Hrp labeled polymer conjugated secondary antibodies against rabbit igg

Immunohistochemistry staining of formalin-fixed and paraffin-embedded tissues was performed as previously described^{26 (link)}. After micro-sectioning at 4 μm thickness, the sections underwent heat-induced antigen retrieval for 3 min in EDTA buffer (pH 9.0; Dako, Carpinteria, CA). Sectioned slides were then incubated overnight with anti-human NIS (1:100 dilution; Santa Cruz Biotechnology, #sc-134515) at 4 °C. This was followed by incubation with HRP-labeled polymer-conjugated secondary antibodies against rabbit IgG (Dako) for 30 min at room temperature. A color reaction was induced using a ready-to-use 3,3′-diaminobenzidine substrate-chromogen solution (Dako) for 5 min, followed by washing with distilled water. Finally, sections were lightly counterstained with Mayer’s hematoxylin for 30 s before dehydration and mounting.

After micro-sectioning formalin-fixed and paraffin-embedded tissues at 4 μm thickness, heat-induced antigen retrieval was performed for 3 min in pH 9.0 EDTA buffer (Dako). Sectioned slides were then incubated overnight with an anti–human NIS rabbit polyclonal antibody (1:100 dilution; Santa Cruz Biotechnology) at 4 °C. This was followed by incubation with HRP-labeled polymer-conjugated secondary antibodies against rabbit IgG (Dako, CA) for 30 min at room temperature. A color reaction was induced using the ready-to-use 3,3′-diaminobenzidine substrate chromogen solution (Dako) for 5 min, followed by washing with distilled water. Sections were lightly counterstained with hematoxylin for 30 seconds before dehydration and mounting.