Log In Sign up
The largest database of trusted experimental protocols

Thiol modified dna oligonucleotides

Manufactured by Integrated DNA Technologies
Visit Supplier

Thiol-modified DNA oligonucleotides are single-stranded DNA molecules with a thiol (-SH) functional group attached. The thiol group allows for the covalent attachment of the oligonucleotide to various surfaces or molecules. These modified oligonucleotides are commonly used in applications such as surface immobilization, conjugation, and labeling.

Automatically generated - may contain errors

Lab products found in correlation

Tris 2 carboxyethyl phosphine, by Merck Group (3 mentions) Iodixanol gradients, by Cosmo Bio (3 mentions) 18 1 mpb pe, by Avanti Polar Lipids (2 mentions) Octyl β d glucopyranoside og, by Merck Group (1 mentions) Mpb pe, by Avanti Polar Lipids (1 mentions)

3 protocols using thiol modified dna oligonucleotides

1

Lipid-Oligonucleotide Conjugation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thiol-modified DNA oligonucleotides (Integrated DNA Technologies) were treated with tris(2-carboxyethyl)phosphine (Sigma-Aldrich) for 30 minutes (50 nmole DNA per reaction) and immediately reacted with 1 µmole of 18:1 MPB PE (Avanti Polar Lipids) in aqueous solutions containing ~2% Octyl β-D-glucopyranoside (OG, purchased from EMD Millipore) at room temperature for 30 minutes. Such a reaction mixture was then diluted ~3-fold with OG concentration brought up to 4%, added to 2.5 µmole of pre-dried DOPC, agitated, and dialyzed overnight. The conjugation products were separated from unconjugated DNA via isopycnic centrifugation in iodixanol gradients (Cosmo Bio USA) and later analyzed by SDS-polyacrylamide gel electrophoresis.
Zhang Z., Yang Y., Pincet F., Llaguno M, & Lin C. (2017). Placing and shaping liposomes with reconfigurable DNA nanocages. Nature chemistry, 9(7), 653-659.
+ Open protocol
+ Expand
2

Lipidation of Thiol-modified DNA Oligonucleotides

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thiol-modified DNA oligonucleotides (Integrated DNA Technologies) were treated with tris(2-carboxyethyl)phosphine (Sigma-Aldrich) for 30 minutes (20 nmole DNA per reaction) and immediately reacted with 0.4 μmole of 18:1 MPB PE (Avanti Polar Lipids) in aqueous solutions containing ~2% OG (EMD Millipore) at room temperature for 45 minutes. Such a reaction mixture was then diluted 2-fold, added to 0.6 μmole of pre-dried lipids, agitated and dialyzed overnight in order to incorporate lipidated DNA molecules into liposomes. The conjugation products were separated from unconjugated DNA via isopycnic centrifugation in iodixanol gradients (Cosmo Bio USA) and later analyzed by SDS-polyacrylamide gel electrophoresis.
Yang Y., Wang J., Shigematsu H., Xu W., Shih W.M., Rothman J.E, & Lin C. (2016). Self-assembly of size-controlled liposomes on DNA nanotemplates. Nature chemistry, 8(5), 476-483.
+ Open protocol
+ Expand
3

Lipidation of Thiol-modified DNA Oligonucleotides

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thiol-modified DNA oligonucleotides (Integrated DNA Technologies) were treated with tris(2-carboxyethyl)phosphine (Sigma-Aldrich) for 30 minutes (20 nmole DNA per reaction) and immediately reacted with 0.4 μmole of 18:1 MPB PE (Avanti Polar Lipids) in aqueous solutions containing ~2% OG (EMD Millipore) at room temperature for 45 minutes. Such a reaction mixture was then diluted 2-fold, added to 0.6 μmole of pre-dried lipids, agitated and dialyzed overnight in order to incorporate lipidated DNA molecules into liposomes. The conjugation products were separated from unconjugated DNA via isopycnic centrifugation in iodixanol gradients (Cosmo Bio USA) and later analyzed by SDS-polyacrylamide gel electrophoresis.
Yang Y., Wang J., Shigematsu H., Xu W., Shih W.M., Rothman J.E, & Lin C. (2016). Self-assembly of size-controlled liposomes on DNA nanotemplates. Nature chemistry, 8(5), 476-483.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!