Ab246529

Manufactured by Abcam

Sourced in United Kingdom, United States

Ab246529 is a laboratory equipment product. It is designed for use in scientific research and experiments. The core function of this product is to facilitate specific tasks or processes within a laboratory setting. No further details are provided to maintain an unbiased and factual approach.

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Lab products found in correlation

Ab187396, by Abcam (2 mentions) Sea056ra, by Cloud-Clone (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Ab102526, by Abcam (1 mentions) Ab65608, by Abcam (1 mentions) Ab100768, by Abcam (1 mentions) Ab100772, by Abcam (1 mentions) Ab176648, by Abcam (1 mentions) Ab46070, by Abcam (1 mentions) Ab39401, by Abcam (1 mentions) Spectramax i3x, by Molecular Devices (1 mentions) Ab234579, by Abcam (1 mentions) Ab263883, by Abcam (1 mentions)

7 protocols using ab246529

Comprehensive Cardiac Biomarker Analysis

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Samples from the obtained heart tissues were homogenized in 10% phosphate buffer to measure numerous biochemical parameters. ELISA kits were consumed to measure cardiac enzymes; creatine phosphokinase (CPK; ab187396), creatine phosphokinase heart (CK-MB; ab187396), cardiac troponin T (cTnT; ab246529), and cardiac troponin I (cTnI; ab246529); apoptotic markers; caspase 3 (ab39401), Caspase 9 (ab65608), and inflammatory mediators; tumor necrosis factor-alpha (TNF-α; ab46070), interleukin 6 (IL-6; ab100772), interleukin 1 beta (IL-1β; ab100768), and nuclear factor-κ-B (NFκB; ab176648) (all obtained from Abcam, Eugene, OR, USA), following the manufacturer’s protocols and using a microplate reader (SpectraMax i3x, Molecular Devices, USA).

Mohamed M.E., Abduldaium M.S, & Younis N.S. (2021). Cardioprotective Effect of Linalool against Isoproterenol-Induced Myocardial Infarction. Life, 11(2), 120.

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Myocardial Injury Biomarker Quantification

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Levels of creatine kinase-MB (CK-MB) (354798; USBiological, MA, Swampscott, USA), creatine kinase (CK) (ab187396; Abcam, Cambridge, UK), cardiac troponin I (cTnI) (ab246529; Abcam), and myoglobin (MB) (ab260068; Abcam) in myocardial tissues and cardiomyocytes with H/R were measured using corresponding enzyme-linked immunosorbent assay kits by following the protocols of the manufacturers.

Sun C., Zhang X., Yu F., Liu C., Hu F., Liu L., Chen J, & Wang J. (2021). Atractylenolide I alleviates ischemia/reperfusion injury by preserving mitochondrial function and inhibiting caspase-3 activity. The Journal of International Medical Research, 49(2), 0300060521993315.

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Serum Protein Quantification in Rats

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Troponin I (ab246529, Abcam, Cambridge, UK), iNOS (RPA837Ra02, Cloud-Clone Corp., Wuhan, China) and IL-10 (SEA056Ra, Cloud-Clone Corp., Wuhan, China) ELISA kits were used to measure protein expression in rat serum in accordance with the manufacturer’s instructions. The standard preparations were added to the first and second wells, followed by serial dilutions. Sample diluent and samples were added to each well at 37 °C for 30 min. After washing, secondary antibodies were added to each well at 37 °C for 30 min. After washing, chromogenic agents A and B were added to each well at 37 °C in the dark for 15 min. Termination solution was then added to each well. A microplate reader (Bio-Rad, USA) was used for detection. Sample concentrations were calculated according to the standard curve.

Xie D., Li M., Yu K., Lu H, & Chen Y. (2020). Etomidate alleviates cardiac dysfunction, fibrosis and oxidative stress in rats with myocardial ischemic reperfusion injury. Annals of Translational Medicine, 8(18), 1181.

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Cardiac Biomarkers for Injury Assessment

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Cardiac troponin (cTnT) and lactate dehydrogenase (LDH-1) are specific biomarkers of cardiac injury and widely employed in clinical and animal studies to assess the extent of cardiac injury22 (link)
^,23 (link). The release of these markers in the plasma is an indication of cardiac injury, and their levels are in proportion to extent of cardiac injury. The levels of cTnT in the plasma were measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit (ab246529; Abcam Inc, Toronto, Canada), and the levels of LDH-1 in the plasma were measured by a colorimetric method using a commercially available kit (ab102526; Abcam Inc, Toronto, Canada).

Zhuo X, & Jiang H. (2022). Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway. Acta Cirúrgica Brasileira, 37(2), e370208.

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Serum Biomarkers Quantification Protocol

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Serum samples were separated after centrifugation of blood samples at 3,000 rpm for 20 min. Serum troponin I (ab246529, Abcam, Cambridge, UK) and serum interleukin-6 (R6000B, R&D Systems, Minneapolis, MN, USA) levels were measured by ELISA kits.

Amioka N., Takaya Y., Nakamura K., Kondo M., Akazawa K., Ohno Y., Ichikawa K., Nakayama R., Saito Y., Akagi S., Miyoshi T., Yoshida M., Morita H, & Ito H. (2022). Impact of shear wave dispersion slope analysis for assessing the severity of myocarditis. Scientific Reports, 12, 8776.

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Biomarker Evaluation in Acute Myocardial Infarction

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Serum of growth stimulation expressed gene 2 (ST2) (ab255716, Abcam, England), NT-proBNP (NBP2-68140, Novus Biologicals, United States), and cTnI (ab246529, Abcam, England) were determined according to the manufacturers’ protocols for the respective kits. The level of cTnI in serum was measured at 24 h after AMI. The level of ST2 and NT-proBNP in serum was measured at 28 d after AMI.

Tao W., Yang X., Zhang Q., Bi S, & Yao Z. (2023). Optimal treatment for post-MI heart failure in rats: dapagliflozin first, adding sacubitril-valsartan 2 weeks later. Frontiers in Cardiovascular Medicine, 10, 1181473.

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Quantifying Cardiac Biomarkers

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Corresponding enzyme-linked immunosorbent assay kits (ab246529, ab285275, ab263883, and ab234579; Abcam) were employed to measure the contents of Troponin I, creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). The aforementioned biochemical indexes were measured strictly per the specific operation steps and cautions in the protocols of the corresponding kits. The optical density was analyzed and calculated with a microplate reader (Beckman Coulter, CX7, Brea, CA, USA) and the concentrations of the samples were calculated based on the corresponding formula or standard curve and regression equation of biomarkers.

Xiong J., Ding B., Zhu W., Xu L, & Yu S. (2023). Exosomes from Adipose-Derived Mesenchymal Stem Cells Protect Against Cyclophosphamide-Induced Cardiotoxicity in Rats. International heart journal, 64(5).

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