Quanta 250 feg sem

Manufactured by Thermo Fisher Scientific

Sourced in United States, Netherlands, United Kingdom, Germany, Switzerland

The Quanta 250 FEG SEM is a field emission gun scanning electron microscope. It provides high-resolution imaging and analysis of a wide range of materials and samples.

Automatically generated - may contain errors

Lab products found in correlation

Etd bse, by Thermo Fisher Scientific (8 mentions) Paraformaldehyde (pfa), by Merck Group (3 mentions) Glutaraldehyde, by Merck Group (3 mentions) Jem 2100, by JEOL (2 mentions) Em ace 600 high vacuum sputterer, by Leica (2 mentions) Escalab 250xi, by Thermo Fisher Scientific (2 mentions) Hepes buffer, by Merck Group (2 mentions) K850 critical point drier cpd, by Quorum Technologies (2 mentions) Sc7620 mini sputter coater, by Quorum Technologies (2 mentions) Thincert well inserts, by Greiner (2 mentions) Tristar 2 3020, by Micromeritics (1 mentions) Vertex 70, by Bruker (1 mentions) Senterra raman microscope, by Bruker (1 mentions) 2100 hrtem, by JEOL (1 mentions) Ethanol, by Merck Group (1 mentions) Xrdynamic500, by Anton Paar (1 mentions) Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions) Ftir spectrometer, by Thermo Fisher Scientific (1 mentions) Tecnai f30 electron microscope, by Thermo Fisher Scientific (1 mentions) Inspect s50 sem, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Quanta 250 FEG (630 citations) Quanta 250 (415 citations) FEG 250 (46 citations) Quanta 250 SEM (44 citations) FEG-SEM (6 citations) FE-SEM QUANTA 250 FEG (4 citations) SEM Model Quanta 250 FEG (3 citations) FEI QUANTA 250 FEG SEM microscope (2 citations)

83 protocols using quanta 250 feg sem

Morphology and Composition Analysis of Nanostructured LFS Coatings

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphology of the nanostructured LFS-coating was imaged using scanning electron microscopy (SEM). Prior to imaging, the samples were sputter coated with a thin layer of gold to reduce surface charging. Cross-sectional images of the coating layer were recorded using a Zeiss Sigma 300 VP SEM and top-view images using a FEI Quanta 250 FEG SEM. To determine particle diameters of the colloidal probes, low-vacuum SEM (LV-SEM) images were recorded using the same FEI Quanta 250 FEG SEM operating under low vacuum (70 Pa). No surface coating was used in these cases.
X-ray photoelectron spectroscopy (XPS) analysis was performed using an AXIS Ultra^DLD X-ray photoelectron spectrometer (Kratos Analytical) equipped with a monochromatic Al X-ray source. The analysis area was below 1 mm², with most of the signal coming from an area of about 700 × 300 μm². A wide spectrum was recorded to detect elements present in the surface layer. The relative surface compositions were obtained from detailed spectra recorded for each element and quantified using atomic sensitivity factors supplied by Kratos. A high-resolution carbon C1s spectrum was curve-fitted to identify different functional groups from the chemical shifts in the carbon signal.

Eriksson M., Claesson P.M., Järn M., Wallqvist V., Tuominen M., Kappl M., Teisala H., Vollmer D., Schoelkopf J., Gane P.A., Mäkelä J.M, & Swerin A. (2023). Effects of liquid surface tension on gas capillaries and capillary forces at superamphiphobic surfaces. Scientific Reports, 13, 6794.

+ Open protocol

+ Expand

Comprehensive Structural Analysis of Prepared Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols

The structures of the prepared materials were examined using a transmission electron microscope HRTEM (JEOL JEM 2100, JEOL, Tokyo, Japan), and morphologies were checked using the scanning electron microscope SEM (FEI-Quanta FEG-250 SEM, Basel, Switzerland). The phase identification and crystallinity were analyzed using X-ray diffraction (PANalytical Empyrean, Eindhoven, Netherlands) with CuKa radiation (wavelength 1.54045 Å), accelerating voltage of 40 KV and a current of 35 mA. Fourier transformation infrared spectroscopy (FTIR) was performed to obtain information about the intercalation of LDH using Vertex 70 (Bruker, Berlin, Germany). Raman spectroscopy (FT-Raman) of the as-prepared composites was obtained with Bruker Senterra Raman Microscope (Bruker Optics Inc., Germany). The BET surface area and pore size distribution were determined using a surface area analyzer (TriStar II 3020, Micromeritics, Norcross, GA, USA).

