Cell suspensions were incubated with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies). Fc receptors were blocked using FcR block (Miltenyi) followed by staining with antibodies against surface molecules CD1c (AD5‐8E7; Miltenyi), CD3 (SP34‐2 and SK7; BD), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3G8; Biolegend), CD19 (HIB19; Biolegend), CD20 (L27; BD), CD45 (HI30; Biolegend), CD56 (HCD56; Biolegend), CD66abce (TET2; Miltenyi), CD80 (2D10; Biolegend), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi), CCR7 (G043H7; Biolegend), and HLA‐DR (G46‐6; BD) for 15 min at 4°C in PBS with 2% FCS and fixed with 1% paraformaldehyde. Cells were analyzed using an LSRII flow cytometer (BD) and data were analyzed using FlowJo X software (Tree Star).
Cd3 sp34 2 and sk7
Manufactured by BD
The CD3 (SP34-2 and SK7) is a laboratory equipment product. It is used to detect and enumerate CD3-positive cells, which are a type of T lymphocyte. The product utilizes flow cytometry technology to provide quantitative measurements of CD3-positive cells in a biological sample.
Automatically generated - may contain errors
Lab products found in correlation
Cd66abce tet2, by Miltenyi Biotec (2 mentions)
Cd14 m5e2, by BD (2 mentions)
Cd1c ad5 8e7, by Miltenyi Biotec (2 mentions)
Cd11c b ly6, by BD (2 mentions)
Cd19 hib19, by BioLegend (2 mentions)
Cd20 l27, by BD (2 mentions)
Cd123 7g3, by BD (2 mentions)
Cd141 ad5 14h12, by Miltenyi Biotec (2 mentions)
Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Cd80 2d10, by BioLegend (1 mentions)
Cd16 3g8, by BioLegend (1 mentions)
Cd45 hi30, by BioLegend (1 mentions)
Cd56 hcd56, by BioLegend (1 mentions)
Ccr7 g043h7, by BioLegend (1 mentions)
Hla dr g46 6, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Fcr block, by Miltenyi Biotec (1 mentions)
Cd45ra hi100, by BioLegend (1 mentions)
Cd16 3ge, by BioLegend (1 mentions)
Cd56 hcd56, by BD (1 mentions)
2 protocols using cd3 sp34 2 and sk7
1
Multiparameter Flow Cytometry Panel
2
Comprehensive PBMC Phenotypic Analysis
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Phenotypic analysis was performed on PBMCs using panels containing Live/Dead Blue (Life technologies), CD1c (AD5-8E7; Miltenyi), CD3 (SP34-2 and SK7; BD), CD10 (HI10a; Biolegend), CD11c (B-Ly6, BD), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD34 (561, Biolegend), CD45RA (HI100, Biolegend), CD56 (HCD56, BD), CD62L (SK11, BD), CD66abce (TET2, Miltenyi Biotec), CD80 (L307.4, BD), CD86 (2331; BD); CD123 (7G3; BD), CD133 (7, Biolegend), CD141 (AD5-14H12; Miltenyi), CD146 (P1H12; Biolegend), HLA-DR (TU36, Life technologies), CCR2 (K036C2, Biolegend), CCR4 (L291H4, Biolegend), CCR6 (11A9, BD) and CCR7 (150503, BD) (Panels summarized in S3 Table ). Fixation was performed with 1% paraformaldehyde. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star).
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