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Cd21 b ly4

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The CD21 (B-ly4) is a laboratory equipment product designed for the detection and analysis of CD21 expression on B cells. It serves as a tool for researchers in the fields of immunology and cell biology to study B cell function and differentiation.

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Lab products found in correlation

Cd8 sk1, by BD (3 mentions) Cd95 dx2, by BD (2 mentions) Cd4 l200, by BD (2 mentions) Facscanto 2 flow cytometer, by BD (2 mentions) Cd19 sj25c1, by BD (2 mentions) Gallios flow cytometer, by Beckman Coulter (2 mentions) Cd3 sk7, by BD (2 mentions) Cd38 hit2, by BD (2 mentions) Igd ia6 2, by BD (2 mentions) Cd62l dreg 56, by BD (2 mentions) Cd45ra hi100, by BD (2 mentions) Cd31 wm59, by Thermo Fisher Scientific (2 mentions) Cd45ro uchl1, by BD (2 mentions) Igm mhm 88, by BioLegend (2 mentions) Cd10 hi10a, by BD (2 mentions) Cd138 b b4, by Bio-Rad (2 mentions) Iga g20 359, by BD (2 mentions) γδ tcr b1, by Thermo Fisher Scientific (2 mentions) αβ tcr ip26, by Thermo Fisher Scientific (2 mentions) Cd57 nk 1, by BD (2 mentions)

Spelling variants of this product

CD21-BUV737 (B-ly4), (4 citations) CD21 Pe B-ly4 (3 citations) PE-Cy5-CD21 (B-ly4, (2 citations) CD21 APC(B-Ly4), (2 citations)

6 protocols using cd21 b ly4

1

Cytometric Profiling of PBMC Subsets

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PBMCs were labeled with a combination of the following mAbs: CD3 (SP 34-2), CD4 (L-200), CD8 (SK1), CD20 (2H7), CD21 (B-ly4), CD27 (M-T 271), CD28 (CD28.2), CD95 (DX2), and IgM (G20-127) (BD Pharmingen, San Jose, CA). CD38 (OKT10) was purchased from Nonhuman Primate Reagent Resource (Boston, MA). Phenotypic markers for memory and naive T cells in cynomolgus monkeys were chosen based on the studies by Pitcher et al (22 (link)). And also mature and immature B cells were chosen based on the studies by Liu et al (23 (link)). The fluorescence of the stained samples was analyzed using FACSverse (BD Biosciences) and Acculi flow cytometers (BD PharMingen, San Jose, CA), and FlowJo software (Tree Star, Inc., Ashland, OR).
Oura T., Hotta K., Lei J., Markmann J., Rosales I., Dehnadi A., Kawai K., Ndishabandi D., Smith R.N., Cosimi A.B, & Kawai T. (2016). Immunosuppression with CD40 Costimulatory Blockade plus Rapamycin for Simultaneous Islet and Kidney Transplantation in Nonhuman Primates. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 17(3), 646-656.
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2

Comprehensive Immunophenotyping of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated through a Ficoll step gradient and stained for cell surface markers with the following fluorochrome-conjugated antibodies: CD38 (HIT-2, BD), IgD (IA6-2, BD), IgM (MHM-88, BioLegend), CD10 (HI10a, BD), CD19 (SJ25C1, BD), CD21 (B-ly4, BD), CD27 (0323, eBiosience), CD138 (B-B4, AbD SeroTec), IgG (SouthernBioTech #2043-09), IgA (G20-359, BD), CD3 (SK7, BD), CD28 (CD28.2, BD), FOXP3 (PCH101, eBioscience), CD8 (SK1, BD), CD4 (RPA-T4, eBioscience), γδ-TCR (B1.1, eBioscience), αβ-TCR (IP26, eBioscience), CD45RO (UCHL1, BD), CD45RA (HI100, BD), CD57 (NK-1, BD), CD31 (WM59, eBioscience), CD62L (DREG-56, BD), CD152/CTLA4 (BNI3, BD). Samples were acquired on a FACS-Canto II flow cytometer (BD) or on a Gallios flow cytometer (Beckman Coulter, Miami, FL) and analyzed using FlowJo version 7.6.5 analysis software (Treestar, Ashland, OR).
Schubert D., Bode C., Kenefeck R., Hou T.Z., Wing J.B., Kennedy A., Bulashevska A., Petersen B.S., Schäffer A.A., Grüning B.A., Unger S., Frede N., Baumann U., Witte T., Schmidt R.E., Dueckers G., Niehues T., Seneviratne S., Kanariou M., Speckmann C., Ehl S., Rensing-Ehl A., Warnatz K., Rakhmanov M., Thimme R., Hasselblatt P., Emmerich F., Cathomen T., Backofen R., Fisch P., Seidl M., May A., Schmitt-Graeff A., Ikemizu S., Salzer U., Franke A., Sakaguchi S., Walker L.S., Sansom D.M, & Grimbacher B. (2014). Autosomal-dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nature medicine, 20(12), 1410-1416.
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3

