Il 13 elisa kit

Recombinant dTCTP protein prepared as previously described (Kim et al., 2009 (link)), was used to screen for substances effective in the allergic airway inflammation in vitro. Cardamonin was purchased from Sigma Aldrich (St. Louis, MO, United States) and helichrysetin was purchased from ChemFaces (Hubei, China). Alum and ovalbumin (OVA) were purchased from Thermofischer scientific (Waltham, MA, United States). IL-4, IL-8, and OVA-specific IgE ELISA kit was purchased from Biolegend (San Diego, CA, United States). IL-13 ELISA kit was purchased from R&D systems (Minneapolis, MN, United States).

Imject^TM Alum Adjuvant was obtained from Pierce Biotechnology (Rockford, IL, USA). MitoSOX^TM Red was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Mouse IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and IgE ELISA kits were purchased from BD Biosciences (San Diego, CA, USA). IL-13 ELISA kit was obtained from R&D Systems (Minneapolis, MN, USA). Specific primary antibodies for signal transducer and activator of transcription 6 (STAT6), p21, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibody for phospho-STAT6 was obtained from Abcam (Cambridge, UK). Antibodies for phospho-STAT1, STAT1, phospho-STAT6, STAT6, phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK, JNK, NRF2, and HO-1 were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-CD3 and anti-CD28 antibodies were purchased from Biolegend (San Diego, CA, USA). SP600125 was purchased from Calbiochem (San Diego, CA, USA). U0126 was obtained from Promega (Madison, WI, USA) and 2′,7′-Dichlorofluorescein-diacetate (DCFH-DA) was purchased from Invitrogen (Carlsbad, CA, USA). Propidium iodide (PI), SB203580, TMA, ovalbumin (OVA) (Grade VI), dexamethasone (Dex), concanavalin A (Con A), N-acetylcysteine (NAC), and MQL were purchased from Sigma-Aldrich (St. Louis, MO, USA).