Ea hy926 human endothelial cells

b.End3 murine microvascular endothelial cells were obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK). The b.End3 cells were established form brain endothelial cells of 129/Sv mice by immortalization with the Polyoma virus middle T-antigen [29 (link)]. The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Biochrom AG, Berlin, Germany) containing 1g/l glucose supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT), 1% non-essential amino acids, 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen, Carlsbad, CA) at 37°C in 10% CO₂ atmosphere [28 (link)]. bEnd.3 microvascular cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and maintained similarly. This cell line was established by infecting BALB/c cortical capillary endothelial cells with the Polyoma middle T-antigen expressing retrovirus [30 (link), 31 (link)]. EA.hy926 human endothelial cells were obtained from ATCC (Manassas, VA) and maintained in DMEM containing 1 g/l glucose supplemented with 10% FBS, 1% non-essential amino acids, 100 IU/ml penicillin and 100 μg/ml streptomycin. HepG2 human hepatocellular carcinoma cells were obtained from ATCC (Manassas, VA) and maintained in Eagle’s minimum essential medium (EMEM, Cellgro Mediatech Inc., Manassas, VA) supplemented with 10% FBS, 100 IU/ml penicillin and 100 μg/ml streptomycin.