Profiling SARS-CoV-2 Omicron-specific B cells

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Before staining with antibodies and RBD tetramers, B cells were enriched from PBMCs using a Pan B cell Isolation Kit (Miltenyi Biotech, #130-101-638) and LS column (#130-042-401); 1 × 106 B cells were incubated with BD Horizon™ Fixable Viability Stain 510 for 15 min at room temperature in the dark. After washing, the cells were stained at 4 °C, protected from light for 30 min, using a panel of fluorochrome-conjugated primary antibodies and RBD tetramers in the optimized concentrations. The antibodies in the panel, mouse anti-human CD3 (SK7), CD56 (NCAM16.2), CD14 (M5E2), CD19 (HIB19), CD20 (2H7), CD27 (M-T271), IgD (IA6-2), CD38 (HB-7, BioLegend), CD10 (HI10a, BioLegend), CD21 (B-ly4, BD Biosciences), CD38 (HIT2), CD138 (MI15), IgM (UCH-B1), and IgG (G18-145), were purchased from BD Biosciences. Fluorescent-conjugated Omicron RBD-APC and -PE proteins were prepared by mixing biotinylated RBD protein (Acro Biosystem, SPD-C82E4) and Streptavidin-APC or -PE in a 4:1 molar ratio and incubated for 15 min at room temperature. Flow cytometry was performed on a BD LSRII (BD Biosciences), and data were acquired with BD FACSDiva Software (8.0.2) and analyzed using FlowJo software (version 10).
Zhang B., Huo J., Huang Y., Teo S.Y., Duan K., Li Y., Toh L.K., Lam K.P, & Xu S. (2022). mRNA Booster Vaccination Enhances Antibody Responses against SARS-CoV2 Omicron Variant in Individuals Primed with mRNA or Inactivated Virus Vaccines. Vaccines, 10(7), 1057.
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4

Multiparameter Flow Cytometry of Macaque Immune Cells

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The following fluorochrome conjugated monoclonal antibodies reactive with macaque cells were used for flow cytometry studies: CD3 (SP34-2, BD Biosciences [BD]), CD4 (L200, BD), CD8 (SK1, BD), CD95 (DX2, BD), CD28 (CD28.2, BioLegend), CCR5 (3A9, BD), CXCR5 (MU5UBEE, Invitrogen), PD-1 (EH12.2H7, BioLegend), ICOS (C398.4A, BioLegend), CCR7 (150503, BD), α4β7 (A4B7, NHP Reagent Resource), LAG3 (3DS223H, eBioscience), Tim-3 (F38-2E2, BioLegend), TIGIT (MBSA43, Invitrogen), CD20 (2H7, BioLegend), CD19 (J3-119, Beckman Coulter), HLA-DR (L243, BioLegend), CD10 (HI10A, BioLegend), CD21 (B-ly4, BD), CD27 (O323, BioLegend), IgD (IADB6, Southern Biotech), IgG (G18-145, BD), IL-21R (2G1-K12, BioLegend), Ki-67 (B56, BD), BCL6 (IG191E/A8, BioLegend), CD80 (2D10, BioLegend), CD56 (B159, BD), CD45 (D058-1283, BD), CD14 (MoP9, BD), CD16 (3G8, BD), CCR2 (48607, R&D Systems), CD11b (ICRF44, BioLegend), CD11c (3.9, Invitrogen), PD-L1 (29E.2A3, BioLegend), PD-L2 (24F.10C12, BioLegend), CD80 (2D10, BioLegend), CD86 (FUN-1, BD), CD141 (1A4, BD), CD163 (GHI/61, BioLegend), and CD123 (7G3, BD).
Kvistad D., Pallikkuth S., Sirupangi T., Pahwa R., Kizhner A., Petrovas C., Villinger F, & Pahwa S. (2021). IL-21 enhances influenza vaccine responses in aged macaques with suppressed SIV infection. JCI Insight, 6(20), e150888.
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5

