Tissue microarrays (TMAs) were constructed using appropriate tissue cores from formalin-fixed and paraffin-embedded samples as described previously [20 ]. Briefly, the appropriate tumor areas and corresponding non-tumor gastric samples were selected by pathologists, and a single core (diameter 0.6 mm) was taken from each tissue. TMA blocks were constructed using an automated tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA). The array blocks were cut into 5-μm sections, and the sections were stained with hematoxylin and eosin to verify the presence of tumor cells. In all cases, tissue cores obtained from normal adjacent tissue served as internal controls.
Automated tissue arrayer
Manufactured by Beecher Instruments
Sourced in United States
The Automated tissue arrayer is a laboratory instrument designed to precisely extract and arrange small tissue samples from donor tissue blocks onto a recipient paraffin block. The device automates the process of creating tissue microarrays for high-throughput analysis and research.
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Lab products found in correlation
7 protocols using automated tissue arrayer
1
Tissue Microarray Construction for Gastric Cancer
2
Pancreatic Cancer Tissue Microarray Protocol
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Pancreatic cancer samples with informed consent were collected between 1995 and 2009 from 90 patients who underwent pancreatic surgery and were were stored at Biobank Center of National Engineering Center for Biochip at Shanghai. All patients including complete clinical data and adequate tissue in this study were identified. Ethical approval for the study was obtained from the ethical committee of biobank center related hospitals.
Original formalin-fixed, paraffin-embedded specimens were used to construct a PDAC tissue microarray (FFPE TMA). Hematoxylin and Eosin (H&E)-stained standard slides from each tumor specimen were reviewed by a single pathologist (MR), who was blinded to specimen protein expression status. Representative tumor regions and its paracancerous nonmalignant pancreatic specimens (NMPs) were selected from each tissue block and 2 tissue cores (0.6 mm in diameter) were taken from each region using an automated tissue arrayer (Beecher Instruments, Sun Prarie, WI). Cores were transferred to individual recipient blocks. In all cases, cores were taken normal adjacent pancreas were also used as internal controls. Five-micron sections were cut from each recipient block. Sections were stained with H&E to confirm the presence of tumor within each core.
Original formalin-fixed, paraffin-embedded specimens were used to construct a PDAC tissue microarray (FFPE TMA). Hematoxylin and Eosin (H&E)-stained standard slides from each tumor specimen were reviewed by a single pathologist (MR), who was blinded to specimen protein expression status. Representative tumor regions and its paracancerous nonmalignant pancreatic specimens (NMPs) were selected from each tissue block and 2 tissue cores (0.6 mm in diameter) were taken from each region using an automated tissue arrayer (Beecher Instruments, Sun Prarie, WI). Cores were transferred to individual recipient blocks. In all cases, cores were taken normal adjacent pancreas were also used as internal controls. Five-micron sections were cut from each recipient block. Sections were stained with H&E to confirm the presence of tumor within each core.
3
Tissue Microarray Construction for Liver Cancer
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Liver cancer TMAs were constructed using tissue cores from formalin-fixed, paraffin-embedded specimens, as previously described (14 (link)). A pathologist was responsible for reviewing the hematoxylin and eosin (H&E)-stained slides to determine tumor location. Representative tumor regions and their paracancerous tissues containing 2 tissue cores in each region were taken using an automated tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA). Cores were transferred to individual recipient blocks. Sections (5 µm) were cut from each recipient block to confirm the presence of the tumor.
4
Placental Tissue Microarray Analysis
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Tissue microarrays were constructed from central tissue blocks of third trimester FFPE placentas (n = 100) as described earlier (337 (link)). Briefly, three 20 × 35 mm recipient blocks were made of Paraplast X-Tra tissue embedding media (Fisher Scientific, Pittsburgh, PA, USA). One mm diameter cores from tissue blocks were transferred in triplicate into recipient paraffin blocks using an automated tissue arrayer (Beecher Instruments, Inc., Silver Spring, MD, USA). Five µm sections cut from TMAs were placed on silanized slides and stained with antibodies and reagents (Data S18 in Supplementary Material) either on Ventana Discovery or Leica BOND-MAX autostainers.
Tissue microarray immunostainings were semi-quantitatively scored by three examiners blinded to the clinical information with an immunoreactive score modified from a previously published one (154 (link)). Immunostaining intensity was graded as follows: 0 = negative, 1 = weak, 2 = intermediate, and 3 = strong. All villi in a random field of each of the three cores were evaluated by all examiners, and scores within each core were averaged to represent the target protein quantity of that core. Thus, each placenta had three scores corresponding to the three cores examined.
Tissue microarray immunostainings were semi-quantitatively scored by three examiners blinded to the clinical information with an immunoreactive score modified from a previously published one (154 (link)). Immunostaining intensity was graded as follows: 0 = negative, 1 = weak, 2 = intermediate, and 3 = strong. All villi in a random field of each of the three cores were evaluated by all examiners, and scores within each core were averaged to represent the target protein quantity of that core. Thus, each placenta had three scores corresponding to the three cores examined.
5
Gallbladder Cancer Tissue Microarrays
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The GBC tissue microarrays were constructed using tissue cores from Formalin-fixed, paraffin-embedded specimens. Representative cancer tissue regions and para-cancerous non-malignant or non-premalignant lesions gallbladder specimens were selected from each tissue block by licensed pathologists, and a single 1.5-mm core was taken from every donor block. Microarray blocks were constructed using an automated tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA). Five-micrometer sections were dissected from the array blocks. Sections were stained with hematoxylin and eosin (H&E) to confirm the presence of tumor in each core.
6
Tissue Microarray Construction from FFPE Samples
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Tissue microarrays were constructed using appropriate tissue cores from formalin-fixed and paraffin-embedded samples as described previously [37 (link)]. Briefly, appropriate tumor areas and corresponding non-tumor gastric samples were selected by pathologists and a single core (diameter 0.6 mm) was taken from each tissue. Tissue microarray blocks were constructed using an automated tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA). The array blocks were cut into 5-μm sections, and the sections were stained with hematoxylin and eosin to verify the presence of tumor cells. In all cases, tissue cores obtained from normal adjacent tissue served as internal controls.
7
Tissue Microarray Construction Protocol
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Tissue microarrays were constructed using appropriate tissue cores from formalin-fixed and paraffin- embedded samples as described previously [18 (link)]. Briefly, appropriate tumor areas and corresponding non-tumor gastric samples were selected by pathologists and a single core (diameter 0.6 mm) was taken from each tissue. Tissue microarray blocks were constructed using an automated tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA). The array blocks were cut into 5-μm sections, and the sections were stained with hematoxylin and eosin to verify the presence of tumor cells. In all cases, tissue cores obtained from normal adjacent tissue served as internal controls.
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