Western blot analysis was performed as described previously 15 (link). Briefly, equal amount of proteins were resolved by SDS-PAGE and transferred to PVDF membranes (Immobilon-P, Millipore Corp). Membranes were incubated with optimal concentrations of relevant primary antibody and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or -rabbit secondary antibody (Sigma), washed 3×5 min in TBS-T and visualized by Western Lightning Plus-ECL according to manufacturer's protocol (Perkin Elmer). The antibodies used were mouse Noxa (114C307) (Calbiochem), rabbit anti-Noxa (FL-54), anti-κBα (C-21) and mouse anti-NF-κB p65 (F-6) (Santa Cruz Biotechnology), rabbit anti-Lamin A/C and mouse HSP27 (Cell Signaling Technology), rabbit anti-p-Ser 15, 78, or 82 HSP27 (Stressgen), anti-K48-linkage Specific Polyubiquitin mAB (Cell Signaling), mouse anti-β-Actin, and anti-β-tubulin (Sigma).
Horseradish peroxidase hrp conjugated anti mouse or rabbit secondary antibody
Manufactured by Merck Group
Horseradish peroxidase (HRP)-conjugated anti-mouse or -rabbit secondary antibody is a laboratory reagent used in various immunoassay techniques. It is a detection antibody that binds to primary antibodies raised in mouse or rabbit, and the conjugated HRP enzyme can be used to generate a colorimetric or chemiluminescent signal, allowing the visualization and quantification of target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Immobilon p, by Merck Group (2 mentions)
Anti β tubulin, by Merck Group (2 mentions)
Rabbit anti lamin a c and mouse hsp27, by Cell Signaling Technology (2 mentions)
Anti k48 linkage specific polyubiquitin mab, by Cell Signaling Technology (2 mentions)
Mouse anti β actin, by Merck Group (2 mentions)
Western lightning plus ecl, by PerkinElmer (2 mentions)
2 protocols using horseradish peroxidase hrp conjugated anti mouse or rabbit secondary antibody
1
Western Blot Protein Analysis Protocols
2
Western Blot Protein Analysis Protocols
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Western blot analysis was performed as described previously 15 (link). Briefly, equal amount of proteins were resolved by SDS-PAGE and transferred to PVDF membranes (Immobilon-P, Millipore Corp). Membranes were incubated with optimal concentrations of relevant primary antibody and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or -rabbit secondary antibody (Sigma), washed 3×5 min in TBS-T and visualized by Western Lightning Plus-ECL according to manufacturer's protocol (Perkin Elmer). The antibodies used were mouse Noxa (114C307) (Calbiochem), rabbit anti-Noxa (FL-54), anti-κBα (C-21) and mouse anti-NF-κB p65 (F-6) (Santa Cruz Biotechnology), rabbit anti-Lamin A/C and mouse HSP27 (Cell Signaling Technology), rabbit anti-p-Ser 15, 78, or 82 HSP27 (Stressgen), anti-K48-linkage Specific Polyubiquitin mAB (Cell Signaling), mouse anti-β-Actin, and anti-β-tubulin (Sigma).
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