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Pe cy7 pd 1 29f 1a12

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PE/Cy7-PD-1 (29F.1A12) is a fluorescently-labeled antibody that binds to the PD-1 (Programmed Cell Death-1) receptor. It is designed for flow cytometry applications to detect and analyze PD-1 expression on cells.

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Lab products found in correlation

Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Apc cd138 281 2, by BioLegend (2 mentions) Efluor450 streptavidin, by Thermo Fisher Scientific (2 mentions) Brilliant violet 421 cd95 jo2, by BD (2 mentions) Biotinylated cxcr5 2g8, by BD (2 mentions) Recombinant mouse il 21r human fc chimera, by R&D Systems (2 mentions) Alexafluor 647 antihuman igg fcγ, by Jackson ImmunoResearch (2 mentions) Pe iga, by Southern Biotech (2 mentions) Foxp3 fix perm kit, by Thermo Fisher Scientific (2 mentions) Recombinant il 21, by Thermo Fisher Scientific (2 mentions) Antibody against il 21r 4a9, by BioXCell (2 mentions) Tgfβ receptor 1 inhibitor sb505124, by Merck Group (2 mentions) Recombinant mouse il 6, by R&D Systems (2 mentions) Collagenase 4, by Merck Group (2 mentions) Anti biotin microbead, by Miltenyi Biotec (2 mentions) Cd62l percp cy5.5 mel 14, by Thermo Fisher Scientific (2 mentions) Lag 3 pe c9b7w, by Thermo Fisher Scientific (2 mentions) Tim 3 pe 8b 2c12, by Thermo Fisher Scientific (2 mentions) Tigit apc 1g9, by BioLegend (2 mentions) C maf efluor660, by Thermo Fisher Scientific (2 mentions)

4 protocols using pe cy7 pd 1 29f 1a12

1

Multiparameter Flow Cytometry Panel

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The following antibodies were used for flow cytometry: PE-IL-17A (TC11-18H10.1), FITC-B220 (RA3-6B2), PE-α4β7 (DATK32), FITC-CD4 (RM4-5), PE/Cy7-CD38 (90), APC-CD138 (281-2), and PE/Cy7-PD-1 (29F.1A12) were purchased from Biolegend; APC-RORγt (B2D), APC-GL-7, and eFluor450-Streptavidin were from eBioscience; Brilliant Violet 421-CD95 (Jo2) and biotinylated CXCR5 (2G8) were from BD Biosciences; recombinant mouse IL-21R human FC chimera was from R&D Systems; AlexaFluor 647 antihuman IgG FCγ (polyclonal) was from Jackson ImmunoResearch; PE-IgA (polyclonal) was from Southern Biotechnology. Intracellular permeabilization was performed with Foxp3 perm/fix kit from eBioscience. Live cells were gated using Live/Dead Fixable Dead Cell stain kit from Life Technologies. Mouse recombinant IL-6, and human recombinant IL-17A, TGFβ1 were purchased from R&D Systems. Recombinant IL-21 was purchased from eBioscience. Antibody against IL-21r (4A9) was from BioXCell. Antibodies against IgA and IgD were purchased from Kirkegaard & Perry Labs and Southern Biotechnology. Anti-µ was purchased from Jackson ImmunoResearch Laboratories. All-trans-retinoic acid, TGFβ receptor I inhibitor SB505124, and collagenase IV were purchased from Sigma-Aldrich. Anti-biotin microbeads from Miltenyi were used to sort naïve IgD+ B cells.
Cao A.T., Yao S., Gong B., Nurieva R.I., Elson C.O, & Cong Y. (2015). Interleukin (IL)-21 promotes intestinal IgA response to microbiota. Mucosal immunology, 8(5), 1072-1082.
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2

