Tannin (gallotannin) was purchased from LOBA Chemie (Mumbai, India), and methyl gallate was purchased from Sigma (Steinheim, Germany). Ingredients such as peptone, yeast extract, malt extract, and carbon sources such as glucose, galactose, sucrose, maltose, lactose, raffinose, and trehalose were purchased from HiMedia (Nashik, India). The genomic DNA of yeast was extracted using a Wizard® Genomic DNA purification kit (Promega Corp., Madison, WI, United States). Nucleic acid amplifications were performed using TaKaRa Ex Taq® (Shiga, Japan). Other reagents and solvents were of analytical grade.
Raffinose
Manufactured by HiMedia
Sourced in India
Raffinose is a trisaccharide found in various plant-based sources. It functions as a carbohydrate and can be utilized in laboratory settings for various applications.
Automatically generated - may contain errors
Lab products found in correlation
Galactose, by HiMedia (1 mentions)
Maltose, by HiMedia (1 mentions)
Trehalose, by HiMedia (1 mentions)
Methyl gallate, by Merck Group (1 mentions)
Wizard genomic dna purification kit, by Promega (1 mentions)
Peptone, by HiMedia (1 mentions)
Sucrose, by HiMedia (1 mentions)
Lactose, by HiMedia (1 mentions)
Yeast extract, by HiMedia (1 mentions)
Malt extract, by HiMedia (1 mentions)
Phenol red broth base, by HiMedia (1 mentions)
Sorbitol, by HiMedia (1 mentions)
2 protocols using raffinose
1
Yeast Growth on Diverse Carbohydrates
2
Biochemical Biotyping of Clinical Isolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biochemical tests were performed to determine the biotypes of the clinical isolates as described by Shklair and Keene[22 (link)] and Yoo et al.[19 (link)] A phenol red broth base (HiMedia, India) was used as the basal medium for fermentation of mannitol, melibiose, sorbitol and raffinose (HiMedia, India). Arginine dihydrolase broth (HiMedia, India) was also employed in biochemical characterization. The test organisms were inoculated into the sterile broth and anaerobically incubated at 37°C for 48 h. The positive result was indicated by a color change as described by the manufacturer. The biochemical tests were confirmed with reference strains and repeated thrice to confirm the reproducibility and reliability.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!