Rabbits were anesthetized with an intramuscular injection of xylazine 5 mg/kg (Virbaxil® 2%; Virbac Laboratories, Carros, France) and tiletamine-zolazepam 15 mg/kg (Zoletil® 100, Virbac Laboratories, Carros, France), euthanized with an intravenous injection of embutramide-mebenzonio-tetracaine 26 mg/kg (Tanax®, Intervet Italia Srl, Peschiera Borromeo, Italy), then, after death, jugulated.
Virbaxil 2
Manufactured by Virbac
Sourced in France
Virbaxil® 2% is a laboratory equipment product manufactured by Virbac. It is a concentrated solution intended for use as a disinfectant.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using virbaxil 2
1
Rabbit Euthanasia and Jugulation Protocol
2
Euthanasia Protocol for Rabbit Research
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The study required the subjects to be euthanized at different stages and submitted to postmortem examination and organic-tissue sample collection. The rabbits were first anesthetized with an intramuscular injection of xylazine 5 mg/kg (Virbaxil® 2%; Virbac Laboratories, Carros, France) and tiletiletamine-zolazepam 15 mg/kg (Zoletil® 100, Virbac Laboratories, Carros, France) and euthanized with an intravenous injection of embutramide-mebenzonio-tetracaine 26 mg/kg (Tanax®, Intervet Italia Srl, Peschiera Borromeo, Italy) followed by exsanguination.
3
Rabbit Spine Arthrodesis Protocol
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Surgery was performed in 32 rabbits at the age of 5–6 weeks under general anesthesia (GA) and anatomy of O. cuniculus was consulted in advance.19 GA was administered by an intramuscular injection of xylazine 5 mg/kg (Virbaxil® 2%, Virbac Laboratories, Carros, France) and tiletiletamine-zolazepam 15 mg/kg (Zoletil® 100, Virbac Laboratories, Carros, France). Subsequent skin anesthesia was obtained with a subcutaneous injection of 2% lidocaine hydrochloride (1 ml/animal).
Rabbits were operated according to a modified “Wisconsin” technique or extra-canal dorsal T1–T12 vertebral arthrodesis, corresponding to posterior vertebral arthrodesis in bipeds, as punctually indicated in previous our works.13 (link)14 (link)15 (link) Multiple fragments of demineralized bone matrix (Grafton DBM, Osteotech Inc., Eatontown, NJ, USA) were applied, during the surgery, on each side of the spinous processes of the thoracic spine to favor fusion.
The operation lasted approximately 50 min on average for each rabbit. Surgery was considered successful and at recovery from GA, the operated rabbits did not show any motor disorder.
Rabbits were operated according to a modified “Wisconsin” technique or extra-canal dorsal T1–T12 vertebral arthrodesis, corresponding to posterior vertebral arthrodesis in bipeds, as punctually indicated in previous our works.13 (link)14 (link)15 (link) Multiple fragments of demineralized bone matrix (Grafton DBM, Osteotech Inc., Eatontown, NJ, USA) were applied, during the surgery, on each side of the spinous processes of the thoracic spine to favor fusion.
The operation lasted approximately 50 min on average for each rabbit. Surgery was considered successful and at recovery from GA, the operated rabbits did not show any motor disorder.
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