Cell invasion was assayed using the CytoSelect 24-well Cell Migration and Invasion Assay according to the manufacturer’s instruction (Cell Biolabs, San Diego, CA). In brief, MCF-7 cells were resuspended in serum-free DMEM containing 0.1% bovine serum albumin. The cell suspension (1 × 106 cells/ml) was added to the top insert, whereas DMEM containing 10% FBS was added to the bottom chamber. The cells were incubated at 37 °C for 24 h, and the insert was transferred to a well containing Cell Stain Solution. After incubation for 10 min, the stained insert was washed and air-dried. The migratory cells were counted with a light microscope.
Cytoselect 24 well cell migration and invasion assay
Manufactured by Cell Biolabs
Sourced in United States
The CytoSelect 24-well Cell Migration and Invasion Assay is a laboratory product that measures the ability of cells to migrate through a membrane or invade a matrix. It provides a quantitative assessment of these cellular processes.
Automatically generated - may contain errors
9 protocols using cytoselect 24 well cell migration and invasion assay
1
Cell Invasion Assay Using CytoSelect
2
Inhibition of NKX2-1-AS1 Impacts Wound Healing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We tested the effect of reduced NKX2-1-AS1 levels on the wound healing behavior of H441 cell monolayers upon induced injury. Healing of a scratch in confluent H441 cells transfected with non-silencing control or NKX2-1-AS1 siRNAs was analyzed using the analytical function in Adobe Photoshop CS4 Suite. Migration and invasion were determined using CytoSelect™ 24-well Cell Migration and Invasion Assay (8 µm, Fluorometric Format) (Cell Biolabs, Inc.). Cell migration analysis was performed 48 h and 72 h after NKX2-1-AS1 siRNAs transfection using polycarbonate membrane inserts. After 24 h migrating cells were dissociated from the membrane and detected in a fluorescence reader using the CyQuant® GR Dye (Invitrogen). For invasion assays we used basement membrane-coated inserts following the same protocol.
3
Cell Migration and Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The assays were carried out using Cytoselect 24-well cell migration and invasion assay according to manufacture instructions (Cell Biolabs, Inc. CBA-100-C, San Diego, CA, USA). Cells were seeded at 5 × 105 cells per well in a serum-free medium supplemented with 0.1% BSA. The U87 cells were induced to migrate or invade toward medium containing 10% FBS alone or with 1% CD-NHF (10 µg/mL), K252A (5 nM), AKTVIII (1.7 µM), CD-NHF-K252A or CD-NHF-AKTVIII for 24 h in the CO2 incubator. Treated groups contained, in the inserted medium, the same treatment as in wells. Twenty-four hours post-seeding, non-invading cells were removed with a cotton swab. The remaining cells were fixed, stained with DAPI, and analyzed by fluorescence microscopy (10× magnification; Zeiss Axio Observer Z.1 microscope, TissueGnostics rig, Vienna, Austria). Sixteen images were acquired per well using TissueFAXS 4.2 software (Vienna, Austria) and quantified by using ImageJ software.
4
Cell Migration and Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell migration and invasion were assessed using a Cytoselect 24-Well Cell Migration and Invasion Assay (CELL BIOLABS). For migration and invasion measurements, transfected Huh7 and Hep3B cells were seeded at 5.0 × 105 cells/well. The cells that invaded or migrated to the underside of the chamber were harvested and measured according to the manufacturer’s protocol. Results were determined from triplicate wells in three independent experiments and expressed as a percentage relative to control.