Abdelrazek G.M., EL-Deeb M.M., Farghali A.A., Pérez-Cadenas A.F, & Abdelwahab A. (2021). Design of Self-Supported Flexible Nanostars MFe-LDH@ Carbon Xerogel-Modified Electrode for Methanol Oxidation. Materials, 14(18), 5271.

+ Open protocol

+ Expand

Characterization of TiO2 Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The crystalline phase of
TiO₂ was confirmed and determined with X-ray diffraction
using an APEX II ultra-diffractometer with Cu Kα radiation at
λ = 1.54056 Å. To determine the primary TiO₂ particle size, an aqueous suspension of 0.05 g/L was sonicated with
a probe sonicator for 60 s with 15 s rest over 30 min in a room temperature
water bath. Afterward, a 15 μL aliquot was drop-cast onto a
formvar/carbon-coated 100 mesh copper grid and dried. The copper grid
was imaged using an 80 kV JEOL-1400 Plus transmission electron microscope.
Particle sizes were analyzed using ImageJ software for more than 100
particles. For scanning electron microscopy (SEM) images for particle
film morphology, 2.5 mg of the particles was sonicated in 700 μL
of water for 30 s. Then, fourteen 5 mm x 5 mm silicon wafers were
laid on the ATR crystal, and the colloidal suspension was pipetted
into the trough. The solution was dried, and a wafer was imaged using
a FEI Quanta FEG 250 SEM at 10 kV.
The specific surface area
was determined using a Quantachrome Nova 4200e N₂ adsorption
isotherm under liquid nitrogen. Samples were first degassed at 120
°C for 18 h, and a 15-multipoint isotherm was collected between
P/P₀ of 0.05–0.95.

Sit I., Quirk E., Hettiarachchi E, & Grassian V.H. (2022). Differential Surface Interactions and Surface Templating of Nucleotides (dGMP, dCMP, dAMP, and dTMP) on Oxide Particle Surfaces. Langmuir, 38(49), 15038-15049.

+ Open protocol

+ Expand

SEM Characterization of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

SEM characterizations
on the samples were carried out with a FEI Quanta FEG-250 SEM after
optical measurements. An acceleration voltage of 2 or 5 kV was applied
during the measurements to reduce charging on the sample surface.

Steele J.A., Yuan H., Tan C.Y., Keshavarz M., Steuwe C., Roeffaers M.B, & Hofkens J. (2017). Direct Laser Writing of δ- to α-Phase Transformation in Formamidinium Lead Iodide. ACS Nano, 11(8), 8072-8083.

+ Open protocol

+ Expand

SEM Examination of Gluten Fermentation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The structure of WBDF-gluten fermentation samples was examined by SEM. All samples were sputtered for gold plating in a sputtering coater and transferred to a Quanta FEG-250 SEM (FEI, Hillsboro, OR, USA) for examination at an accelerated voltage of 20 kV.

Wang Z., Ma S., Li L, & Huang J. (2022). Effect of wheat bran dietary fiber on structural properties and hydrolysis behavior of gluten after synergistic fermentation of Lactobacillus plantarum and Saccharomyces cerevisiae. Frontiers in Nutrition, 9, 982878.

+ Open protocol

+ Expand

Scanning Electron Microscopy of Rice Noodles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The samples were then freeze-dried at −38 °C, 10 Pa, for 24 h and put on a circular specimen stub and attached. The surface was sprayed with gold, and the samples were observed using a Quanta FEG 250 SEM (FEI Co., Hillsboro, OR, USA). The external microstructure of flat rice noodles was observed 2000 times. The detector was ETD. All images were obtained at the accelerating voltage of 15 kV.

Cao Z., Liu Y., Zhu H., Li Y., Xiao Q, & Yi C. (2021). Effect of Soy Protein Isolate on Textural Properties, Cooking Properties and Flavor of Whole-Grain Flat Rice Noodles. Foods, 10(5), 1085.

