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Gatan imaging filter

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The Gatan Imaging Filter is a high-performance electron energy filter used in transmission electron microscopy (TEM) applications. It is designed to improve the quality and resolution of TEM images by selectively filtering electrons based on their energy.

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Lab products found in correlation

K3 direct electron detector, by Ametek (4 mentions) Volta phase plate, by Ametek (3 mentions) Krios g3i 300 kev, by Thermo Fisher Scientific (2 mentions) Alpha 300r, by WITec (1 mentions) Spectrum 100 ftir spectrometer, by PerkinElmer (1 mentions) Advance powder x ray diffractometer, by Bruker (1 mentions) Krios g3i, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Imaging Filter (5 citations) Gatan Quantum-LS imaging energy filter (2 citations)

5 protocols using gatan imaging filter

1

Multimodal Characterization of Advanced Materials

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STEM-EDX was performed using a JEOL 2100 LaB TEM microscope equipped with an Oxford X-Max 800 mm2 EDX detector from Oxford Instruments. STEM-EELS was carried out using an uncorrected FEI TITAN microscope operating at 300 kV. The microscope was equipped with the Gatan Imaging Filter (Gatan Inc., CA, USA). Typically, EELS spectra were acquired with a dispersion of 0.1 eV per channel with an energy resolution of ∼1 eV (FWHM of the zero-loss peak) and an entrance aperture of 2 mm on the spectrometer. FT-IR spectra were acquired using a PerkinElmer Spectrum 100 FT-IR spectrometer in the range 4000–550 cm−1. Powder XRD was performed using a Bruker Advance Powder X-ray diffractometer with a molybdenum K-α emission source in the Bragg–Brentano configuration. XPS measurements were obtained using an Omicron EA 125 Energy Analyser with a monochromated Al K-α source at 1486.7 eV, where the binding energy was calibrated to the C 1s peak. High resolution core level component XPS scans were obtained with a pass energy of 20 eV, high magnification mode, entrance and exit slits of 6 mm and 3 mm respectively giving an overall total resolution of 0.6 eV with an instrument resolution of 0.46 eV. Raman spectra for this work were acquired using a WITec Alpha 300R with a 532 nm excitation source at 1 mW with 1800 lines per mm spectral grating.
Tyndall D., Craig M.J., Gannon L., McGuinness C., McEvoy N., Roy A., García-Melchor M., Browne M.P, & Nicolosi V. (2023). Demonstrating the source of inherent instability in NiFe LDH-based OER electrocatalysts. Journal of Materials Chemistry. a, 11(8), 4067-4077.
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2

Cryo-Electron Tomography of Cellular Ultrastructure

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Cryo-ET was performed on a ThermoFisher Krios G3i 300 keV field emission gun cryo-TEM. Dose-fractionated Imaging was performed using the SerialEM software57 (link) on a K3 direct electron detector58 (link) (Gatan Inc., Pleasanton, CA, USA) operated in the electron-counted mode. Motion correction of images was done using the Alignframe function in SerialEM. We additionally used the Volta phase plate59 (link),60 (link) to increase contrast without high defocus, and the Gatan Imaging Filter (Gatan Inc., Pleasanton, CA, USA) with a slit width of 20 eV to increase contrast by removing inelastically scattered electrons61 (link). After initially assessing cells at lower magnifications for suitability of ice thickness and plasma membrane integrity, tilt series were collected with a span of 120° (−60° to +60°; bi-directional scheme) with 2° increments at a magnification of 33,000X (with a corresponding pixel size of 2.65 Å) and a defoci range of −1 to −3 µm. The cumulative dose of each tilt-series ranged between 100 and 150 e−/Å2. Once acquired, tilt series were aligned using the 10 nm colloidal gold as fiducials and reconstructed into tomograms by our in-house automated computation pipeline utilizing the IMOD software package62 (link).
Mageswaran S.K., Guérin A., Theveny L.M., Chen W.D., Martinez M., Lebrun M., Striepen B, & Chang Y.W. (2021). In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems. Nature Communications, 12, 4983.
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3

