Single-particle microscopy measurements were performed with beads suspended in water (milliQ) and transferred onto a coverslip. The microscopy images were recorded with a FluoView FV1000 microscope (Olympus GmbH, Germany), similar to the procedure described earlier31 (link),32 (link),36 (link). A multiline argon ion laser (488 nm, 30 mW) and a green HeNe laser (543 nm, 1 mW) were used as excitation light sources. The excitation light was reflected by a dichroic mirror DM 488/543/633 and focused onto the sample through an Olympus objective UPLSAPO 60 × W (numerical aperture N.A. 1.2). The emitted photons were collected with the same objective. The different spectral channels were defined by optical filters (detection settings: channel 1 was defined by an emission dichroic mirror SDM560 combined with a variable band pass filter position 495 nm and a filter range of 100 nm, and channel 2 by a barrier filter BA650IF).
Dm488 543 633
Manufactured by Olympus
The DM488/543/633 is a multi-laser confocal microscope system designed for fluorescence imaging. It is capable of using three different laser excitation wavelengths: 488 nm, 543 nm, and 633 nm. The system is intended for use in biological and materials science research applications.
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Lab products found in correlation
3 protocols using dm488 543 633
1
Single-Particle Microscopy Methodology
2
Live Cell IRM Imaging Protocol
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IRM of living cells immobilized on glycine-coated coverslips was recorded on a modified Olympus Fluoview 1000 setup equipped with 60x 1.2 NA water immersion objective (UPlanSApo; Olympus). GFP signal was excited with 20mW 488nm laser (Sapphire; Coherent) and recorded on a single-channel PMT detector equipped with a selective dichroic mirror (DM488/543/633; Olympus). For acquisition and system control the Fluoview 1000 software package (Olympus) was used. The images were processed using ImageJ/Fiji software package [50] .
3
Live Cell IRM Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IRM of living cells immobilized on glycine-coated coverslips was recorded on a modified Olympus Fluoview 1000 setup equipped with 60x 1.2 NA water immersion objective (UPlanSApo; Olympus). GFP signal was excited with 20mW 488nm laser (Sapphire; Coherent) and recorded on a single-channel PMT detector equipped with a selective dichroic mirror (DM488/543/633; Olympus). For acquisition and system control the Fluoview 1000 software package (Olympus) was used. The images were processed using ImageJ/Fiji software package [50] .
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