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Amicon ultra 15 3k centrifugal filter devices

Manufactured by Merck Group
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The Amicon Ultra 15-3K Centrifugal Filter Devices are lab equipment used for the filtration and concentration of samples. The device features a 3 kDa molecular weight cut-off membrane that allows the separation of small molecules from larger ones during the centrifugation process.

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Lab products found in correlation

Kta fplc system, by GE Healthcare (2 mentions) Histrap hp column, by GE Healthcare (2 mentions) 2 d clean up kit, by Cytiva (1 mentions)

Close spelling variants of this product

Amicon Ultra-15 Centrifugal Filter Devices 3K Ultra Centrifugal Filter Device Amicon Ultra centrifugal filter devices Amicon Ultra-15 Filter Devices Amicon Ultra 3K device Amicon centrifugal filter devices Amicon® Ultra-15 3K Amicon®Ultra-15 device Amicon centrifugal devices 3K) Centrifugal Filters

3 protocols using amicon ultra 15 3k centrifugal filter devices

1

Setdb2 Monoclonal Antibody Generation

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A C-terminal 60 amino acid long sequence (amino acid position 541-600) was fused into a hepatitis B carrier protein as immunogen. This region was amplified by PCR and inserted into a 6x histidine-tagged pB-His HBcAg_Linker plasmid 49 (link). The fusion protein was expressed in E. coli BL21 and purified on 1ml HisTrap HP columns (GE Healthcare) followed by a linear imidazole gradient on an ÄKTA FPLC system (GE Healthcare). The fractions were analyzed by SDS-Page and concentrated with Amicon Ultra 15-3K Centrifugal Filter Devices (Millipore). The immunization and generation of monoclonal B-cell hybridomas was performed by challenging Setdb2GT/GT mice 3 times (every 2 weeks) with 50μg of purified fusion protein antigen mixed 1:1 with adjuvant subcutaneously, before a final immunization intravenously with 50μg purified antigen (adjuvant-free). Mouse sera and clone pools were tested by western blot against overexpressed and endogenous mouse Setdb2. Clone 7H7F11 yielded the best signal-to-noise performance.
Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.
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2

Hs738 Cells Conditioned Medium Effects

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Hs738 cells were cultured at 5 x 104 cells/mL in DMEM supplemented with the indicated concentrations of D-FBS and ITH for the indicated days. The cultured supernatants were collected by centrifugation. To concentrate the medium, Amicon Ultra-15-3K centrifugal filter devices (Millipore) or 2-D clean-up kit (Amersham Biosciences) were used. Gastric cancer cells (3 x 105) were inoculated into 1 mL of the CM of Hs738 cells or assay medium alone in 35 mm dishes and cultured for 1 day. The cells were washed with PBS and the cell lysates were prepared for Western blotting.
Kawada M., Inoue H., Ohba S.I., Yoshida J., Masuda T., Yamasaki M., Usami I., Sakamoto S., Abe H., Watanabe T., Yamori T., Shibasaki M, & Nomoto A. (2015). Stromal Cells Positively and Negatively Modulate the Growth of Cancer Cells: Stimulation via the PGE2-TNFα-IL-6 Pathway and Inhibition via Secreted GAPDH-E-Cadherin Interaction. PLoS ONE, 10(3), e0119415.
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3

Setdb2 Monoclonal Antibody Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols
A C-terminal 60 amino acid long sequence (amino acid position 541-600) was fused into a hepatitis B carrier protein as immunogen. This region was amplified by PCR and inserted into a 6x histidine-tagged pB-His HBcAg_Linker plasmid 49 (link). The fusion protein was expressed in E. coli BL21 and purified on 1ml HisTrap HP columns (GE Healthcare) followed by a linear imidazole gradient on an ÄKTA FPLC system (GE Healthcare). The fractions were analyzed by SDS-Page and concentrated with Amicon Ultra 15-3K Centrifugal Filter Devices (Millipore). The immunization and generation of monoclonal B-cell hybridomas was performed by challenging Setdb2GT/GT mice 3 times (every 2 weeks) with 50μg of purified fusion protein antigen mixed 1:1 with adjuvant subcutaneously, before a final immunization intravenously with 50μg purified antigen (adjuvant-free). Mouse sera and clone pools were tested by western blot against overexpressed and endogenous mouse Setdb2. Clone 7H7F11 yielded the best signal-to-noise performance.
Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.
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