Log In Sign up
The largest database of trusted experimental protocols

Anti mef2c antibody

Manufactured by Cell Signaling Technology
Visit Supplier

The Anti-Mef2c antibody is a laboratory tool designed for the detection and analysis of the Mef2c protein. Mef2c is a transcription factor that plays a crucial role in the regulation of gene expression during cellular development and differentiation. The antibody can be used for various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of Mef2c in different cell types and tissues.

Automatically generated - may contain errors

Lab products found in correlation

Anti gapdh antibody, by Merck Group (1 mentions) Anti gfp antibody, by Thermo Fisher Scientific (1 mentions) Anti myc, by Thermo Fisher Scientific (1 mentions) Anti mcherry antibody, by Abcam (1 mentions) Anti gata4 antibody, by Santa Cruz Biotechnology (1 mentions) Anti ty1 antibody, by Diagenode (1 mentions) Anti flag, by Merck Group (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions) Complete protease inhibitor cocktail tablet, by Roche (1 mentions) Mab374, by Merck Group (1 mentions) Falcon cultureslides, by BD (1 mentions) Biotinylated anti rabbit igg, by Vector Laboratories (1 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Simplechip enzymatic chromatin ip kit, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

MEF2C (22 citations) Anti-MEF2C (4 citations)

4 protocols using anti mef2c antibody

1

Comprehensive Western Blot Analysis Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analyses were performed as previously described3 (link). Briefly, cell lysates were prepared using RIPA buffer with complete phosphatase and protease inhibitor cocktail tablets (Roche) and boiled in Laemmli buffer for 5 min at 95 °C. Antibodies utilized were anti-Mef2c antibody (1:1,000; Cell Signaling, 5030), anti-Gata4 antibody (1:500; Santa Cruz, sc25310), anti-Tnnt2 antibody (1:500; Thermo Scientific, MA5–12960), anti-GFP antibody (1:500; Thermo Scientific, A-11122), anti-PHF7 antibody (1:500; LSBio, B11090), anti-Ty1 antibody (1:1,000; Diagenode, C15200054), anti-Myc (1:1,000; Invitrogen, 46–0603), anti-Flag (1:1,000; Sigma, F7425), anti-mCherry antibody (1:1,000; Abcam, ab167453) and anti-GAPDH antibody (1:1,000; Merck Millipore, MAB374).
Garry G.A., Bezprozvannaya S., Chen K., Zhou H., Hashimoto H., Morales M.G., Liu N., Bassel-Duby R, & Olson E.N. (2021). The histone reader PHF7 cooperates with the SWI/SNF complex at cardiac super enhancers to promote direct reprogramming. Nature cell biology, 23(5), 467-475.
+ Open protocol
+ Expand
2

Protein Expression Analysis in HEK-293 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK-293 cells were transfected with plasmids using FuGENE6 and cell lysates were analyzed after 48 hours of transfection. Western blot analyses were performed as previously described (Song et al., 2012 (link)). Briefly, cell lysates were prepared using RIPA buffer with complete protease inhibitor cocktail tablets (Roche). Lysates were boiled with 4x Laemmli buffer for 5 min at 95°C. Antibodies used were anti-Mef2c antibody (1:1000, Cell Signaling, 5030), anti-Hand2 antibody (1:200, R&D Systems, AF3876), anti-Ty1 antibody (1:1000, Diagenode, C15200054), and anti-Gapdh antibody (1:1000, Merck Millipore, MAB374).
Hashimoto H., Wang Z., Garry G.A., Malladi V.S., Botten G.A., Ye W., Zhou H., Osterwalder M., Dickel D.E., Visel A., Liu N., Bassel-Duby R, & Olson E.N. (2019). Cardiac Reprogramming Factors Synergistically Activate Genome-wide Cardiogenic Stage-Specific Enhancers. Cell stem cell, 25(1), 69-86.e5.
+ Open protocol
+ Expand
3

Immunofluorescence Staining of MEF2C

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vitro cultures, cells were seeded in BD Falcon CultureSlides (BD Biosciences) and cultured with M-CSF and RANKL. For sorted cells by flow cytometry, cells were immobilized on slide glasses with Cytospin. Cells were fixed with 4% formaldehyde for 10 min and then blocked with 5%FBS/0.3%TritonX-100/PBS for 60 min. Anti-MEF2C antibody (Cell Signaling Technology) was added to cells in 1:400 dilution with 1%BSA/0.3%TritonX-100/PBS and incubated overnight at 4 °C. After washing with PBS, 1:1 000 diluted Alexa Fluor 488 anti-rabbit IgG (Thermo Scientific) or 1:300 diluted Biotinylated anti-rabbit IgG (Vector Laboratories) were incubated in 1%BSA/0.3%TritonX-100/PBS for 60 min at room temperature. Cells incubated with Biotinylated anti-rabbit IgG were stained with 1:500 diluted streptavidin Alexa Fluor 594 for 30 min at room temperature. Cells were mounted with Fluoroshield Mounting Medium and observed using NIKON SMZ25 with CMOS camera (Zyla 5.5, Andor). Fluorescence intensity was evaluated with ImageJ v1.52a.
Fujii T., Murata K., Mun S.H., Bae S., Lee Y.J., Pannellini T., Kang K., Oliver D., Park-Min K.H, & Ivashkiv L.B. (2021). MEF2C regulates osteoclastogenesis and pathologic bone resorption via c-FOS. Bone Research, 9, 4.
+ Open protocol
+ Expand
4

Chromatin Immunoprecipitation (ChIP) Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chromatin immunoprecipitation (ChIP) assays were performed with a Simplechip Enzymatic Chromatin IP kit (Cell Signaling Technology, #9003 S) following the product manual. Briefly, isolated splenocytes were crosslinked with 1% formaldehyde (Sigma, #47608-250ML-F). After cell lysis, nuclei were extracted and subjected to sonication for chromatin fragmentation. Chromatin samples were incubated for 4 h with rotation at 4 °C after adding anti-MEF2C antibody (Cell Signaling Technology, #5030 S). Antibody–chromatin complexes were pulled down by magnetic protein A/G beads. Purified DNAs were quantified by real-time PCR. Primers are listed in the Supplementary Table 1.
Li X., Poire A., Jeong K.J., Zhang D., Ozmen T.Y., Chen G., Sun C, & Mills G.B. (2024). C5aR1 inhibition reprograms tumor associated macrophages and reverses PARP inhibitor resistance in breast cancer. Nature Communications, 15, 4485.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!