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Peroxidase conjugated human fc specific igg

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Peroxidase-conjugated human Fc-specific IgG is a laboratory reagent that consists of an IgG antibody specific to the Fc region of human immunoglobulins, conjugated to the enzyme peroxidase. This conjugate is used as a detection tool in various immunoassay techniques.

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Lab products found in correlation

1 naphthol, by Merck Group (2 mentions) 1 8 dhn, by Merck Group (2 mentions) 1 2 dhn, by Merck Group (2 mentions) 1 4 dhn, by Merck Group (2 mentions) H2o2 detection system, by Merck Group (2 mentions) Naphthalene, by Merck Group (2 mentions)

Spelling variants of this product

IgG-Fc Specific-Peroxidase (4 citations) Horseradish peroxidase conjugated goat anti-human Fc specific polyclonal IgG (2 citations)

2 protocols using peroxidase conjugated human fc specific igg

1

Characterization of Fungal Cell Wall Interactions

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Alkali-insoluble and soluble fungal cell wall fractions were obtained as described previously34 (link). For the ELISA, wells were coated overnight with 20-200 µg/mL of the cell wall fractions, melanin ghosts (in house generated), purified YWA1 (in house generated), 1,8-DHN, 1,2-DHN, 1,4-DHN, naphthalene, 1-naphthol (all from Sigma) in 50 mM carbonate buffer pH 9.6, and then blocked with 1% BSA in PBS. Fc-MelLec (5 µg/mL) was added to the wells and incubated for 1 h at room temperature. Following washing with PBS-Tween-20 (0.5% (v/v)), peroxidase-conjugated human Fc-specific IgG (Sigma) was added to the wells and incubated for 1 h at room temperature. Following further washing, quantification of Fc-MelLec binding was detected using ortho-phenylenediamine (OPD, Sigma) and H2O2 detection system (Merck). The reaction was stopped using 4% (v/v) H2SO4 and optical densities measured at 492 nm.
Stappers M.H., Clark A.E., Aimanianda V., Bidula S., Reid D.M., Asamaphan P., Hardison S.E., Dambuza I.M., Valsecchi I., Kerscher B., Plato A., Wallace C.A., Yuecel R., Hebecker B., da Glória Teixeira Sousa M., Cunha C., Liu Y., Feizi T., Brakhage A.A., Kwon-Chung K.J., Gow N.A., Zanda M., Piras M., Zanato C., Jaeger M., Netea M.G., van de Veerdonk F.L., Lacerda J.F., Campos A J.r., Carvalho A., Willment J.A., Latgé J.P, & Brown G.D. (2018). Recognition of DHN-melanin by MelLec, is required for protective immunity to Aspergillus. Nature, 555(7696), 382-386.
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2

Characterization of Fungal Cell Wall Interactions

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Alkali-insoluble and soluble fungal cell wall fractions were obtained as described previously34 (link). For the ELISA, wells were coated overnight with 20-200 µg/mL of the cell wall fractions, melanin ghosts (in house generated), purified YWA1 (in house generated), 1,8-DHN, 1,2-DHN, 1,4-DHN, naphthalene, 1-naphthol (all from Sigma) in 50 mM carbonate buffer pH 9.6, and then blocked with 1% BSA in PBS. Fc-MelLec (5 µg/mL) was added to the wells and incubated for 1 h at room temperature. Following washing with PBS-Tween-20 (0.5% (v/v)), peroxidase-conjugated human Fc-specific IgG (Sigma) was added to the wells and incubated for 1 h at room temperature. Following further washing, quantification of Fc-MelLec binding was detected using ortho-phenylenediamine (OPD, Sigma) and H2O2 detection system (Merck). The reaction was stopped using 4% (v/v) H2SO4 and optical densities measured at 492 nm.
Stappers M.H., Clark A.E., Aimanianda V., Bidula S., Reid D.M., Asamaphan P., Hardison S.E., Dambuza I.M., Valsecchi I., Kerscher B., Plato A., Wallace C.A., Yuecel R., Hebecker B., da Glória Teixeira Sousa M., Cunha C., Liu Y., Feizi T., Brakhage A.A., Kwon-Chung K.J., Gow N.A., Zanda M., Piras M., Zanato C., Jaeger M., Netea M.G., van de Veerdonk F.L., Lacerda J.F., Campos A J.r., Carvalho A., Willment J.A., Latgé J.P, & Brown G.D. (2018). Recognition of DHN-melanin by MelLec, is required for protective immunity to Aspergillus. Nature, 555(7696), 382-386.
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