Dipeptidyl peptidase 4

Manufactured by Merck Group

Sourced in United States

Dipeptidyl Peptidase IV is a laboratory enzyme used for the analysis of biological samples. It functions as a protease, cleaving specific peptide bonds to break down proteins into smaller fragments. This product is intended for research use only and its performance characteristics have not been established for clinical diagnostic applications.

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Lab products found in correlation

Close spelling variants of this product

Dipeptidyl peptidase-4 (DPP-4) inhibitor Dipeptidyl peptidase 4 inhibitor Dipeptidyl Peptidase IV (DPP-IV, Peptidase

4 protocols using dipeptidyl peptidase 4

Chickpea Enzyme Digestion and Characterization

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Kabuli chickpeas were provided by the Hebrew University of Jerusalem, Israel. Pepsin (MEROPS ID: A01.071), pancreatin and stem bromelain (EC 3.4.22.32) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The chromogenic substrate Gly-Pro-pNA and dipeptidyl peptidase IV (EC 3.4.14.5), isolated from porcine kidney, were obtained from Sigma–Aldrich (St. Louis, MO, USA). α-amylase (EC 3.2.1.1), isolated from porcine pancreas, was also obtained from Sigma-Aldrich (St. Louis, MO, USA).

Chandrasekaran S., Luna-Vital D, & de Mejia E.G. (2020). Identification and Comparison of Peptides from Chickpea Protein Hydrolysates Using Either Bromelain or Gastrointestinal Enzymes and Their Relationship with Markers of Type 2 Diabetes and Bitterness. Nutrients, 12(12), 3843.

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Peptidase Activity Assay Protocol

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Porcine pepsin (EC 3.4.23.1, from porcine gastric mucosa, 3850 U mg⁻¹ protein), pancreatin from porcine pancreas (4 × USP specifications (5.46 U mg⁻¹ based on trypsin activity), EC 232-468-9), Dipeptidyl Peptidase IV (DPP-IV from porcine kidney, EC 3.4.14.5, ≥10 U mg⁻¹ protein), Gly-Pro-p-nitroanilide hydrochloride, Angiotensin Converting Enzyme (ACE from rabbit lung, EC 3.4.15.1), and all other reagents were purchased from Sigma-Aldrich (Sigma-Aldrich, Steinheim, Germany). The Active Glucagon-Like Peptide RIA kit (Cat.# GLP1A-35HK) was purchased from Merck Millipore (Merck-Millipore, Darmstadt, Germany). The Gastrin/CCK kit was purchased from CisBio (CisBio, Saclay, France). Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C) was purchased from R&D Systems (R&D Sytems, Minneapolis, MN, USA).

Dugardin C., Cudennec B., Tourret M., Caron J., Guérin-Deremaux L., Behra-Miellet J., Lefranc-Millot C, & Ravallec R. (2020). Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion. Nutrients, 12(12), 3746.

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Enzymatic Hydrolysis of Canary Seeds

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Hairless canary seeds of the CDC Cibo variety were purchased from a commercial vendor (Canpulse Foods LTD, Saskatoon, SK, Canada). Alcalase® (protease from Bacillus licheniformis, EC 3.4.21.62) and Lecitase® Ultra (Phospholipase A1 from Aspergillus oryzae) were purchased from Sigma Aldrich (St. Luis, MO, USA). Human Angiotensin-Converting Enzyme (ACE), Dipeptidyl Peptidase IV (DPP-IV, ≥ 4500 units/ μg protein), and their substrates Gly-Pro p-nitroanilide hydrochloride, and Hippuryl-L-Histidyl-L-Leucine (HHL), respectively, were all purchased from Sigma Aldrich (St. Louis, MO, USA). All chemicals used in this study were reagentgrade obtained by three leading companies VWR International (Radnor, PA, USA), Sigma Aldrich (St. Louis, MO, USA), and Thermo Fisher Scientific (Waltham, MA, USA).

Urbizo-Reyes U., Aguilar-Toalá J.E., & Liceaga A.M. (2021). Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides. Food Production Processing and Nutrition, 3(1).

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Enzymatic Digestion of Mare Milk

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Mare milk was sampled in a traditional pasture area, Inner Mongolia, China. Papain (EC 3.4.22.21 , from papaya latex, 0.5-2.0 units/mg), pepsin (EC 3.4.23.1, from porcine gastric mucosa, 250 units per milligram), pancreatin (from porcine pancreas, 8 × UPS), Gly-Pro-p-nitroanilide (H-Gly-Pro-pNA•HCl), dipeptidyl peptidase-IV (EC 3.4.14.5, from porcine kidney, ≥10 units per milligram), and diprotin A (Ile-Pro-Ile) were purchased from Sigma-Aldrich (St. Louis, MO). The HPLC-grade acetonitrile, trifluoroacetic acid, and formic acid were from Fisher Scientific (Fair Lawn, NJ). All other chemicals used in the study were of analytical grade.

Song J.J., Wang Q., Du M., Ji X.M, & Mao X.Y. (2017). Identification of dipeptidyl peptidase-IV inhibitory peptides from mare whey protein hydrolysates. Journal of dairy science, 100(9).

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