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6 protocols using sodium dodecyl sulfate (sds)

1

Synthesis of Inorganic Compounds

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Magnesium nitrate hexahydrate (Mg(NO3)2 6H2O) was obtained from Sigma-Aldrich. Gallium nitrate hydrate (Ga(NO3)3 xH2O, 99.9%) was purchased from Alfa Aesar. Potassium iodide (KI) and sodium carbonate (Na2CO3) were supplied by Merck Millipore. Potassium hydroxide (KOH) was obtained from Carlo Erba Reagents. Brucite (Mg(OH)2) was obtained from Nacalai Tesque. Sodium dodecyl sulfate (C12H25NaO4S, >95.0%), hexanol (C6H14O, >98.0%), and benzene (C6H6, >99.5%) were supplied by Tokyo Chemical Industry Co., Ltd. These chemicals were used without further purification. Deionized water was obtained from a Milli-Q system (18.2 MΩ-cm, Merck Millipore, Darmstadt, Germany).
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2

Antioxidant Evaluation of Compounds

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EyPC, LID, DIB, Trolox (TRO), 1,1-diphenyl-2-picrylhydrazyl
free radical (DPPH), hydrogen peroxide (H2O2), sodium dodecyl sulfate, 2-thiobarbituric acid (TBA), and 2,6-di-tert-butyl-p-cresol (BHT) were purchased from the Tokyo
Chemical Industry. 1,1,3,3-Tetraethoxypropane, chloroform, and diethyl
ether were purchased from FUJI-FILM Wako Pure Chemical Corporation
(Osaka, Japan). Ethanol (95%) was purchased from Kanto Chemical Co.,
Inc.(Tokyo, Japan). All other reagents used were of the highest commercially
available grade.
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3

Microemulsion Electrolyte for Supercapacitors

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The microemulsion electrolyte consisted of a mixture of 60 wt.% distilled water, 8.33 wt.% sodium dodecyl sulfate (SDS) (Tokyo Chemical Industry, Tokyo, Japan), 16.67 wt.% n-butanol (Sigma-Aldrich), and 15 wt.% cyclohexane (Sigma-Aldrich). A clear homogeneous solution was observed which confirmed the formation of the microemulsion. Then 0.1 M NaCl (Sigma-Aldrich) was added to the mixture. This electrolyte was used for all supercapacitor experiments.
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4

Nanoparticle-Based Drug Delivery System

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DOX was supplied by Boryung Pharmaceutical Co., Ltd. (Seoul, Korea). PLGA (Mn: 45‒55 kDa; LA:GA = 50:50) was obtained from PolySciTech (Akina, Inc., West Lafayette, IN, USA). ICG, PHL, and sodium dodecyl sulfate (SDS) were acquired from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Dil, poly(vinyl alcohol) (PVA; 30–70 kDa molecular weight (MW)), and SA (N-acetylneuraminic acid) were supplied by Sigma–Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI 1640 (developed by Roswell Park Memorial Institute), penicillin–streptomycin, and FBS were provided by Life Technologies, Corp. (Carlsbad, CA, USA). All other reagents were analytical grade.
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5

Graphene Nanofluids Preparation and Characterization

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In this study, DW (distilled water) was used for the base fluids that were produced through the membrane-type DW maker, which maintained a water quality under 10 ppm for the total dissolved solids (TDS). Graphene with 7-nm thickness and a 40-nm size with a specific surface area of 100 m2/g and purity of 99.9% was purchased from Graphene supermarket, and surfactant SDS and SDBS were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan) (SDS), TOKYO Chemical Industry Co., Ltd. (Tokyo, Japan) (SDBS) was used. First, 0.1 g of graphene was pulverized with a ball size of 1 mm, a ball-milling speed of 200 rpm, and a ball-milling time of 60 min using (a) the planetary ball mill instrument. After that, the ratio of graphene (0.1 wt %) dissolved in the water and the surfactant SDS and SDBS were prepared for adding in ratios of 1:3, 1:2, 1:1, 2:1, and 3:1. Furthermore, ultrasound excitation was carried out for 40 min to make the graphene nanofluids, and the degrees of nanoparticle dispersion were measured by using UV spectroscopy.
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6

HPLC-ECD Quantification of DA and 5-HT

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Dialysis samples were analyzed for DA and 5-HT using high-performance liquid chromatography coupled with electrochemical detection (HPLC-ECD) (model HTEC-500, from EiCOM Co., Kyoto, Japan). Aliquots of dialysate (5 μL) were injected onto an analytical column (particle size 2 mm, PP-ODS 4.6 × 30 mm maintained at 25 °C) and eluted using an isocratic mobile phase consisting of a 100 mM NaH2PO4 buffer containing 134 μM 2Na-EDTA, 3.27 mM SDS and 0.75 % (v/v) Methanol (pH 6.0) delivered at 400 μL/min. Both monoamine molecules were detected electrochemically using a graphite working electrode set at +450 mV against a silver-silver chloride reference electrode. Quantification was performed using external calibration curves that were constructed daily using freshly prepared standards. All chemicals for the mobile phase and chromatographic standards were of the highest obtainable grade from Sigma-Aldrich Co. (St. Louis, MO, USA) except for SDS, which was purchased from TCI-ACE, Tokyo Chemical Industry Co. (Tokyo, Japan).
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