Multiparameter Flow Cytometry Panel

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The following antibodies were used for flow cytometry: PE-IL-17A (TC11-18H10.1), FITC-B220 (RA3-6B2), PE-α4β7 (DATK32), FITC-CD4 (RM4-5), PE/Cy7-CD38 (90), APC-CD138 (281-2), and PE/Cy7-PD-1 (29F.1A12) were purchased from Biolegend; APC-RORγt (B2D), APC-GL-7, and eFluor450-Streptavidin were from eBioscience; Brilliant Violet 421-CD95 (Jo2) and biotinylated CXCR5 (2G8) were from BD Biosciences; recombinant mouse IL-21R human FC chimera was from R&D Systems; AlexaFluor 647 antihuman IgG FCγ (polyclonal) was from Jackson ImmunoResearch; PE-IgA (polyclonal) was from Southern Biotechnology. Intracellular permeabilization was performed with Foxp3 perm/fix kit from eBioscience. Live cells were gated using Live/Dead Fixable Dead Cell stain kit from Life Technologies. Mouse recombinant IL-6, and human recombinant IL-17A, TGFβ1 were purchased from R&D Systems. Recombinant IL-21 was purchased from eBioscience. Antibody against IL-21r (4A9) was from BioXCell. Antibodies against IgA and IgD were purchased from Kirkegaard & Perry Labs and Southern Biotechnology. Anti-µ was purchased from Jackson ImmunoResearch Laboratories. All-trans-retinoic acid, TGFβ receptor I inhibitor SB505124, and collagenase IV were purchased from Sigma-Aldrich. Anti-biotin microbeads from Miltenyi were used to sort naïve IgD+ B cells.
Cao A.T., Yao S., Gong B., Nurieva R.I., Elson C.O, & Cong Y. (2015). Interleukin (IL)-21 promotes intestinal IgA response to microbiota. Mucosal immunology, 8(5), 1072-1082.
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3

Comprehensive T Cell Phenotyping Protocol

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Splenocytes were stained with fixable viability dye eFluor780 (1:1,000) before staining for surface antigens CD4-A700 (GK1.5, 1:100), CD69-PE-Cy7 (H1.2F3, 1:300), CD62L-PerCP-Cy5.5 (MEL-14, 1:200), LAG-3-PE (C9B7W, 1:200), TIM-3-PE (8B.2C12, 1:200) (all eBioscience) and PD-1-PE-Cy7 (29F.1A12, 1:300), TIGIT-APC (1G9, 1:200), CD49b-PE (HMα2, 1:100) (all Biolegend). For staining of transcription factors and Ki67, cells were then fixed with fixation/permeabilization buffer and antibodies were diluted in permeabilization buffer (both eBioscience); Ki67-PE (SolA15, 1:200), c-Maf-eFluor660 (SYMOF1, 1:200), NFIL3-PE (S2M-E19, 1:200), FoxP3-eFluor450 (FJK-16S, 1:100). Data were collected using an LSR II flow cytometer (BD Biosciences) and analysed using FlowJo software (Treestar).
Burton B.R., Britton G.J., Fang H., Verhagen J., Smithers B., Sabatos-Peyton C.A., Carney L.J., Gough J., Strobel S, & Wraith D.C. (2014). Sequential transcriptional changes dictate safe and effective antigen-specific immunotherapy. Nature Communications, 5, 4741.
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4

Multiparameter Flow Cytometry Panel

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Splenocytes were stained with fixable viability dye eFluor780 (1:1,000) before staining for surface antigens CD4-A700 (GK1.5, 1:100), CD69-PE-Cy7 (H1.2F3, 1:300), CD62L-PerCP-Cy5.5 (MEL-14, 1:200), LAG-3- PE (C9B7W, 1:200), TIM-3-PE (8B.2C12, 1:200) (all eBioscience) and PD-1-PE-Cy7 (29F.1A12, 1:300), TIGIT-APC (1G9, 1:200), CD49b-PE (HMα2, 1:100) (all Biolegend). For staining of transcription factors and Ki67, cells were then fixed with Fixation/permeabilisation buffer and antibodies were diluted in permeabilisation buffer (both eBioscience); Ki67-PE (SolA15, 1:200), c-Maf-eFluor660 (SYMOF1, 1:200), NFIL-3-PE (S2M-E19, 1:200), FoxP3-eFluor450 (FJK-16S, 1:100). Data were collected using an LSR II flow cytometer (BD Biosciences) and analysed using FlowJo software (Treestar).
Burton B.R., Britton G.J., Fang H., Verhagen J., Smithers B., Sabatos-Peyton C.A., Carney L.J., Gough J., Strobel S, & Wraith D.C. (2014). Sequential transcriptional changes dictate safe and effective antigen-specific immunotherapy. Nature Communications, 5, 4741.
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