Anti sma

Manufactured by Agilent Technologies

Sourced in United Kingdom

The Anti-SMA product is a laboratory equipment used to detect and quantify the presence of Smooth Muscle Actin (SMA) in biological samples. It functions as an analytical tool to support research and diagnostic applications.

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Lab products found in correlation

Anti ssea 4, by Developmental Studies Hybridoma Bank (3 mentions) Tuj1 anti tubulin beta 3 isoform, by Merck Group (3 mentions) Anti musashi, by Merck Group (3 mentions) Anti nestin, by R&D Systems (3 mentions) Anti afp, by Agilent Technologies (3 mentions) Anti map2, by Merck Group (3 mentions) Anti nanog, by R&D Systems (3 mentions) Anti sox2, by Merck Group (3 mentions) Anti oct4, by Santa Cruz Biotechnology (3 mentions) Anti tbr1, by Abcam (2 mentions) Anti ctip2, by Abcam (2 mentions) Anti tra 1 81, by Merck Group (2 mentions) At8 anti p tau, by Thermo Fisher Scientific (2 mentions) Anti lc3b, by Cell Signaling Technology (2 mentions) Anti s100, by Agilent Technologies (1 mentions) Dab peroxidase substrate kit, by Beyotime (1 mentions) Lf200 microscope, by Leica (1 mentions) Anti calponin, by Agilent Technologies (1 mentions) Cy2 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Tomato lectin fluorescein, by Vector Laboratories (1 mentions)

7 protocols using anti sma

Immunohistochemical Staining Protocol for Cell Markers

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IHC staining was carried out as previously described [15 (link)]. In brief, tissue Sections (4 μm) were dewaxed in xylene twice for 2 min each and then rehydrated in a graded series of ethanol (100–70%). Antigen retrieval was performed by boiling sections for 15 min in sodium citrate buffer (10 mM citrate acid, 10 mM sodium citrate, pH 6.0). Then, 5% normal donkey serum was used to block nonspecific antigens. IHC was performed using the Dako EnvisionTM method for antibody incubation and then developed by using the DAB peroxidase substrate kit (Beyotime, P0202). IHC-stained sections were imaged by a Leica LF200 microscope.
Antibodies for IHC included anti-S100 (Dako Z0311, 1:200), anti-Calponin (Dako M3556, 1:100), anti-SMA (Dako M0851, 1:100), anti-CK7 (Dako M7018, 1:50) and anti-CK19 (Dako M0888, 1:100), anti-FZD2 (Bioworld BS3163, 1:50), which were purchased from commercial sources.

Han Z., Ren H., Sun J., Jin L., Wang Q., Guo C, & Tian Z. (2022). Integrated weighted gene coexpression network analysis identifies Frizzled 2 (FZD2) as a key gene in invasive malignant pleomorphic adenoma. Journal of Translational Medicine, 20, 15.

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Multicolor Immunofluorescence of Vasopressin

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The following primary and secondary antibodies were used: anti-AVP (rabbit, ImmunoStar, 20069, Hudson, WI); antitype IV collagen (goat, SouthernBiotech, 1340-01, Birmingham, AL); anti-SMA (1:67, mouse, Dako, M0851, Santa Clara, CA): donkey antirabbit Cy2 (Jackson ImmunoResearch, 711-225-152, West Grove, PA); donkey antirabbit Cy3 (Jackson ImmunoResearch, 711-165-152); donkey antimouse Cy3 (Jackson ImmunoResearch, 715-165-151); donkey antigoat Cy5 (Jackson ImmunoResearch, 705-175-147); tomato lectin-fluorescein (Vector laboratories, FL-1171, Burlingame, CA).

Yao Y., Taub A.B., LeSauter J, & Silver R. (2021). Identification of the suprachiasmatic nucleus venous portal system in the mammalian brain. Nature Communications, 12, 5643.

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Immunocytochemical and Western Blot Analysis

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Immunocytochemistry and Western blot analysis were performed as we described before.²⁷, ²⁸ The following primary antibodies were used: anti‐OCT4 (1:200; Santa Cruz), anti‐SOX2 (1:200; Millipore), anti‐NANOG (1:200; R&D Systems), anti‐SSEA‐4 (1:100; Developmental Studies Hybridoma Bank), anti‐TRA‐1‐81 (1:100; Chemicon), Tuj1 anti‐tubulin beta III isoform (1:200; Millipore), anti‐SMA (1:100; DAKO); anti‐AFP (1:100; DAKO), anti‐Nestin (1:200; R&D Systems), anti‐Musashi (1:200; Millipore), anti‐Map2 (1:200; Millipore), anti‐TBR1 (1:100; Abcam), anti‐CTIP2 (1:100; Abcam), Aβ₄₂ anti‐β‐Amyloid₄₂ (1:500; Calbiochem), AT8 anti‐p‐Tau (1:1000; Thermo Fisher Scientific) and anti‐LC3B (1:500; Cell Signaling), Tau5 anti‐tau (1:1000; Thermo Fisher Scientific), anti‐Mfn1 (1:1000; Abcam), anti‐Mfn2 (1:1000; Cell Signaling), anti‐DRP1 (1:1000; Cell Signaling), anti‐Fis1 (1:1000; Santa Cruz), anti‐Ub (1:4000; Santa Cruz), anti‐LAMP2 (1:1000; Santa Cruz), anti‐Beclin1 (1:1000; Cell Signaling), p62 anti‐SQSTM1 (1:1000; Santa Cruz) and anti‐β‐actin (1:10 000; Santa Cruz).

Li L., Kim H.J., Roh J.H., Kim M., Koh W., Kim Y., Heo H., Chung J., Nakanishi M., Yoon T., Hong C.P., Seo S.W., Na D.L, & Song J. (2020). Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F). Cell Proliferation, 53(4), e12798.

