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Sc 32761

Manufactured by Santa Cruz Biotechnology
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Sc-32761 is a lab equipment product manufactured by Santa Cruz Biotechnology. It is a device designed to facilitate specific research and laboratory procedures. The core function of this product is to assist in the execution of various experimental protocols, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ripa buffer, by Cell Signaling Technology (2 mentions) Azure 300, by Azure Biosystems (2 mentions) Ab182561, by Cell Signaling Technology (2 mentions) Haf007, by R&D Systems (2 mentions) Sc 166944, by Santa Cruz Biotechnology (2 mentions) Haf008, by R&D Systems (2 mentions) Haf016, by R&D Systems (2 mentions) Protein a g agarose bead, by Santa Cruz Biotechnology (1 mentions) Ab110641, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail, by Santa Cruz Biotechnology (1 mentions) Dako real antibody diluent, by Agilent Technologies (1 mentions) Dako real detection system, by Agilent Technologies (1 mentions) Citra plus, by BioGenex (1 mentions)

5 protocols using sc 32761

1

Arid1a Regulation in Dental Progenitor Cells

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At PN7.5,4 days after tamoxifen induction, the apical thirds of first mandibular molars from Gli1-CreER;Arid1afl/fl mice and littermate controls were cut into pieces and homogenized in RIPA buffer (Cell Signaling, 9806s) supplemented with protease inhibitor (Thermo Fisher Scientific, A32959). Western blot was performed per standard protocol and signals were detected using Azure 300 (Azure biosystems). The primary antibodies are listed in Table S1. HRP-conjugated secondary antibodies (R&D, HAF007, HAF008, and HAF016) were used in the study. For co-immunoprecipitation (co-IP), DPCs cultured in vitro were harvested and lysed in lysis buffer [50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM EDTA (pH 8.0), 1mM PMSF, 1% NP-40, 5% glycerol]. Then lysates were subjected to immunoprecipitation with anti-Arid1a antibody (Abcam, ab182561) or normal Rabbit IgG (Cell Signaling Technology, 2729) and protein A-Sepharose (VWR, CA97067-898). Immune complexes were washed and subjected to immunoblotting with anti-Arid1a (Santa Cruz, sc-32761) or anti-Plagl1 (Santa Cruz, sc-166944) antibodies.
Du J., Jing J., Yuan Y., Feng J., Han X., Chen S., Li X., Peng W., Xu J., Ho T.V., Jiang X, & Chai Y. (2021). Arid1a-Plagl1-Hh signaling is indispensable for differentiation-associated cell cycle arrest of tooth root progenitors. Cell reports, 35(1), 108964.
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2

SMARCB1 Interacting Protein Complex

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SMARCB1 was immunoprecipitated from cell extracts using anti-SMARCB1 antibody (Sigma-Aldrich; SAB4200202) and protein A/G agarose beads (Santa Cruz Biotechnology) using 1X cell lysis buffer [20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, and protease inhibitor cocktail (Roche)]. The immunoprecipitated complex was washed using phosphate-buffered saline (PBS) (3.2 mM Na2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, pH 7.4) containing 1% Triton X-100 (PBST). Following immunoprecipitation, the samples were subjected to SDS-PAGE gel electrophoresis under denaturing conditions, and subsequently immunoblotted using antibodies against PBRM1 (rabbit polyclonal; Bethyl Laboratories, A301–591A), SMARCA4 (rabbit monoclonal; Abcam; ab110641), and ARID1A (mouse monoclonal; Santa Cruz Biotechnology; sc-32761). TrueBlot anti-rabbit or mouse IgG-HRP (Rockland) were used as secondary antibodies.
Msaouel P., Malouf G.G., Su X., Yao H., Tripathi D.N., Soeung M., Gao J., Rao P., Coarfa C., Creighton C.J., Bertocchio J.P., Kunnimalaiyaan S., Multani A.S., Blando J., He R., Shapiro D.D., Perelli L., Srinivasan S., Carbone F., Pilié P.G., Karki M., Seervai R.N., Vokshi B.H., Lopez-Terrada D., Cheng E.H., Tang X., Lu W., Wistuba I.I., Thompson T.C., Davidson I., Giuliani V., Schlacher K., Carugo A., Heffernan T.P., Sharma P., Karam J.A., Wood C.G., Walker C.L., Genovese G, & Tannir N.M. (2020). Comprehensive Molecular Characterization Identifies Distinct Genomic and Immune Hallmarks of Renal Medullary Carcinoma. Cancer cell, 37(5), 720-734.e13.
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3

