The CO2 used for the extraction was 99.97% pure (Messer, Tehnogas AD, Rakovica, Serbia). Nicotine standard (99% purity) was purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). All other solvents used were of analytical reagent grade.
Nicotine standard
Manufactured by Merck Group
Sourced in Germany
The Nicotine standard is a laboratory reference material used for the calibration and verification of analytical instruments that measure the concentration of nicotine in various samples. It provides a known and consistent concentration of nicotine to ensure accuracy and precision in analytical results.
Automatically generated - may contain errors
Lab products found in correlation
Sodium acetate, by RCI Labscan (1 mentions)
Trimethylamine, by Loba Chemie (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Methanol, by RCI Labscan (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Ethanol, by RCI Labscan (1 mentions)
Chloroform, by RCI Labscan (1 mentions)
Glacial acetic acid, by RCI Labscan (1 mentions)
N heptadecane, by Merck Group (1 mentions)
Suprasolv, by Merck Group (1 mentions)
3 protocols using nicotine standard
1
Nicotine Extraction Using CO2
2
Nicotiana tabaccum Leaf Extract Analysis
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The light air-cured leaves of N. tabaccum were received from Maejo Tobacco Experiment Station, Chiang Mai, Thailand, under the TOAT organization. The plant voucher specimen (No. 0023267) was deposited at the Herbarium of the Faculty of Pharmacy, Chiang Mai University (CMU), and authenticated by the CMU staff botanist. Chloroform, ethanol, glacial acetic acid, methanol, and sodium acetate were purchased from RCI Labscan Limited (Bangkok, Thailand). Sodium carbonate and sodium hydroxide were purchased from Merck (Darmstadt, Germany). ethanol (99% v/v) was purchased from Liquor Distillery Organization (Bangkok, Thailand). Trimethylamine was purchased from Loba Chemie Pvt, Ltd. (Mumbai, India). Nicotine standard was purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydroxypropyl methylcellulose E15 (HPMC E15, AnyCoat®-C AN15) was purchased from Lotte Fine Chemical Co., Ltd. (Seoul, Korea).
3
Nicotine Determination Protocol
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For nicotine determination we used dichloromethane (SupraSolv, Merck, Darmstadt, Germany), 99 % n-heptadecane (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany), and ≥99 % purity nicotine standard (Sigma-Aldrich).
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