+ Open protocol

+ Expand

Multimodal Imaging of Porous Transport Layers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Porous transport layer (PTL) surface morphology was imaged by an FEI Quanta FEG 250 SEM, Figure S2 in the Supporting Information. Ex situ XCT was conducted at the Advanced Light Source at Lawrence Berkeley National Laboratory (Beamline 8.3.2) with a PCO.Edge CCD camera, an LuAG scintillator, and 20x optical lenses. The resulting images had a resolution of 0.323 µm voxel⁻¹ and a horizontal field of view of 1.8 mm. The samples were prepared for tomography imaging by cutting the PTLs into ≈3 mm triangular sections each having an ≈45° tip and 5 mm base for mounting on the pins which were mounted on the beamline rotating stage. A multilayer monochromator was used to select the X‐ray energy at 20 keV. 300 ms exposure time was used. Operando XCT and radiography imaging were conducted at Beamline 2‐BM at Advanced Photon Source (APS). A multilayer monochromator was used to select 27 keV energy. The optics used were Optique 2X lens, 20 um LuAGb scintillator, resulting in 1.73 um voxel⁻¹ resolution. 20 ms exposure time was used for XCT data acquisition. Radiography imaging was used to capture oxygen transport in the channels. The data were collected with the cell in‐plane and through‐plane configuration, using 5 ms exposure time for a total time of 2 min per each operating condition.

Peng X., Satjaritanun P., Taie Z., Wiles L., Keane A., Capuano C., Zenyuk I.V, & Danilovic N. (2021). Insights into Interfacial and Bulk Transport Phenomena Affecting Proton Exchange Membrane Water Electrolyzer Performance at Ultra‐Low Iridium Loadings. Advanced Science, 8(21), 2102950.

+ Open protocol

+ Expand

Electrochemical and Imaging Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols

702 titroprocessor (Metrohm, Switzerland) was used for potentiometric and pH-measurements. FESEM images were taken by (FEI- Quanta feg-250 SEM, Switzerland) for the sensor paste. HRTEM images were recorded by (Jeol 2100 HRTEM, Japan). Electrochemical impedance spectroscopy (EIS) studies were performed using potentiostat (Autolab PGSTAT 302 N, Netherlands).

Khalil M.M., Farghali A.A., El Rouby W.M, & Abd-Elgawad I.H. (2020). Preparation and characterization of novel MWCNTs/Fe-Co doped TNTs nanocomposite for potentiometric determination of sulpiride in real water samples. Scientific Reports, 10, 8607.

+ Open protocol

+ Expand

Powder Morphology Analysis by SEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

The powder morphology was observed by scanning electron microscopy (SEM). In order to assess the structural and morphological characteristics, each powder was placed on a sample holder and fixed with double-sided tape. Then, it was sputter-coated with gold and observed in a Quanta FEG 250 SEM (FEI, United States of America).

Ilie G.I., Milea Ș.A., Râpeanu G., Cîrciumaru A, & Stănciuc N. (2022). Sustainable Design of Innovative Kiwi Byproducts-Based Ingredients Containing Probiotics. Foods, 11(15), 2334.

+ Open protocol

+ Expand

Bacterial Biofilm Visualization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The bacterial cultures were grown in 5 mL TSB at 37 °C overnight and then were diluted 1:100 with TSB in 24-well plates (Beyotime, China). After incubation at 37 °C for 24 h without shaking, the catheter fragments were washed for 5 min with PBS three times. The strains were then fixed for 20 min with 4% paraformaldehyde (Sigma), followed by a secondary immobilization with 2.5% glutaraldehyde (Sigma). After overnight incubation, samples were washed three times with phosphate buffers and dehydrated in 30%, 50%, 70%, 80%, 90%, 95%, or 100% ethanol (Sigma) for at least 10 min each. Eventually, the samples were dried, ion-sputter gilded, and then captured using an FEI Quanta FEG 250 SEM with a magnification of x5000 and x20000 times [34] (link).

Zhou J., Refat M., Guo Y., Zhang J., Jiao M., He W., He X., Rabie M.A., Ouyang Z, & Zheng F. (2024). The Functional Study of Response Regulator ArlR Mutants in Staphylococcus Aureus. Applied biochemistry and biotechnology.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!