Comprehensive Immunophenotyping of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated through a Ficoll step gradient and stained for cell surface markers with the following fluorochrome-conjugated antibodies: CD38 (HIT-2, BD), IgD (IA6-2, BD), IgM (MHM-88, BioLegend), CD10 (HI10a, BD), CD19 (SJ25C1, BD), CD21 (B-ly4, BD), CD27 (0323, eBiosience), CD138 (B-B4, AbD SeroTec), IgG (SouthernBioTech #2043-09), IgA (G20-359, BD), CD3 (SK7, BD), CD28 (CD28.2, BD), FOXP3 (PCH101, eBioscience), CD8 (SK1, BD), CD4 (RPA-T4, eBioscience), γδ-TCR (B1.1, eBioscience), αβ-TCR (IP26, eBioscience), CD45RO (UCHL1, BD), CD45RA (HI100, BD), CD57 (NK-1, BD), CD31 (WM59, eBioscience), CD62L (DREG-56, BD), CD152/CTLA4 (BNI3, BD). Samples were acquired on a FACS-Canto II flow cytometer (BD) or on a Gallios flow cytometer (Beckman Coulter, Miami, FL) and analyzed using FlowJo version 7.6.5 analysis software (Treestar, Ashland, OR).
Schubert D., Bode C., Kenefeck R., Hou T.Z., Wing J.B., Kennedy A., Bulashevska A., Petersen B.S., Schäffer A.A., Grüning B.A., Unger S., Frede N., Baumann U., Witte T., Schmidt R.E., Dueckers G., Niehues T., Seneviratne S., Kanariou M., Speckmann C., Ehl S., Rensing-Ehl A., Warnatz K., Rakhmanov M., Thimme R., Hasselblatt P., Emmerich F., Cathomen T., Backofen R., Fisch P., Seidl M., May A., Schmitt-Graeff A., Ikemizu S., Salzer U., Franke A., Sakaguchi S., Walker L.S., Sansom D.M, & Grimbacher B. (2014). Autosomal-dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nature medicine, 20(12), 1410-1416.
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6

Multiparameter Flow Cytometry Profiling of PBMCs

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PBMCs from HIV-infected or HIV-negative patients were incubated with fluorochrome-conjugated antibodies for at least 15–20 minutes at 4°C or on ice, protected from light. The following fluorochrome-conjugated anti-human antibodies were used: CD3 (HIT3α), CD4 (RPA-T4), CD10 (HI10a), CD20 (2H7), CD25 (BC96), CD27 (O323), CD38 (HIT2), PD-1 (EH12.2H7), and CXCR3 (G025H7) were all from BioLegend. CD19 (HIB19), CXCR5 (RF8 B2), ICOS (DX29) and CD21 (B-ly4) were from BD Biosciences and CD45RA (2H4LDH11LDB9) from Beckman Coulter. LIVE/DEAD Fixable Dead Cell Stain (Life Technologies) was used to gate on live cells; and in some cases both LIVE/DEAD stain and Annexin V (BD Biosciences) were used. Samples were acquired on a BD LSR II.
Muir R., Metcalf T., Tardif V., Takata H., Phanuphak N., Kroon E., Colby D.J., Trichavaroj R., Valcour V., Robb M.L., Michael N.L., Ananworanich J., Trautmann L, & Haddad E.K. (2016). Altered Memory Circulating T Follicular Helper-B Cell Interaction in Early Acute HIV Infection. PLoS Pathogens, 12(7), e1005777.
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