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Mouse rat estradiol kit

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The Mouse/Rat Estradiol Kit is a laboratory assay used to quantitatively measure the concentration of estradiol, a steroid hormone, in mouse or rat biological samples. The kit provides the necessary reagents and materials to perform the analysis.

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Mouse/Rat Estradiol ELISA kit (21 citations) Estradiol (10 citations)

2 protocols using mouse rat estradiol kit

1

Determining Estrous Cycle in Female Rats

Cited 1 time
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The estrous cycle phase of female rats was determined for 8 consecutive days at the beginning of the housing period and during 10 consecutive days prior to behavioral testing using vaginal smear cytology [28 –30 (link)]. Length of estrous cycle and cyclicity was determined by the frequency of proestrus presentations within the time measured. We also measured levels of estrogen from blood samples collected before the perfusion procedure to verify the phases of the estrous cycle. Plasma serum levels for estradiol were determined by enzyme-linked immunosorbent assay (ELISA) using a mouse/rat estradiol kit (Calbiotech, cat#ES180s), using an antibody raised in rabbit against estradiol. The sensitivity of this kit is 3 pg/ml; previously used to measure plasma levels of estradiol in Sprague-Dawley rats [31 ].
Ramos-Ortolaza D.L., Doreste-Mendez R.J., Alvarado-Torres J.K, & Torres-Reveron A. (2017). Ovarian hormones modify anxiety behavior and glucocorticoid receptors after chronic social isolation stress. Behavioural brain research, 328, 115-122.
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2

Corticosterone Quantification by ELISA

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Plasma serum levels for corticosterone were determined by enzyme-linked immunosorbent assay (ELISA) using a mouse/rat estradiol kit (Calbiotech, cat#ES180s), using an antibody raised in rabbit against corticosterone following the kit protocol instructions.
Ramos-Ortolaza D.L., Doreste-Mendez R.J., Alvarado-Torres J.K, & Torres-Reveron A. (2017). Ovarian hormones modify anxiety behavior and glucocorticoid receptors after chronic social isolation stress. Behavioural brain research, 328, 115-122.
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