Cryo-ET Workflow for High-Resolution Imaging

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Cryo-ET was performed on a Thermo Fisher Krios G3i 300 keV field emission cryo-transmission electron microscope. Dose-fractionated imaging was performed using the SerialEM software41 (link) on a K3 direct electron detector42 (link) (Gatan Inc., Pleasanton, CA, USA) operated in electron-counted mode. Motion correction of images was done using the Alignframe function in IMOD43 (link). Imaging was done using a Volta phase plate44 (link) to increase contrast without high defocus, and the Gatan Imaging Filter (Gatan Inc., Pleasanton, CA, USA) with a slit width of 20 eV to increase contrast by removing inelastically scattered electrons45 (link). After initially assessing cells at lower magnifications for suitability of ice thickness and plasma membrane integrity, tilt series were collected with a span of 100° (-50° to +50°; dose-symmetric scheme46 ) or 120° (-60° to +60°; dose-symmetric or bi-directional scheme) with 2° increments at a magnification of 33,000x (with a corresponding pixel size of 2.65 Å) and a defocus range of -1 to -4 μm. Each tilt series was collected with a cumulative dose of around 140 e−/ Å2 (link). Once acquired, tilt series were aligned using the 10 nm colloidal gold as fiducials and reconstructed into tomograms by our in-house automated computation pipeline utilizing the IMOD software package43 (link).
Martinez M., Chen W.D., Cova M.M., Molnár P., Mageswaran S.K., Guérin A., John A.R., Lebrun M, & Chang Y.W. (2022). Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. Nature microbiology, 7(8), 1230-1238.
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4

Cryo-ET Imaging of Biological Samples

Cited 1 time
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Cryo-ET was performed on a Thermo Fisher Krios G3i field-emission transmission electron microscope operating at 300 kV. Dose-fractionated imaging was performed using the SerialEM software 83 (link) on a K3 direct electron detector 84 (link) (Gatan Inc., Pleasanton, CA, USA) operated in electron-counting mode. Motion correction of images was done using Alignframes in IMOD 85 (link). Additionally, we used the Gatan Imaging Filter (Gatan Inc., Pleasanton, CA, USA) with a slit width of 20 eV to increase contrast by removing inelastically scattered electrons 86 (link). Tilt series were collected with a span of 120° (−60° to +60°; dose-symmetric scheme) with 3° increments at a magnification of 53,000X (with a corresponding pixel size of 1.68 Å) and a defocus range of −4 to −6 μm using the fast-incremental single exposure method 87 (link). The cumulative dose of each tilt series ranged between 90 and 110 e−/Å2. Once acquired, tilt series were aligned using the 10 nm colloidal gold as fiducials. Afterwards, the 10 nm colloidal gold was computationally erased to facilitate downstream data analysis. Tilt series were dose-weighted and CTF-corrected by the IMOD software package 85 (link). Processed tilt series were reconstructed into 3D tomograms using weighted back-projection and a simultaneous iterative reconstruction technique (SIRT)-like filter.
Gorbea Colón J.J., Palao L I.I.I., Chen S.F., Kim H.J., Snyder L., Chang Y.W., Tsai K.L, & Murakami K. (2023). Structural basis of a transcription pre-initiation complex on a divergent promoter. Molecular cell, 83(4), 574-588.e11.
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5

Cryo-ET Workflow for High-Resolution Imaging

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Cryo-ET was performed on a Thermo Fisher Krios G3i 300 keV field emission cryo-transmission electron microscope. Dose-fractionated imaging was performed using the SerialEM software41 (link) on a K3 direct electron detector42 (link) (Gatan Inc., Pleasanton, CA, USA) operated in electron-counted mode. Motion correction of images was done using the Alignframe function in IMOD43 (link). Imaging was done using a Volta phase plate44 (link) to increase contrast without high defocus, and the Gatan Imaging Filter (Gatan Inc., Pleasanton, CA, USA) with a slit width of 20 eV to increase contrast by removing inelastically scattered electrons45 (link). After initially assessing cells at lower magnifications for suitability of ice thickness and plasma membrane integrity, tilt series were collected with a span of 100° (-50° to +50°; dose-symmetric scheme46 ) or 120° (-60° to +60°; dose-symmetric or bi-directional scheme) with 2° increments at a magnification of 33,000x (with a corresponding pixel size of 2.65 Å) and a defocus range of -1 to -4 μm. Each tilt series was collected with a cumulative dose of around 140 e−/ Å2 (link). Once acquired, tilt series were aligned using the 10 nm colloidal gold as fiducials and reconstructed into tomograms by our in-house automated computation pipeline utilizing the IMOD software package43 (link).
Martinez M., Chen W.D., Cova M.M., Molnár P., Mageswaran S.K., Guérin A., John A.R., Lebrun M, & Chang Y.W. (2022). Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. Nature microbiology, 7(8), 1230-1238.
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