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2b4 pe cy5

Manufactured by BioLegend
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Sourced in United States

2B4-PE-Cy5.5 is a fluorescently-labeled antibody produced by BioLegend. It detects the 2B4 molecule, also known as CD244, which is expressed on natural killer cells, activated T cells, and other immune cell types.

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Lab products found in correlation

Cd8 pb, by BD (2 mentions) Cd3 apc h7, by BD (2 mentions) Il 2 pe, by BD (2 mentions) Cd160 apc, by BioLegend (2 mentions) Ccr7 fitc, by R&D Systems (2 mentions) Cd4 ecd, by Beckman Coulter (2 mentions) Cd8 apc h7, by BD (1 mentions) Pd1 fitc, by BD (1 mentions) Tnf α pe cy7, by BD (1 mentions) Cd28 ecd, by Beckman Coulter (1 mentions) Cd27 apc, by Beckman Coulter (1 mentions) Cd127 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd3 ecd, by Beckman Coulter (1 mentions) Cd45ra ecd, by Beckman Coulter (1 mentions) Ifn γ af700, by BD (1 mentions) Cd8 efluor625nc, by Thermo Fisher Scientific (1 mentions) Pd 1 pe cy7, by BD (1 mentions) Cd4 pb, by BD (1 mentions) Cd160 pe, by BioLegend (1 mentions)

2 protocols using 2b4 pe cy5

1

Multicolor Flow Cytometry Panel

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The following Abs were used in different combinations. CD8-PB, CD8-APCH7, CD3-APCH7, CD45RA-PECy5, PD-1-FITC, IFN-γ-APC, TNF-α-PECy7, and IL-2-PE were purchased from Becton Dickinson (BD, San Diego, CA), CD4-ECD, CD3-ECD, CD28-ECD, CD27-APC from Beckman Coulter (Fullerton, CA, USA), perforin-FITC (clone B-D48) from Diaclone (Besançon, France), CCR7-FITC from R&D Systems (Minneapolis, MN, USA), 2B4-PE-Cy5.5 and CD160-APC from Biolegend (San Diego, CA, USA) and CD127-PE-Cy7 from eBioscience (San Diego, CA, USA).
Romano E., Michielin O., Voelter V., Laurent J., Bichat H., Stravodimou A., Romero P., Speiser D.E., Triebel F., Leyvraz S, & Harari A. (2014). MART-1 peptide vaccination plus IMP321 (LAG-3Ig fusion protein) in patients receiving autologous PBMCs after lymphodepletion: results of a Phase I trial. Journal of Translational Medicine, 12, 97.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following monoclonal antibodies (mAbs) were used in different combinations. CD8-PB, CD3-APC-H7, PD-1-PECy7, PD-1-PB, IFN-γ-AF700, IL-2-PE, CD4-PB, granzyme B-AF700, perforin-APC were purchased from Becton Dickinson (BD, San Diego, CA), CD45RA-ECD, CD4-ECD from Beckman Coulter (Fullerton, CA, USA), CCR7-FITC from R&D Systems (Minneapolis, MN, USA), 2B4-PECY5.5, CD160-APC, CD160-PE from BioLegend (San Diego, CA, USA), CD4-eFluor650NC, CD8-eFluor625NC from eBioscience.
Viganò S., Banga R., Bellanger F., Pellaton C., Farina A., Comte D., Harari A, & Perreau M. (2014). CD160-Associated CD8 T-Cell Functional Impairment Is Independent of PD-1 Expression. PLoS Pathogens, 10(9), e1004380.
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