5
Cell Growth, Migration, and Invasion Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cell growth assay, cells were seeded on 96-well plates in hexaplicate at 500 cells per well. Cells were allowed to grow for 24 h, and were then cultured for 1, 4, 7, and 10 days. Cell growth assays were performed using Cell Counting Kit-8 (Dojindo) according to the manufacturer’s instructions. OD450 value was measured, and the ratio to the value at day1 was plotted as the means ± SD of three experiments. The statistical differences were analyzed using Student’s t test. For cell migration and invasion assays, CytoSelect™ 24-Well Cell Migration and Invasion Assay (Cell Biolabs, Inc., CBA-100-C, CA, USA) was used according to the manufacture’s instruction. Briefly, cell suspensions containing 1.0 × 106 cells/ml in DMEM were prepared, and 300 μl of each suspension was added to insert chambers that had been placed in 24 well plates. Three-hundred (300) μl of DMEM containing 10% FBS was added to the well with the insert chamber. After 24 h culture, cells were stained with Cell Stain Solution, washed with water, and lysed with Extraction Solution. Cell migration and invasion were quantified at OD 560 nm in a plate reader. The ratio of sh-γ1 to Ctrl was plotted as mean ± SD of three experiments. The statistical difference was analyzed using Student’s t test.
6
Cell Invasion Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell invasion was measured by CytoSelect™ 24‐Well Cell Migration and Invasion Assay (Cell Biolabs, San Diego, CA) according to the manufacturer's instructions. Briefly, the basement membrane layer of the cell culture inserts was rehydrated with serum‐free media for 1 h. Media (10% FBS, 500 μL) was added to the lower well of the migration plate. Cells were prepared and suspended in serum‐free media and were added to the inside of each insert and incubated for 48 h in an incubator. The media was aspirated from the inside of the insert, and nonmigratory cells were then removed. The insert was transferred to a clean well containing 400 μL of cell stain solution and incubated for 10 min and washed. Each insert was then transferred to an empty well and extraction solution was added at 200 μL per well, and then incubated for 10 min in an orbital shaker. From each sample, 100 μL was transferred to a 96‐well microtiter plate and measured for OD560 nm (BioTek, Winooski, VT).
7
Cell Migration and Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell migration and invasion were measured using the CytoSelectTM 24-Well Cell Migration and Invasion Assay (Cell Biolabs, San Diego, CA). Stained invasive and migratory cells were observed with a Nikon microscope and quantified using a SpectraMax plate reader at 560 nm.
8
Cell Migration and Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Additionally, pretreated 3D spheroids and organoid-derived cells were tested for their ability to migrate and invade for 24–48 h using the CytoSelectTM 24-Well Cell Migration and Invasion Assay according to the manufacturer’s instructions (8 µm, Colorimetric Format), Cat# CBA-100C, Cell Biolabs, San Diego, CA, USA). After microscopic images for migration and invasion were obtained with an inverted microscope (Leica Microsystems), labeled cells from membranes were extracted and tested for optical density (OD) at 560 nm at a plate reader.
9
Quantitative Transwell Assay of Crocin-Mediated Cell Migration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CytoSelect TM 24-well cell migration and invasion assay (Cell Biolabs, Inc.) was utilized for the cell transwell migration assay. The assay used an 8-µm pore size and a colorimetric format. It was applied to assess the effect of crocin on the migration and invasion of MDA-MB-231 cells. The assay was performed according to the manufacturer’s protocol.
In brief, the cells were treated with 60, 120, and 240 µM of crocin for 48 hours. After the treatment period, a cell suspension of 500 000 cells/ml was prepared and transferred into the upper chamber of the plate. The bottom chamber was loaded with 150 µl of complete RPMI1640 medium containing 10% FBS as a chemoattractant. The plate was then placed in a humidified incubator for 24 h.
In response to the chemoattractant, migratory cells passed through the polycarbonate membrane pores and invaded the bottom of the membrane. Finally, the migrated cells were stained, extracted, and quantified at an absorbance of 560 nm as outlined in the manufacturer’s protocol.
In brief, the cells were treated with 60, 120, and 240 µM of crocin for 48 hours. After the treatment period, a cell suspension of 500 000 cells/ml was prepared and transferred into the upper chamber of the plate. The bottom chamber was loaded with 150 µl of complete RPMI1640 medium containing 10% FBS as a chemoattractant. The plate was then placed in a humidified incubator for 24 h.
In response to the chemoattractant, migratory cells passed through the polycarbonate membrane pores and invaded the bottom of the membrane. Finally, the migrated cells were stained, extracted, and quantified at an absorbance of 560 nm as outlined in the manufacturer’s protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!