Balb c

BALB/c and C57BL/6 mice (female, 6-weeks-old) were purchased from Orient Bio and maintained under pathogen-free conditions. The animal study was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of Sungkyunkwan University School of Medicine, which is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC International) and abides by the Institute of Laboratory Animal Resources (ILAR) guide. Murine 4T1 (triple-negative breast cancer), murine TC1 (cervical cancer), and human THP-1 cells (American Type Culture Collection, ATCC) were cultured in RPMI medium (Thermo Fisher Scientific). Human C33a cervical cancer cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium (Thermo Fisher Scientific). The cell lines used in this study are not listed in the database of misidentified cell lines. The cell lines used in our study were tested to be free of mycoplasma contamination.

Nude mice (Balb/c, 5-week-old males) were obtained from Orientbio, Inc., (Seoul, South Korea) and maintained in a laminar air-flow cabinet under specific pathogen-free conditions. A549 cells were transduced with control shRNA virus (shCTR) or BRD7 shRNA virus (shBRD7) and a 0.1 ml suspension of each containing 1 × 10⁶ cells was subcutaneously injected into the right flank of each mouse. When the tumor size reached 150 mm³ for both the shCTR and shBRD7 groups, the mice treated with each shRNA were randomly divided into two groups (10 mice/group). The experimental group of mice received an intratumoral injection of vehicle or interferon-γ (IFN-γ, 1 × 10⁵ international units [IU]) solution three times a week. Two perpendicular diameters of the tumors were measured three times per week with a caliper square by the same investigator, and the tumor volume was calculated using the following equation: Tumor volume (V) mm³ = (smaller diameter)² x (larger diameter) x (π/6). The experiment was terminated 5 weeks after tumor cell implantation because the tumors in the control mice began to show signs of possible necrosis. The use of these animals and the experimental procedures were approved by the Institutional Animal Care and Use Committee of the Korea Institute of Radiological and Medical Sciences.

Seven-week-old male C57BL/6, BALB/c, and ICR mice were purchased from Orient Bio, Inc. (Seongnam, Korea). The mice were housed in a temperature-controlled environment (22 ± 3 °C) with a relative humidity of 50 ± 20%, a 12 h light/dark cycle, and ventilated with air (10–20 times/h). The mice were provided with pellets that were specifically produced for experimental animals (PMI Nutrition International, Richmond, IN, USA) and UV-irradiated (Steritron SX-1; Daeyoung, Seoul, Korea) and filtered (1 μm-pore filter) tap water. All experimental procedures were approved by the Institutional Animal Care and Use Committee of the Korea Institute of Toxicology (IACUC #2108-0023). The mice in the PS, PP, and PVC groups received intratracheal instillation of 5 mg/kg PS, PP, or PVC in 50 μL of saline solution for two weeks using an automatic video instillator. Mice in the VC group were instilled with saline using the same method. The mice were sacrificed on day 15.

BALB/c and C57BL/6 mice were purchased from OrientBio (Sungnam, Korea). Mice were housed at 21Dis°C with 51–54% humidity in a pathogen-free environment under a 12-h light/dark cycle and were given full access to food and water. The Animal Care and Use Committee of Ewha Womans University College of Medicine approved all experimental procedures and protocols (Seoul, Korea; approval no. EUM20-014).