antitumor effect of Melaleuca alternifolia essential oil and its main component terpinen-4-ol in combination with target therapy in melanoma models. Cell Death Discovery, 7, 127.
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8

Gas Chromatography-Mass Spectrometry Analysis

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GC/MS analyses were carried out using a Perkin Elmer Clarus 500 GC equipped with a Clarus 500 mass spectrometer using the same capillary column and chromatographic conditions as was used for GC/FID analysis. The mass spectra were acquired over 40–500 amu range at 1 scan/s with ionizing electron energy of 70 eV and the ion source set at 200 °C. The transfer line was set at 280 °C, while the carrier gas was He at 1.0 mL/min.
Vitalini S., Madeo M., Tava A., Iriti M., Vallone L., Avato P., Cocuzza C.E., Simonetti P, & Argentieri M.P. (2016). Chemical Profile, Antioxidant and Antibacterial Activities of Achillea moschata Wulfen, an Endemic Species from the Alps. Molecules, 21(7), 830.
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