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Double Immunohistochemistry for Vascular and Renal Analysis

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Double immunohistochemical staining with mouse anti-CD34 (Dako, Glostrup, Denmark) and anti-SMA (Dako) was performed to identify endothelial cells and smooth muscle cells of the arterial media (Figure 1A). The PAS stain was then applied to the sections to identify the tubular basement membranes, Bowman’s capsules, sclerotic glomeruli, and interstitial fibrosis (Figure 2F,G, Figure 3A,G, Figure 4F,G, Figure 5C,F,G and Figure 6G). Details of the tissue preparation are described elsewhere [7 (link),8 (link),9 (link),10 (link)]. Each stained specimen was digitized using virtual slide microscopy (NanoZoomer RS™; Hamamatsu Photonics, Hamamatsu, Japan), using a 40× optical lens.

Uesugi N., Shimazu Y., Kikuchi K, & Nagata M. (2016). Age-Related Renal Microvascular Changes: Evaluation by Three-Dimensional Digital Imaging of the Human Renal Microcirculation Using Virtual Microscopy. International Journal of Molecular Sciences, 17(11), 1831.

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Immunofluorescence Assay for Pluripotency and Neural Markers

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The cells were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature (RT). Fixed cells were permeabilized and washed with TPBS containing 0.1% TritonX-100 (Sigma) in phosphate-buffered saline (PBS) and blocked with 5% normal horse serum (Vector Labs) for 30 min at RT. Afterwards, they were incubated with primary antibodies in blocking solution overnight with gentle rocking at 4℃. After washing three times with TPBS, they were incubated with secondary antibodies (ThermoFisher) for 90 min at RT, and then were counterstained using DAPI for 15 min. All fluorescence images were captured by confocal microscopy (TCSSP5II, Leica). The following primary antibodies were used: anti-OCT4 (1:200, Santa Cruz), anti-SOX2 (1:200, Millipore), anti-NANOG (1:200, R&D Systems), anti-SSEA-4 (1:100, Developmental Studies Hybridoma Bank)), anti-TRA-1-81 (1:100, CHEMICON), Tuj1 anti-tubulin beta III isoform (1:200, Millipore), anti-SMA (1:100, DAKO); anti-AFP (1:100, DAKO), anti-Nestin (1:200, R&D Systems), anti-Musashi (1:200, Millipore), anti-Map2 (1:200, Millipore), anti-TBR1 (1:100, Abcam), anti-CTIP2 (1:100, Abcam), 6E10 anti-Amyloid β (1:500, Covance), AT8 anti-p-tau (1:1000, ThermoFisher), anti-ChAT (1:200, Millipore) and anti-LC3B (1:500, Cell Signaling).

Li L., Roh J.H., Chang E.H., Lee Y., Lee S., Kim M., Koh W., Chang J.W., Kim H.J., Nakanishi M., Barker R.A., Na D.L, & Song J. (2018). iPSC Modeling of Presenilin1 Mutation in Alzheimer's Disease with Cerebellar Ataxia. Experimental Neurobiology, 27(5), 350-364.

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Immunofluorescence Staining of FIZZ1 and SMA

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Immunofluorescence staining was carried out as described previously (37 (link)). Briefly, the paraffin sections were blocked with appropriate blocking serum (Vector Laboratories, Burlingame, CA) for 1 hour at room temperature and then treated with anti-FIZZ1 (Abcam, Cambridge, UK) and anti-SMA (Dako) antibodies. Then the sections were incubated with the appropriate fluorochrome-coupled secondary antibodies (Jackson ImmunoResearch, West Grove, PA). Finally, the sections were washed in PBS, mounted with ProLong® Gold antifade reagent with DAPI (Invitrogen, Grand Island, NY), and covered and sealed with a glass coverslip. Negative control sections for the immunohistochemical experiments received identical treatments but were not exposed to the primary antibody; they showed no specific staining. Staining was imaged with a Zeiss 510 Meta confocal microscope (Carl Zeiss Microscopy, Thornwood, NY) at the Johns Hopkins School of Medicine Microscope Facility.

Dasgupta P., Dorsey N.J., Li J., Qi X., Smith E.P., Yamaji-Kegan K, & Keegan A.D. (2016). Insulin Receptor Substrate (IRS)-2 negatively regulates alternative macrophage activation and allergic lung inflammation. Science signaling, 9(433), ra63.

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Immunostaining Protocols for Stem Cell Characterization

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All neurons were prepared by the methods described in a previous report [23 (link)]. The following primary antibodies were used: anti-OCT4 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-SOX2 (1:200, Millipore, Burlington, MA, USA), anti-NANOG (1:200, R&D Systems), anti-SSEA-4 (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA, USA), anti-TRA-1-81 (1:100, Thermo Fisher Scientific), Tuj1 anti-tubulin beta III isoform (1:200, Millipore), Tuj1 anti-tubulin beta III (1:200, Abcam, Cambridge, UK), anti-SMA (1:100, DAKO, Santa Clara, CA, USA), anti-AFP (1:100, DAKO), anti-Nestin (1:200, R&D Systems), anti-Musashi (1:200, Millipore), anti-MAP2 (1:200,Millipore), anti-TDP43 (1:200, Proteintech, Rosemont, IL, USA), anti-FUS/TLS (1:200, Santa Cruz Biotechnology), p-Tau (AT8), and anti-Caspase-3 (1:100, Millipore).

Kim M., Kim H.J., Koh W., Li L., Heo H., Cho H., Lyoo C.H., Seo S.W., Kim E.J., Nakanishi M., Na D.L, & Song J. (2020). Modeling of Frontotemporal Dementia Using iPSC Technology. International Journal of Molecular Sciences, 21(15), 5319.

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