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Sensiprot kit

Manufactured by Labtest
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Sourced in Brazil

The Sensiprot kit is a laboratory equipment product that is used for the detection and quantification of proteins. It provides a reliable and accurate method for measuring protein levels in various samples.

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Lab products found in correlation

Nephrat kit, by Exocell (2 mentions) Proteosilver plus kit, by Merck Group (1 mentions) Urea ce kit, by Labtest (1 mentions) Accu check performa meter, by Roche (1 mentions)

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Sensiprot assay kit

6 protocols using sensiprot kit

1

Comprehensive Renal Function Assessment

Cited 1 time
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Serum creatinine concentration was measured using the Jaffé method. The animals were classified as AKI according to the AKIN (Mehta et al. 2007 (link)), in which the diagnosis of AKI is defined by an increase in serum creatinine level above 150–200% from baseline.
Serum urea was measured by UV UREASI/GLDH kinetic. Urinary and serum sodium, potassium, chloride, urinary pH, and partial pressure of carbon dioxide (pCO2) in urine were measured on a Radiometer ABL800Flex (Radiometer Medical, Bronshoj, Demmark). Urinary bicarbonate concentration was calculated using the Handerson–Hasselbach equation. All these data were used to calculate the fractional excretion of ions. Urine protein excretion was quantified using a Sensiprot kit (Labtest, Minas Gerais, Brazil) and qualified by 10% SDS‐PAGE containing 10 μg of creatinine and 2 μg of bovine serum albumin (BSA) as a positive control, silver stained using a Proteosilver Plus kit (Sigma). Urinary glucose and creatinine were measured before ischemia (0), during ischemia (60 min), and post reperfusion (135, 360, 780, and 1080 min).
Malagrino P.A., Venturini G., Yogi P.S., Dariolli R., Padilha K., Kiers B., Gois T.C., da Motta‐Leal‐Filho J.M., Takimura C.K., Girardi A.C., Carnevale F.C., Zeri A.C., Malheiros D.M., Krieger J.E, & Pereira A.C. (2014). Catheter‐based induction of renal ischemia/reperfusion in swine: description of an experimental model. Physiological Reports, 2(9), e12150.
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2

Metabolic Cage Analysis of Rats

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In the last week of treatment protocol, the rats were placed in individual metabolic cages (Tecniplast 304) for 48 hours for the analysis of metabolic parameters. The first 24 hours were used for adaptation, and the following 24 hours were used to record food and water intake and to collect all the urine produced. NS, LS or HS chows were given ad libitum to the respective experimental groups. Urine produced in 24 h was measured by gravimetry and samples were immediately frozen at -20°C for posterior analysis. Creatinine concentration in blood and urine was determined by a kinetic method (Labtest, Minas Gerais—Brazil) based on the Jaffé reaction. The glomerular filtration rate (GFR) was estimated by the creatinine clearance. Sodium and potassium excretion was measured on a radiometer ABL800 Flex121 blood gas analyzer (Radiometer Medical, Brønshøj, Denmark). Protein excretion was determined using a spectrophotometric assay with a Sensiprot kit (Labtest, Minas Gerais—Brazil). The concentration of albumin in the urine samples was determined by using an ELISA kit (Nephrat kit Exocell, Philadelphia, PA). All kits were used following the manufacturer’s instructions.
Berger R.C., Vassallo P.F., Crajoinas R.D., Oliveira M.L., Martins F.L., Nogueira B.V., Motta-Santos D., Araújo I.B., Forechi L., Girardi A.C., Santos R.A, & Mill J.G. (2015). Renal Effects and Underlying Molecular Mechanisms of Long-Term Salt Content Diets in Spontaneously Hypertensive Rats. PLoS ONE, 10(10), e0141288.
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3

Protein Quantification Methods for Adherent Cells

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For experiments with adherent cells, protein content was determined using the Bradford method with bovine serum albumin as standard [30 (link)]. For experiments in supernatant of adherent cells, protein content was measured using the Sensiprot kit (Labtest Diagnostica, MG, Brazil).
Beckenkamp A., Willig J.B., Santana D.B., Nascimento J., Paccez J.D., Zerbini L.F., Bruno A.N., Pilger D.A., Wink M.R, & Buffon A. (2015). Differential Expression and Enzymatic Activity of DPPIV/CD26 Affects Migration Ability of Cervical Carcinoma Cells. PLoS ONE, 10(7), e0134305.
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4

