20 plan apochromat dry na

For immunofluorescence analyses, images were collected at room temperature on an AxioImagerZ.1 upright microscope (Zeiss) equipped with a Zeiss 10× Plan-Neofluar dry NA 0.3 and/or Zeiss 20× Plan-Apochromat dry NA 0.8 and/or Zeiss 20× Plan-Neofluar dry NA 0.5, a DAPI filter (excitation [Ex] 365/12 nm, dichroic mirror [DM] 395 nm, emission [Em] 445/50), a GFP filter (Ex 470/40 nm, DM 495 nm, and Em 525/50 nm), a rhodamine filter (Ex 546/12 nm, DM 560 nm, and Em 608/65 nm), a Texas red filter (Ex 560/40 nm, DM 582 nm, and Em 630/785 nm), and/or a Cy5 filter (Ex 640/30 nm, DM 660 nm, Em 690/50 nm). For mosaic acquisitions, the microscope was equipped with an automated xy stage (Märzhäuser). Images were acquired with a monochrome MRm axiocam camera (Zeiss) controlled with Axiovision 4.9.1 software (Zeiss). Posttreatment image acquisition and analysis were performed using a homemade macro on Fiji (Schindelin et al., 2012 (link)). For histological analyses, images were collected at room temperature on an Axioplan2 upright microscope (Zeiss) equipped with a Zeiss 10× Plan-Neofluar PH1 dry NA 0.3 objective lens. Images were acquired with a color Infinity3-6 camera (Lumenera Corporation) controlled with Micro-Manager 1.48 software (Edelstein et al., 2014 (link)).