Dansyl chloride dns cl

All reagents and solvents were of analytical grade and were used without purification. Tetrachloroauric acid (HAuCl₄), sodium citrate dihydrate (C₆H₅Na₃O₇ $·$ 2H₂O), ethanol (C₂H₅OH), aqueous ammonia (NH_3·H₂O; 25%), tetraethyl orthosilicate (SiC₈H₂₀O₄, TEOS), hydrazine monohydrate (N₂H₄ $·$ H₂O; 40%), hexadecyltrimethylammonium bromide (C₁₉H₄₂BrN, CTAB), toluene (C₇H₈), (aminopropyl)trimethoxysilane (H₂N(CH₂)₃Si(OCH₃)₃, APTMS), Fmoc–glycine (C₁₇H₁₅NO₄, Fmoc–Gly–OH), N,N′-diisopropylcarbodiimide (C₇H₁₄N₂, DIC), N,N-dimetyloformamid (C₃H₇NO, DMF), methylene chloride (CH₂Cl₂), and dansyl chloride (DNS-Cl) reagents were purchased from Sigma-Aldrich (Poznan, Poland). All the samples were prepared using deionized water produced by the Hydrolab system installed in our laboratory.

BAs, 2-phenylethylamine (PEA), methylamine (MET), putrescine dihydrochloride (PUT), cadaverine dihydrochloride (CAD), tryptamine (TRY), histamine dihydrochloride (HIS), serotonin hydrochloride (5-HT), tyramine hydrochloride (TYR), dopamine hydrochloride (DA), spermidine trihydrochloride (SPD), and spermine tetrahydrochloride (SPM) were obtained from Sigma-Aldrich (St. Louis, MO, USA). IS, 1,7-diaminoheptane (DMP) was supplied by Sigma-Aldrich (Steinheim, Germany). Dansyl chloride (Dns-Cl) from Sigma-Aldrich (USA) was used as a derivatization reagent. The HPLC grade acetonitrile (ACN) was purchased from Tedia (Fairfield, CT, USA). Deionized water was produced by a MILLPAK Reagent Water System (Millipore, MA, USA). All other reagents were of analytical grade.