Inflammatory Cell Profiling in BALF

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The total inflammatory cell count was realized from the BALF pellet using a neubauer chamber and the differential cells of the BALF were analyzed from cyto-centrifuged slides stained with the rapid panotic kit (Hematoxylin & Eosin) and on an optical microscope (100x objective). The protein content was measure from the BALF supernatant using the SENSIPROT kit (LabTest, Minas Gerais, MG, Brazil) and the test was carried out in accordance with the manufacturer's specifications.
Bernardo L.R., Ferreira L.K.D.P., Ferreira L.A.M.P., Vieira C.I.D., Oliveira J.B., Lima L.M., Alves A.F., Araújo R.S., Maia M.S., Scotti M.T., Barbosa Filho J.M, & Piuvezam M.R. (2022). Milonine attenuates the lipopolysaccharide-induced acute lung injury in mice by modulating the Akt/NF-κB signaling pathways. Anais da Academia Brasileira de Ciencias, 94(suppl 4).
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5

Comprehensive Renal Function Analysis

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Analysis of renal function was performed according to previously published studies (Silva-Aguiar et al., 2018 (link); Teixeira et al., 2020 (link); Peruchetti et al., 2021 (link); Farias et al., 2023 (link); Peres et al., 2023 (link)). Briefly, 24-h urine samples were quantified to determine urinary volume (mL) and urinary flow (mL/min). Urine samples were then centrifuged 5 times (10,000 × g for 10 min) to remove urine sediments. Plasma was obtained by centrifuging whole blood (2,500 × g for 5 min). All parameters analyzed in urine and plasma were measured using commercial kits following the manufacturers’ instructions. The levels of creatinine, blood urea nitrogen (BUN), and urinary proteinuria in both urine and plasma were measured, respectively, using the creatinine kit (ref. 35-100), urea CE kit (ref. 27-500), and sensiprot kit (ref. 36) purchased from Labtest (Lagoa Santa, MG, Brazil). The levels of urinary and plasma sodium were measured using the enzymatic sodium kit (ref. BT1201100) purchased from BioTecnica (Varginha, MG, Brazil). These results were then utilized to calculate parameters such as creatinine clearance (CCr), sodium clearance, renal fractional excretion of sodium (FENa+), and urinary protein to creatinine ratio (UPCr).
Peres R.A., Peruchetti D.B., Silva-Aguiar R.P., Teixeira D.E., Gomes C.P., Takiya C.M., Pinheiro A.A, & Caruso-Neves C. (2023). Rapamycin treatment induces tubular proteinuria: role of megalin-mediated protein reabsorption. Frontiers in Pharmacology, 14, 1194816.
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6

Evaluating Metabolic Markers in Rats

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Fasting glucose levels were measured using the ACCU-CHECK® Performa meter (Roche Diagnostics GmbH, Mannheim, Germany). The serum urea was measured by colorimetric enzymatic hydrolysis by the urease system using a Labtest kit (Labtest, Minas Gerais, Brazil). Serum and urinary creatinine concentrations were measured using a kinetic method (Labtest) and a ThermoPlate Analyzer Plus (ThermoPlate, São Paulo, Brazil). The urinary protein excretion was determined using a Sensiprot kit (Labtest). The urinary albumin concentration was determined using an ELISA kit specific for rat urine albumin (Nephrat kit; Exocell, Philadelphia, PA, USA). The experiments were carried out in accordance with the manufacturer’s instructions.
Beraldo J.I., Benetti A., Borges-Júnior F.A., Arruda-Junior D.F., Martins F.L., Jensen L., Dariolli R., Shimizu M.H., Seguro A.C., Luchi W.M, & Girardi A.C. (2019). Cardioprotection Conferred by Sitagliptin Is Associated with Reduced Cardiac Angiotensin II/Angiotensin-(1-7) Balance in Experimental Chronic Kidney Disease. International Journal of Molecular Sciences, 20(8), 1940.
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