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Mabf2158

Manufactured by Merck Group
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MABF2158 is a specialized laboratory equipment manufactured by Merck Group. It is designed for specific scientific applications, but a detailed description of its core function cannot be provided in a concise, unbiased, and factual manner without the risk of extrapolation or interpretation. Therefore, a detailed description is not available.

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Lab products found in correlation

Ab228023, by Abcam (2 mentions) Ab15348, by Abcam (2 mentions) Ab15348, by Merck Group (1 mentions)

3 protocols using mabf2158

1

Immunohistochemistry of ACE2, EPCAM and TMPRSS2

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Specimens were obtained via clinical endoscopy during routine care (Table S1, S2). Tissue was formalin fixed and paraffin embedded by the clinical pathology core at our institution. Primary antibodies used included ACE2 (abcam-ab15348, 1:1000), EPCAM (abcam-ab228023, prediluted) and mouse anti-TMPRSS2 (Millipore-MABF2158, 1:500) and staining was performed as detailed in supplementary methods.
Suárez-Fariñas M., Tokuyama M., Wei G., Huang R., Livanos A., Jha D., Levescot A., Irizar H., Kosoy R., Cording S., Wang W., Losic B., Ungaro R.C., Di’Narzo A., Martinez-Delgado G., Suprun M., Corley M.J., Stojmirovic A., Houten S.M., Peters L., Curran M., Brodmerkel C., Perrigoue J., Friedman J.R., Hao K., Schadt E.E., Zhu J., Ko H.M., Cho J., Dubinsky M.C., Sands B.E., Ndhlovu L., Cerf-Bensusan N., Kasarskis A., Colombel J.F., Harpaz N., Argmann C, & Mehandru S. (2020). Intestinal Inflammation Modulates the Expression of ACE2 and TMPRSS2 and Potentially Overlaps With the Pathogenesis of SARS-CoV-2–related Disease. Gastroenterology, 160(1), 287-301.e20.
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2

SARS-CoV-2 Receptor Protein Localization

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Specimens were obtained via clinical endoscopy during routine care (Supplementary Tables 1 and 2). Tissue was formalin fixed and paraffin embedded by the clinical pathology core at our institution. Primary antibodies used included ACE2 (abcam-ab15348, 1:1000), EPCAM (abcam-ab228023, prediluted), and mouse anti-TMPRSS2 (Millipore-MABF2158, 1:500) and staining was performed as detailed in supplementary methods.
Suárez-Fariñas M., Tokuyama M., Wei G., Huang R., Livanos A., Jha D., Levescot A., Irizar H., Kosoy R., Cording S., Wang W., Losic B., Ungaro R.C., Di’Narzo A., Martinez-Delgado G., Suprun M., Corley M.J., Stojmirovic A., Houten S.M., Peters L., Curran M., Brodmerkel C., Perrigoue J., Friedman J.R., Hao K., Schadt E.E., Zhu J., Ko H.M., Cho J., Dubinsky M.C., Sands B.E., Ndhlovu L., Cerf-Bensusan N., Kasarskis A., Colombel J.F., Harpaz N., Argmann C, & Mehandru S. (2020). Intestinal Inflammation Modulates the Expression of ACE2 and TMPRSS2 and Potentially Overlaps With the Pathogenesis of SARS-CoV-2–related Disease. Gastroenterology, 160(1), 287-301.e20.
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3

Peptide Blocking of Antibody Binding

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Using peptide sequences generously provided by the manufacturers of anti-ACE2 (Abcam ab15348) and anti-TMPRSS2 (Millipore MABF2158) antibodies, we custom synthesized the immunizing ACE2 and TMPRSS2 peptides, respectively, through GenScript. The sequence of the synthesized immunizing ACE2 peptide is: KGENNPGFQNTDDVQTSF. The sequence of the synthesized immunizing TMPRSS2 peptide was provided in confidence by the manufacturer. Prior to primary antibody staining, the primary antibody was mixed and incubated with a 20-fold molar excess of immunizing peptide or an unrelated mock peptide for 3 h at room temperature. Other staining procedures were performed as described above.
Nakayama T., Lee I.T., Jiang S., Matter M.S., Yan C.H., Overdevest J.B., Wu C.T., Goltsev Y., Shih L.C., Liao C.K., Zhu B., Bai Y., Lidsky P., Xiao Y., Zarabanda D., Yang A., Easwaran M., Schürch C.M., Chu P., Chen H., Stalder A.K., McIlwain D.R., Borchard N.A., Gall P.A., Dholakia S.S., Le W., Xu L., Tai C.J., Yeh T.H., Erickson-Direnzo E., Duran J.M., Mertz K.D., Hwang P.H., Haslbauer J.D., Jackson P.K., Menter T., Andino R., Canoll P.D., DeConde A.S., Patel Z.M., Tzankov A., Nolan G.P, & Nayak J.V. (2021). Determinants of SARS-CoV-2 entry and replication in airway mucosal tissue and susceptibility in smokers. Cell Reports Medicine, 2(10), 100421.
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