ARID1A Expression in Breast Cancer Brain Metastases

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Tissue microarrays (TMA) with 132 surgical tissue specimens from histologically proven breast cancer brain metastases [7 (link)] were used for immunohistochemistry (IHC) staining of ARID1A protein. TMA slides were de-paraffinized in xylol and rehydrated by decreasing ethanol series followed by antigen unmasking by boiling in citrate buffer for 5 min at 120 °C (Citra Plus, BioGenex). Primary mouse monoclonal ARID1A antibody was diluted 1:500 (sc-32761, Santa Cruz Biotechnology) in Dako REAL Antibody Diluent (Agilent Technologies), and the TMA slide was incubated at 4 °C overnight. For visualization, Dako REAL Detection System was used (K5001, Agilent Technologies). Signal intensity (grading 0–3) and signal distribution (percentage of stained cells) were multiplied to a final value and grouped according to the final value into negative (0–0.5), intermediate (0.6–2), and strong (≥ 2) staining.
Riebensahm C., Joosse S.A., Mohme M., Hanssen A., Matschke J., Goy Y., Witzel I., Lamszus K., Kropidlowski J., Petersen C., Kolb-Kokocinski A., Sauer S., Borgmann K., Glatzel M., Müller V., Westphal M., Riethdorf S., Pantel K, & Wikman H. (2019). Clonality of circulating tumor cells in breast cancer brain metastasis patients. Breast Cancer Research : BCR, 21, 101.
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4

Arid1a Regulation in Dental Progenitor Cells

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At PN7.5,4 days after tamoxifen induction, the apical thirds of first mandibular molars from Gli1-CreER;Arid1afl/fl mice and littermate controls were cut into pieces and homogenized in RIPA buffer (Cell Signaling, 9806s) supplemented with protease inhibitor (Thermo Fisher Scientific, A32959). Western blot was performed per standard protocol and signals were detected using Azure 300 (Azure biosystems). The primary antibodies are listed in Table S1. HRP-conjugated secondary antibodies (R&D, HAF007, HAF008, and HAF016) were used in the study. For co-immunoprecipitation (co-IP), DPCs cultured in vitro were harvested and lysed in lysis buffer [50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM EDTA (pH 8.0), 1mM PMSF, 1% NP-40, 5% glycerol]. Then lysates were subjected to immunoprecipitation with anti-Arid1a antibody (Abcam, ab182561) or normal Rabbit IgG (Cell Signaling Technology, 2729) and protein A-Sepharose (VWR, CA97067-898). Immune complexes were washed and subjected to immunoblotting with anti-Arid1a (Santa Cruz, sc-32761) or anti-Plagl1 (Santa Cruz, sc-166944) antibodies.
Du J., Jing J., Yuan Y., Feng J., Han X., Chen S., Li X., Peng W., Xu J., Ho T.V., Jiang X, & Chai Y. (2021). Arid1a-Plagl1-Hh signaling is indispensable for differentiation-associated cell cycle arrest of tooth root progenitors. Cell reports, 35(1), 108964.
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5

Prognostic Significance of ARID1A in Breast Cancer

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Normalized gene expression data for 1095 primary breast cancers and 113 normal breast tissues, assayed by RNA-sequencing, was gained from the TCGA data analysis website (http://www.cbioportal.org/index.do). The intergroup difference was evaluated using a t-test. The relevance of mRNA expression of ARID family members to OS was assessed on an network database (https://kmplot.com), which was constituted from Gene Expression Omnibus(GEO) [32 (link)]. Survival curves of ARID1A protein expression were plotted using the Kaplan–Meier method, the effect of different variables on overall survival was evaluated using the Cox regression analyses. p-value<0.05 was considered to be statistically significant.(*p<0.05, **p<0.01, ***p<0.001).
The expression of ARID1A protein was performed by Immunohistochemistry staining(IHC). Each tissue section was incubated with ARID1A mouse polyclonal antibody solution (SC-32761, Santa Cruz, CA, USA; 1:200 dilution). The staining scoring was calculated by intensity*percentage of positive cells. The IHC staining intensity was graded as 0(no staining), 1 (weak staining), 2 (moderate staining), and 3 (strong staining). The positive staining tumor cells proportion in a field was calculated as 0 (<5%), 1 (5–20%), 2 (20–50%), 3 (50–75%), and 4(> 75%).
Zhang J., Hou S., You Z., Li G., Xu S., Li X., Zhang X., Lei B, & Pang D. (2021). Expression and prognostic values of ARID family members in breast cancer. Aging (Albany NY), 13(4), 5621-5637.
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