Azoxystrobin, boscalid and pyraclostrobin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The ternary mixed fungicide of ABP is composed of Azoxystrobin, boscalid and pyraclostrobin in an equal mass ratio (Azoxystrobin: boscalid: pyraclostrobin =1:1:1, m/m/m). Stock solutions of the ternary mixed fungicide of ABP were prepared by dissolution in acetone and were stored at 4 °C for further dilution. Methanol, acetonitrile and acetic acid (LC-MS grade) were purchased from ANPEL (Shanghai, China). The PrimeScript™ RT reagent Kit with the gDNA Eraser and TB Green® Premix Ex Taq™ II were obtained from TaKaRa (Dalian, China).
Acetic acid
Manufactured by ANPEL
Sourced in China
Acetic acid is a clear, colorless liquid with a distinctive sour odor. It is a weak organic acid with the chemical formula CH3COOH. Acetic acid is commonly used as a laboratory reagent and in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by ANPEL (4 mentions)
Acetonitrile, by ANPEL (4 mentions)
Hydrochloric acid (hcl), by Guangzhou Chemical (3 mentions)
Sodium hydroxide (naoh), by Guangzhou Chemical (3 mentions)
Methanol, by Merck Group (2 mentions)
Pyraclostrobin, by Merck Group (1 mentions)
Azoxystrobin, by Merck Group (1 mentions)
Boscalid, by Merck Group (1 mentions)
Primescript rt reagent kit with the gdna eraser, by Takara Bio (1 mentions)
Tb green premix ex taq 2, by Takara Bio (1 mentions)
N hexane, by ANPEL (1 mentions)
Menthol, by ANPEL (1 mentions)
Hplc grade acetonitrile, by ANPEL (1 mentions)
Formic acid, by ANPEL (1 mentions)
Zearalenone, by Merck Group (1 mentions)
Tectorigenin, by Yuanye Bio-Technology (1 mentions)
Matrine, by Yuanye Bio-Technology (1 mentions)
Liquiritin, by Yuanye Bio-Technology (1 mentions)
Tectoridin, by Yuanye Bio-Technology (1 mentions)
Isoliquiritigenin, by Yuanye Bio-Technology (1 mentions)
7 protocols using acetic acid
1
Ternary Fungicide Mixture Preparation
2
Pecan Organ Phytochemical Profiling
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Eight different organs of pecan, including kernel (without testa), testa, shell, shuck, leaf, branch, bark, and flower (male inflorescence), were collected in October 2018 (except the flower were in May 2019) at the scientific orchards of the Institute of Botany, Jiangsu Province and Chinese Academy of Sciences. Samples were collected from healthy adult trees of cultivar “Pawnee.” Six biological replicates were prepared for each organ, and each biological replicate contained samples from three trees. After transported back to the laboratory, the testa was peeled manually from the surface of kernels and was stored at −70°C, so as samples of other parts. Before use, all samples were powdered and homogenized in liquid nitrogen with mortars and pestles.
Menthol, acetic acid, n‐hexane, acetonitrile (HPLC grade), and formic acid were purchased from ANPEL Laboratory Technologies. Standard reference compounds, including (+)‐catechin, ellagic acid, and quercetin, were purchased from Nanjing Spring & Autumn Biological CO., Ltd.
Menthol, acetic acid, n‐hexane, acetonitrile (HPLC grade), and formic acid were purchased from ANPEL Laboratory Technologies. Standard reference compounds, including (+)‐catechin, ellagic acid, and quercetin, were purchased from Nanjing Spring & Autumn Biological CO., Ltd.
3
EPB Quantification in Surface Water
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Ethyl-paraben (EPB) was supplied by Tokyo Chemical Industry, Japan (99% pure). Hydrochloric acid (HCl) and sodium hydroxide (NaOH) were purchased from Guangzhou Chemical Reagent Factory (analytically pure). Acetic acid and acetonitrile were obtained from ANPEL Laboratory Technologies (Shanghai) Inc (HPLC-grade). Ultra-pure water (18.2 MΩ cm, Millipore Corp., Burlington, MA, USA) and surface water taken from the Pearl River were used directly without other treatment.
4
Quantitative Analysis of Mycotoxins
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Ochratoxin A standard was purchased from Romer Lab (Getzersdorf, Austria). Zearalenone was bought from Sigma (Shanghai, China) and made 1 mg/mL stock solution in methanol solvent in this current research investigation. Then this stock solution was then diluted with HPLC grade methanol to achieve the suitable work solutions. Before HPLC analysis of the sample and standard, OTA and ZEA solutions were preserved and refrigerated in darkness at −20 °C. Acetonitrile, methanol, water, ethyl acetate (everything were HPLC purity) and acetic acid were purchased from ANPEL laboratory Technologies, Shanghai, China.
5
Characterization of Botanical Compounds
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Chlorogenic acid, neoChlorogenic acid, cryptoChlorogenic acid, arctiin, arctigenin, tectoridin, tectorigenin, iridin, irisflorentin, irigenin, matrine, oxymatrine, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, and galuteolin were obtained from the Shanghai YuanYe Biotechnology Co., Ltd. (Shanghai, China) for use as references. Glycyrrhizic acid and glycyrrhetinic acid were purchased from China’s National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). The purity of the reference compounds was >98% based on high-performance liquid chromatography (HPLC) analysis. SJLYKL was prepared by the Shanghai Liantang pharmacy (Shanghai, China). Deionized water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). Ammonium acetate (HPLC grade; purity ≥98.0%) and acetic acid (HPLC grade; purity ≥99.7%) were provided by ANPEL Laboratory Technologies (Shanghai, China). Chromatographic grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany).
6
Extraction of Jujube Flavonoids using DESs
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Methanol, acetonitrile, and acetic acid were purchased from ANPEL. All solvents were of HPLC gradient grade. Ultra-pure water in-house was prepared using the Milli-Q water purification system (Millipore, Bedford, MA, USA). Choline chloride was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Malic acid, acetic acid, citric acid, fructose, glucose, urea, ethylene glycol, sorbitol, xylitol, and glycerol were obtained from YongSheng Fine Chemical Co. Ltd. (Tianjin, China). Jujube (Fig. 1 a) was purchased from the local market (Shihezi, China). Jujube fruits were refrigerated at -18 °C for 24 h and thereafter crushed to powder. Samples were refrigerated at -18 °C to prevent moisture absorption and agglomeration.![]()
Xinjiang jujube were used as the experimental materials (a). Extraction yields of different DESs and conventional solvents for flavonoids (b).
7
Vitamin E HPLC Quantification Protocol
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Vitamin E standards for high-performance liquid chromatography (HPLC) were purchased from Sigma Aldrich (St. Louis, MO, USA). Methanol, hexane, isopropyl alcohol, acetic acid, and ammonium acetate of HPLC grade were purchased from ANPEL Scientific instrument Co., Ltd. (Shanghai, China). Other chemicals and reagents used were of analytical grade.
The pot experiment was conducted in an artificial climate chamber (RDN-1000B-4, Ningbo southeast Instrument Co., Ltd., Ningbo, China) in the Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China. The conditions inside the climate chamber were set at a temperature of 30 °C, in a dark environment, with a relative air humidity of 80%.
The pot experiment was conducted in an artificial climate chamber (RDN-1000B-4, Ningbo southeast Instrument Co., Ltd., Ningbo, China) in the Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China. The conditions inside the climate chamber were set at a temperature of 30 °C, in a dark environment, with a relative air humidity of 80%.
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