Calf thymus (CT) DNA was purchased from Rockland Immunochemicals (Pottstown, PA, USA). Deoxyadenosine monophosphate (dAMP), thymidine monophosphate (TMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP), adenosine monophosphate (AMP), uridine monophosphate (UMP), cytidine monophosphate (CMP), guanosine monophosphate (GMP), nuclease P1 and deoxyribonuclease I (DNase I) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Inosine monophosphate (IMP) was purchased from Cayman Chemical (Ann Arbor, MI, USA). DNeasy Blood & Tissue Kit was purchased from Qiagen (Valencia, CA, USA). Acetonitrile and water (MS grade) were purchased from EMD (Gibbstown, NJ, USA).
Uridine monophosphate
Manufactured by Merck Group
Sourced in United States
Uridine monophosphate is a nucleotide that serves as a building block for ribonucleic acid (RNA). It is a naturally occurring compound found in various biological systems. Uridine monophosphate is used as a laboratory reagent for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Cytidine monophosphate, by Merck Group (3 mentions)
Adenosine monophosphate, by Merck Group (3 mentions)
Guanosine monophosphate, by Merck Group (3 mentions)
Deoxyguanosine monophosphate, by Merck Group (1 mentions)
Deoxyribonuclease 1 dnase 1, by Merck Group (1 mentions)
Nuclease p1, by Merck Group (1 mentions)
Dneasy blood tissue kit, by Qiagen (1 mentions)
Adenosine, by Merck Group (1 mentions)
Sodium triphosphate pentabasic, by Merck Group (1 mentions)
Guanosine diphosphate, by Merck Group (1 mentions)
Guanosine triphosphate, by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by GoldBio (1 mentions)
Adocbl, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine tcep, by GoldBio (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Coenzyme a, by Merck Group (1 mentions)
Uracil, by Merck Group (1 mentions)
Asparagine, by Merck Group (1 mentions)
Serine, by Merck Group (1 mentions)
5 protocols using uridine monophosphate
1
Enzymatic DNA Nucleotide Analysis
2
Screening for Small Molecule Inhibitors
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Structural data for the compounds available in the chemical library were provided by Namiki Shoji (Tokyo, Japan). Compounds selected by in silico SCR were purchased from Namiki Shoji. For the control compounds, adenosine monophosphate (AMP), uridine monophosphate (UMP), cytidine monophosphate (CMP), guanine monophosphate (GMP), and adenosine were purchased from Sigma-Aldrich (St. Louis, MO, USA). BCI-137 was purchased from Enamine (Kyiv, Ukraine).
3
Purification and Analysis of Enzymes
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adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), Coenzyme A (CoA), guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GTP), uridine monophosphate (UMP), β,γ-Methyleneguanosine 5′-triphosphate (GMPPCP), sodium triphosphate pentabasic (PPPi), and AdoCbl were from Sigma-Aldrich. Isopropyl β-D-1-thiogalactopyranoside (IPTG) and Tris (2-carboxyethyl) phosphine (TCEP) were from gold biotechnology. Ni(II)-NTA resin was from Qiagen.
4
Egg Product Metabolome Analysis
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Methanol, acetonitrile, formic acid (FA), and ammonium formate (AF) were purchased from VWR International, Ltd (Poole, UK).
Analytical standards of chloramphenicol, lactic acid, succinic acid, phenyllactic acid, tyramine, uracil, asparagine, glutamine, guanosine, guanosine monophosphate, serine, uridine, and uridine monophosphate were purchased from Sigma‐Aldrich (St Louis, Missouri).
Water was purified using a Milli‐Q system (Millipore, Bedford, Massachusetts).
Egg products samples were directly collected from batches that daily arrive at production plant sites; to increase the variability of the model, the sampling period lasted 6 weeks, and samples coming from different batches, different suppliers, and with different amounts of carotenoids (in the range of tenths of milligrams per kilogram, authorized for improving appearance and correspondent consumer preference in finished products) were selected.
Analytical standards of chloramphenicol, lactic acid, succinic acid, phenyllactic acid, tyramine, uracil, asparagine, glutamine, guanosine, guanosine monophosphate, serine, uridine, and uridine monophosphate were purchased from Sigma‐Aldrich (St Louis, Missouri).
Water was purified using a Milli‐Q system (Millipore, Bedford, Massachusetts).
Egg products samples were directly collected from batches that daily arrive at production plant sites; to increase the variability of the model, the sampling period lasted 6 weeks, and samples coming from different batches, different suppliers, and with different amounts of carotenoids (in the range of tenths of milligrams per kilogram, authorized for improving appearance and correspondent consumer preference in finished products) were selected.
5
Quantification of NNK and Metabolites in Zebrafish
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4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was obtained from LGC-Dr Ehrenstorfer (LGC Standards, Barcelona, Spain) and 4-Hydroxy-4-(3-pyridyl)-butyric acid (HPBA) was purchased from Toronto Research Chemicals (TRC, Ontario, Canada). The metabolites hypoxanthine, cytidine monophosphate (CMP), guanosine monophosphate (GMP), S-adenosylmethionine (SAM), and uridine monophosphate (UMP) were obtained from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Internal standard (IS) solution was prepared with 1 mg mL -1 of succinic-d4 acid and myristic-d27 acid in methanol also from Sigma-Aldrich. All solvents used in the preparation of solutions were LC-MS grade. Fish water was prepared with reverse-osmosis purified water containing 90 µg mL -1 of Instant Ocean ® , 0.58 mM CaSO4•2H2O (Aquarium Systems, Sarrebourg, France).
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